AN AUTOMATED METHOD FOR DETERMINATION OF ASCORBIC ACID IN URINE BY FLOW INJECTION CHEMILUMINESCENCE COUPLING WITH ON-LINE REMOVAL OF INTERFERENCE
A simple and automated method for determination of ascorbic acid (AA) in complex samples by flow injection chemiluminescence (FI-CL) was presented. It is based on the inhibitory effect of ascorbic acid on the chemiluminescence reaction between luminol and hydrogen peroxide in the flow system. A home...
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| Autores principales: | , |
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| Lenguaje: | English |
| Publicado: |
Sociedad Chilena de Química
2011
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| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000400025 |
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| Sumario: | A simple and automated method for determination of ascorbic acid (AA) in complex samples by flow injection chemiluminescence (FI-CL) was presented. It is based on the inhibitory effect of ascorbic acid on the chemiluminescence reaction between luminol and hydrogen peroxide in the flow system. A home-made micro column of cation exchange resin was adapted to the FI manifold to achieve on-line removal of interference. This method was used to determine the AA content in urine samples with a detection limit of 6.03 x10(9) mol/L AA and recovery of 93.1-111.0%. This proposed method allows high - throughput determination of AA in urine samples without chromatographic separation, demonstrating that the FI-CL method has significant potential in determination of species in complex samples by adapting a micro column filled with various functionalized materials to the FI manifold. |
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