TWO SELECTIVE SPECTROPHOTOMETRY METHODS FOR THE DETERMINATION OF THIORIDAZINE HYDROCHLORIDE IN TABLETS AND IN BIOLOGICAL FLUIDS

Two simple, rapid and selective spectrophotometric methods have been developed for the analysis of the psychoactive drug thioridazine HCl (TRH) in tablets and in biological fluids. The first method is based on the oxidation of TRH by a known excess of N- bromosuccinimide (NBS), followed by the deter...

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Autores principales:	EL-DIDAMONY,A. M, MOUSTAFA,M. A
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2012
Materias:	Visible spectrophotometry Thioridazine HCl NBS Sulphonphthalein Dyes Pharmaceutical formulations Biological fluids
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000100006
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spelling	oai:scielo:S0717-970720120001000062012-07-19TWO SELECTIVE SPECTROPHOTOMETRY METHODS FOR THE DETERMINATION OF THIORIDAZINE HYDROCHLORIDE IN TABLETS AND IN BIOLOGICAL FLUIDSEL-DIDAMONY,A. MMOUSTAFA,M. A Visible spectrophotometry Thioridazine HCl NBS Sulphonphthalein Dyes Pharmaceutical formulations Biological fluids Two simple, rapid and selective spectrophotometric methods have been developed for the analysis of the psychoactive drug thioridazine HCl (TRH) in tablets and in biological fluids. The first method is based on the oxidation of TRH by a known excess of N- bromosuccinimide (NBS), followed by the determination of unreacted oxidants by measuring the decrease in absorbance of two different dyes; amaranth (AM) and methylene blue (MB) at a suitable λmax 520 and 660 nm, respectively. Beer-Lambert plots showed good correlation in the concentration ranges of 0.8-4.8 and 0.8-5.6 µg/ml for AM and MB methods, respectively. The second method is based upon the formation of an ion-pair complexes (1: 1) with the acidic sulphonphthalein dyes bromophenol blue (BPB) and bromothymol blue (BTB). The formed complexes were extracted into methylene chloride and their absorbance was measured at 405 and 408 nm for BPB and BTB, respectively. Beer's law was obeyed with a good correlation coefficient (r = 0.9995-0.9989) in the concentration ranges of 4 - 24 µg/ml for both BPB and BTB methods, respectively. All measurements of the two procedures are carried out in an acidic medium at room temperature. Optimizations of the different experimental conditions are described for both methods. The two methods have been successfully applied to the determination of thioridazine HCl in pharmaceutical, serum and urine samples and average recoveries are in the range of 98.12 - 102.55%. Analytical results obtained with this novel method are satisfactory.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.57 n.1 20122012-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000100006en10.4067/S0717-97072012000100006
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Visible spectrophotometry Thioridazine HCl NBS Sulphonphthalein Dyes Pharmaceutical formulations Biological fluids
spellingShingle	Visible spectrophotometry Thioridazine HCl NBS Sulphonphthalein Dyes Pharmaceutical formulations Biological fluids EL-DIDAMONY,A. M MOUSTAFA,M. A TWO SELECTIVE SPECTROPHOTOMETRY METHODS FOR THE DETERMINATION OF THIORIDAZINE HYDROCHLORIDE IN TABLETS AND IN BIOLOGICAL FLUIDS
description	Two simple, rapid and selective spectrophotometric methods have been developed for the analysis of the psychoactive drug thioridazine HCl (TRH) in tablets and in biological fluids. The first method is based on the oxidation of TRH by a known excess of N- bromosuccinimide (NBS), followed by the determination of unreacted oxidants by measuring the decrease in absorbance of two different dyes; amaranth (AM) and methylene blue (MB) at a suitable λmax 520 and 660 nm, respectively. Beer-Lambert plots showed good correlation in the concentration ranges of 0.8-4.8 and 0.8-5.6 µg/ml for AM and MB methods, respectively. The second method is based upon the formation of an ion-pair complexes (1: 1) with the acidic sulphonphthalein dyes bromophenol blue (BPB) and bromothymol blue (BTB). The formed complexes were extracted into methylene chloride and their absorbance was measured at 405 and 408 nm for BPB and BTB, respectively. Beer's law was obeyed with a good correlation coefficient (r = 0.9995-0.9989) in the concentration ranges of 4 - 24 µg/ml for both BPB and BTB methods, respectively. All measurements of the two procedures are carried out in an acidic medium at room temperature. Optimizations of the different experimental conditions are described for both methods. The two methods have been successfully applied to the determination of thioridazine HCl in pharmaceutical, serum and urine samples and average recoveries are in the range of 98.12 - 102.55%. Analytical results obtained with this novel method are satisfactory.
author	EL-DIDAMONY,A. M MOUSTAFA,M. A
author_facet	EL-DIDAMONY,A. M MOUSTAFA,M. A
author_sort	EL-DIDAMONY,A. M
title	TWO SELECTIVE SPECTROPHOTOMETRY METHODS FOR THE DETERMINATION OF THIORIDAZINE HYDROCHLORIDE IN TABLETS AND IN BIOLOGICAL FLUIDS
title_short	TWO SELECTIVE SPECTROPHOTOMETRY METHODS FOR THE DETERMINATION OF THIORIDAZINE HYDROCHLORIDE IN TABLETS AND IN BIOLOGICAL FLUIDS
title_full	TWO SELECTIVE SPECTROPHOTOMETRY METHODS FOR THE DETERMINATION OF THIORIDAZINE HYDROCHLORIDE IN TABLETS AND IN BIOLOGICAL FLUIDS
title_fullStr	TWO SELECTIVE SPECTROPHOTOMETRY METHODS FOR THE DETERMINATION OF THIORIDAZINE HYDROCHLORIDE IN TABLETS AND IN BIOLOGICAL FLUIDS
title_full_unstemmed	TWO SELECTIVE SPECTROPHOTOMETRY METHODS FOR THE DETERMINATION OF THIORIDAZINE HYDROCHLORIDE IN TABLETS AND IN BIOLOGICAL FLUIDS
title_sort	two selective spectrophotometry methods for the determination of thioridazine hydrochloride in tablets and in biological fluids
publisher	Sociedad Chilena de Química
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000100006
work_keys_str_mv	AT eldidamonyam twoselectivespectrophotometrymethodsforthedeterminationofthioridazinehydrochlorideintabletsandinbiologicalfluids AT moustafama twoselectivespectrophotometrymethodsforthedeterminationofthioridazinehydrochlorideintabletsandinbiologicalfluids
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TWO SELECTIVE SPECTROPHOTOMETRY METHODS FOR THE DETERMINATION OF THIORIDAZINE HYDROCHLORIDE IN TABLETS AND IN BIOLOGICAL FLUIDS

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