HPLC DETERMINATION OF ETOPOSIDE IN INJECTABLE DOSAGE FORMS

1. The present study describes a direct and an alternative HPLC method for the determination of etoposide in injectable dosage forms. Etoposide, an antineoplastic drug, was eluted through C18 ODS Hypersil column of 250 ÷ 4.6 mm id with 5 ìçé particle size using simple mobile phase of methanol: water...

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Autores principales: MUNAWAR HAYAT,M, ASHRAF,MUHAMMAD, UR-REHMAN,NISAR, UL-HASSAN NASIM,FAIZ, AHMAD,IRSHAD, RAHMAN,JAMEEL, SALEEM,MUHAMMAD, ZUBAIR MALIK,M
Lenguaje:English
Publicado: Sociedad Chilena de Química 2011
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000400012
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spelling oai:scielo:S0717-970720110004000122012-03-06HPLC DETERMINATION OF ETOPOSIDE IN INJECTABLE DOSAGE FORMSMUNAWAR HAYAT,MASHRAF,MUHAMMADUR-REHMAN,NISARUL-HASSAN NASIM,FAIZAHMAD,IRSHADRAHMAN,JAMEELSALEEM,MUHAMMADZUBAIR MALIK,M Etoposide HPLC injectable dosage forms nefopam 1. The present study describes a direct and an alternative HPLC method for the determination of etoposide in injectable dosage forms. Etoposide, an antineoplastic drug, was eluted through C18 ODS Hypersil column of 250 ÷ 4.6 mm id with 5 ìçé particle size using simple mobile phase of methanol: water (1:1, v/v) at a flow rate of 1.0 mL min¹ and detected at 204 nm. The limit of detection of etoposide was 0.03 µg mL-1. The proposed method was found linear in the range of 0.25 µg mL¹ to 62.5 µg mL¹ and CV was 1.3% at 0.25 µg mL¹ , 0.48% at 3.90 µg mL¹ and 0.13% at 62.5 µg mL¹. The accuracy of the developed method for two injectable dosage forms was 99.4 and 99.7% with RSD of 0.3%. The validation results and statistical data demonstrate that the method is simple, cost effective, reproducible and suitable in quality analysis of the active ingredient and dosage form.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.56 n.4 20112011-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000400012en10.4067/S0717-97072011000400012
institution Scielo Chile
collection Scielo Chile
language English
topic Etoposide
HPLC
injectable dosage forms
nefopam
spellingShingle Etoposide
HPLC
injectable dosage forms
nefopam
MUNAWAR HAYAT,M
ASHRAF,MUHAMMAD
UR-REHMAN,NISAR
UL-HASSAN NASIM,FAIZ
AHMAD,IRSHAD
RAHMAN,JAMEEL
SALEEM,MUHAMMAD
ZUBAIR MALIK,M
HPLC DETERMINATION OF ETOPOSIDE IN INJECTABLE DOSAGE FORMS
description 1. The present study describes a direct and an alternative HPLC method for the determination of etoposide in injectable dosage forms. Etoposide, an antineoplastic drug, was eluted through C18 ODS Hypersil column of 250 ÷ 4.6 mm id with 5 ìçé particle size using simple mobile phase of methanol: water (1:1, v/v) at a flow rate of 1.0 mL min¹ and detected at 204 nm. The limit of detection of etoposide was 0.03 µg mL-1. The proposed method was found linear in the range of 0.25 µg mL¹ to 62.5 µg mL¹ and CV was 1.3% at 0.25 µg mL¹ , 0.48% at 3.90 µg mL¹ and 0.13% at 62.5 µg mL¹. The accuracy of the developed method for two injectable dosage forms was 99.4 and 99.7% with RSD of 0.3%. The validation results and statistical data demonstrate that the method is simple, cost effective, reproducible and suitable in quality analysis of the active ingredient and dosage form.
author MUNAWAR HAYAT,M
ASHRAF,MUHAMMAD
UR-REHMAN,NISAR
UL-HASSAN NASIM,FAIZ
AHMAD,IRSHAD
RAHMAN,JAMEEL
SALEEM,MUHAMMAD
ZUBAIR MALIK,M
author_facet MUNAWAR HAYAT,M
ASHRAF,MUHAMMAD
UR-REHMAN,NISAR
UL-HASSAN NASIM,FAIZ
AHMAD,IRSHAD
RAHMAN,JAMEEL
SALEEM,MUHAMMAD
ZUBAIR MALIK,M
author_sort MUNAWAR HAYAT,M
title HPLC DETERMINATION OF ETOPOSIDE IN INJECTABLE DOSAGE FORMS
title_short HPLC DETERMINATION OF ETOPOSIDE IN INJECTABLE DOSAGE FORMS
title_full HPLC DETERMINATION OF ETOPOSIDE IN INJECTABLE DOSAGE FORMS
title_fullStr HPLC DETERMINATION OF ETOPOSIDE IN INJECTABLE DOSAGE FORMS
title_full_unstemmed HPLC DETERMINATION OF ETOPOSIDE IN INJECTABLE DOSAGE FORMS
title_sort hplc determination of etoposide in injectable dosage forms
publisher Sociedad Chilena de Química
publishDate 2011
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000400012
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