HPLC DETERMINATION OF ETOPOSIDE IN INJECTABLE DOSAGE FORMS
1. The present study describes a direct and an alternative HPLC method for the determination of etoposide in injectable dosage forms. Etoposide, an antineoplastic drug, was eluted through C18 ODS Hypersil column of 250 ÷ 4.6 mm id with 5 ìçé particle size using simple mobile phase of methanol: water...
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Sociedad Chilena de Química
2011
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oai:scielo:S0717-970720110004000122012-03-06HPLC DETERMINATION OF ETOPOSIDE IN INJECTABLE DOSAGE FORMSMUNAWAR HAYAT,MASHRAF,MUHAMMADUR-REHMAN,NISARUL-HASSAN NASIM,FAIZAHMAD,IRSHADRAHMAN,JAMEELSALEEM,MUHAMMADZUBAIR MALIK,M Etoposide HPLC injectable dosage forms nefopam 1. The present study describes a direct and an alternative HPLC method for the determination of etoposide in injectable dosage forms. Etoposide, an antineoplastic drug, was eluted through C18 ODS Hypersil column of 250 ÷ 4.6 mm id with 5 ìçé particle size using simple mobile phase of methanol: water (1:1, v/v) at a flow rate of 1.0 mL min¹ and detected at 204 nm. The limit of detection of etoposide was 0.03 µg mL-1. The proposed method was found linear in the range of 0.25 µg mL¹ to 62.5 µg mL¹ and CV was 1.3% at 0.25 µg mL¹ , 0.48% at 3.90 µg mL¹ and 0.13% at 62.5 µg mL¹. The accuracy of the developed method for two injectable dosage forms was 99.4 and 99.7% with RSD of 0.3%. The validation results and statistical data demonstrate that the method is simple, cost effective, reproducible and suitable in quality analysis of the active ingredient and dosage form.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.56 n.4 20112011-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000400012en10.4067/S0717-97072011000400012 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Etoposide HPLC injectable dosage forms nefopam |
| spellingShingle |
Etoposide HPLC injectable dosage forms nefopam MUNAWAR HAYAT,M ASHRAF,MUHAMMAD UR-REHMAN,NISAR UL-HASSAN NASIM,FAIZ AHMAD,IRSHAD RAHMAN,JAMEEL SALEEM,MUHAMMAD ZUBAIR MALIK,M HPLC DETERMINATION OF ETOPOSIDE IN INJECTABLE DOSAGE FORMS |
| description |
1. The present study describes a direct and an alternative HPLC method for the determination of etoposide in injectable dosage forms. Etoposide, an antineoplastic drug, was eluted through C18 ODS Hypersil column of 250 ÷ 4.6 mm id with 5 ìçé particle size using simple mobile phase of methanol: water (1:1, v/v) at a flow rate of 1.0 mL min¹ and detected at 204 nm. The limit of detection of etoposide was 0.03 µg mL-1. The proposed method was found linear in the range of 0.25 µg mL¹ to 62.5 µg mL¹ and CV was 1.3% at 0.25 µg mL¹ , 0.48% at 3.90 µg mL¹ and 0.13% at 62.5 µg mL¹. The accuracy of the developed method for two injectable dosage forms was 99.4 and 99.7% with RSD of 0.3%. The validation results and statistical data demonstrate that the method is simple, cost effective, reproducible and suitable in quality analysis of the active ingredient and dosage form. |
| author |
MUNAWAR HAYAT,M ASHRAF,MUHAMMAD UR-REHMAN,NISAR UL-HASSAN NASIM,FAIZ AHMAD,IRSHAD RAHMAN,JAMEEL SALEEM,MUHAMMAD ZUBAIR MALIK,M |
| author_facet |
MUNAWAR HAYAT,M ASHRAF,MUHAMMAD UR-REHMAN,NISAR UL-HASSAN NASIM,FAIZ AHMAD,IRSHAD RAHMAN,JAMEEL SALEEM,MUHAMMAD ZUBAIR MALIK,M |
| author_sort |
MUNAWAR HAYAT,M |
| title |
HPLC DETERMINATION OF ETOPOSIDE IN INJECTABLE DOSAGE FORMS |
| title_short |
HPLC DETERMINATION OF ETOPOSIDE IN INJECTABLE DOSAGE FORMS |
| title_full |
HPLC DETERMINATION OF ETOPOSIDE IN INJECTABLE DOSAGE FORMS |
| title_fullStr |
HPLC DETERMINATION OF ETOPOSIDE IN INJECTABLE DOSAGE FORMS |
| title_full_unstemmed |
HPLC DETERMINATION OF ETOPOSIDE IN INJECTABLE DOSAGE FORMS |
| title_sort |
hplc determination of etoposide in injectable dosage forms |
| publisher |
Sociedad Chilena de Química |
| publishDate |
2011 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000400012 |
| work_keys_str_mv |
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| _version_ |
1718445455109521408 |