AN AUTOMATED METHOD FOR DETERMINATION OF ASCORBIC ACID IN URINE BY FLOW INJECTION CHEMILUMINESCENCE COUPLING WITH ON-LINE REMOVAL OF INTERFERENCE

AN AUTOMATED METHOD FOR DETERMINATION OF ASCORBIC ACID IN URINE BY FLOW INJECTION CHEMILUMINESCENCE COUPLING WITH ON-LINE REMOVAL OF INTERFERENCE

A simple and automated method for determination of ascorbic acid (AA) in complex samples by flow injection chemiluminescence (FI-CL) was presented. It is based on the inhibitory effect of ascorbic acid on the chemiluminescence reaction between luminol and hydrogen peroxide in the flow system. A home...

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Autores principales: CAI,LONGFEI, XU,CHUNXIU
Lenguaje:English
Publicado: Sociedad Chilena de Química 2011
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000400025
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spelling oai:scielo:S0717-970720110004000252012-03-06AN AUTOMATED METHOD FOR DETERMINATION OF ASCORBIC ACID IN URINE BY FLOW INJECTION CHEMILUMINESCENCE COUPLING WITH ON-LINE REMOVAL OF INTERFERENCECAI,LONGFEIXU,CHUNXIUA simple and automated method for determination of ascorbic acid (AA) in complex samples by flow injection chemiluminescence (FI-CL) was presented. It is based on the inhibitory effect of ascorbic acid on the chemiluminescence reaction between luminol and hydrogen peroxide in the flow system. A home-made micro column of cation exchange resin was adapted to the FI manifold to achieve on-line removal of interference. This method was used to determine the AA content in urine samples with a detection limit of 6.03 x10(9) mol/L AA and recovery of 93.1-111.0%. This proposed method allows high - throughput determination of AA in urine samples without chromatographic separation, demonstrating that the FI-CL method has significant potential in determination of species in complex samples by adapting a micro column filled with various functionalized materials to the FI manifold.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.56 n.4 20112011-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000400025en10.4067/S0717-97072011000400025
institution Scielo Chile
collection Scielo Chile
language English
description A simple and automated method for determination of ascorbic acid (AA) in complex samples by flow injection chemiluminescence (FI-CL) was presented. It is based on the inhibitory effect of ascorbic acid on the chemiluminescence reaction between luminol and hydrogen peroxide in the flow system. A home-made micro column of cation exchange resin was adapted to the FI manifold to achieve on-line removal of interference. This method was used to determine the AA content in urine samples with a detection limit of 6.03 x10(9) mol/L AA and recovery of 93.1-111.0%. This proposed method allows high - throughput determination of AA in urine samples without chromatographic separation, demonstrating that the FI-CL method has significant potential in determination of species in complex samples by adapting a micro column filled with various functionalized materials to the FI manifold.
author CAI,LONGFEI
XU,CHUNXIU
spellingShingle CAI,LONGFEI
XU,CHUNXIU
AN AUTOMATED METHOD FOR DETERMINATION OF ASCORBIC ACID IN URINE BY FLOW INJECTION CHEMILUMINESCENCE COUPLING WITH ON-LINE REMOVAL OF INTERFERENCE
author_facet CAI,LONGFEI
XU,CHUNXIU
author_sort CAI,LONGFEI
title AN AUTOMATED METHOD FOR DETERMINATION OF ASCORBIC ACID IN URINE BY FLOW INJECTION CHEMILUMINESCENCE COUPLING WITH ON-LINE REMOVAL OF INTERFERENCE
title_short AN AUTOMATED METHOD FOR DETERMINATION OF ASCORBIC ACID IN URINE BY FLOW INJECTION CHEMILUMINESCENCE COUPLING WITH ON-LINE REMOVAL OF INTERFERENCE
title_full AN AUTOMATED METHOD FOR DETERMINATION OF ASCORBIC ACID IN URINE BY FLOW INJECTION CHEMILUMINESCENCE COUPLING WITH ON-LINE REMOVAL OF INTERFERENCE
title_fullStr AN AUTOMATED METHOD FOR DETERMINATION OF ASCORBIC ACID IN URINE BY FLOW INJECTION CHEMILUMINESCENCE COUPLING WITH ON-LINE REMOVAL OF INTERFERENCE
title_full_unstemmed AN AUTOMATED METHOD FOR DETERMINATION OF ASCORBIC ACID IN URINE BY FLOW INJECTION CHEMILUMINESCENCE COUPLING WITH ON-LINE REMOVAL OF INTERFERENCE
title_sort automated method for determination of ascorbic acid in urine by flow injection chemiluminescence coupling with on-line removal of interference
publisher Sociedad Chilena de Química
publishDate 2011
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000400025
work_keys_str_mv AT cailongfei anautomatedmethodfordeterminationofascorbicacidinurinebyflowinjectionchemiluminescencecouplingwithonlineremovalofinterference
AT xuchunxiu anautomatedmethodfordeterminationofascorbicacidinurinebyflowinjectionchemiluminescencecouplingwithonlineremovalofinterference
AT cailongfei automatedmethodfordeterminationofascorbicacidinurinebyflowinjectionchemiluminescencecouplingwithonlineremovalofinterference
AT xuchunxiu automatedmethodfordeterminationofascorbicacidinurinebyflowinjectionchemiluminescencecouplingwithonlineremovalofinterference
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