EXTRACTIVE SPECTROPHOTOMETRIC DETERMINATION OF KETOCONAZOLE, CLOTRIMAZOLE AND FLUCONAZOLE BY ION-PAIR COMPLEX FORMATION WITH BROMOTHYMOL BLUE AND PICRIC ACID
The reactions of picric acid and bromothymol blue with three important antifungal drugs containing an imidazole ring ketoconazole (KC) and clotrimazole (CT) and fluconazole (FC) have been studied for the development of simple, rapid, sensitive an extractive-spectrophotometric method for determining...
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Sociedad Chilena de Química
2012
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oai:scielo:S0717-970720120002000102014-03-10EXTRACTIVE SPECTROPHOTOMETRIC DETERMINATION OF KETOCONAZOLE, CLOTRIMAZOLE AND FLUCONAZOLE BY ION-PAIR COMPLEX FORMATION WITH BROMOTHYMOL BLUE AND PICRIC ACIDALIZADEH,NINAREZAKHANI,ZAHRA Ketoconazole Clotrimazole Fluconazole Bromothymol blue Picric acid Spectrophotometry The reactions of picric acid and bromothymol blue with three important antifungal drugs containing an imidazole ring ketoconazole (KC) and clotrimazole (CT) and fluconazole (FC) have been studied for the development of simple, rapid, sensitive an extractive-spectrophotometric method for determining the concentration of these drugs. This method is based on the formation of yellow ion-pair complexes between the basic nitrogen of the drug and sulphonphthalein acid dyes, namely; bromothymol blue (BTB) in phosphate buffer of pH =3 and picric acid (PA) in citrate buffer of pH=2.5. The formed complexes were extracted with chloroform and measured at 410 and 415nm for KC, at 410 and 413nm for CT and at 373 and 415nm for FC using PA and BTB, respectively. The analytical parameters and their effects on the reported systems are investigated. Beer's law was obeyed in the range 1-60 , 1-58 and 3-60 ìg/ mL with PA and 3-55, 2-50 and 5-55 ìg/ mL with BTB for CT, KC and FC, respectively. The composition of the ion pairs was found 1:1 by mole ratio and job0 s method in all cases. The proposed methods have been applied successfully for the analysis of the studied drugs in pure forms and pharmaceutical formulations.The results are in good agreement with those obtained by official methods.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.57 n.2 20122012-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000200010en10.4067/S0717-97072012000200010 |
institution |
Scielo Chile |
collection |
Scielo Chile |
language |
English |
topic |
Ketoconazole Clotrimazole Fluconazole Bromothymol blue Picric acid Spectrophotometry |
spellingShingle |
Ketoconazole Clotrimazole Fluconazole Bromothymol blue Picric acid Spectrophotometry ALIZADEH,NINA REZAKHANI,ZAHRA EXTRACTIVE SPECTROPHOTOMETRIC DETERMINATION OF KETOCONAZOLE, CLOTRIMAZOLE AND FLUCONAZOLE BY ION-PAIR COMPLEX FORMATION WITH BROMOTHYMOL BLUE AND PICRIC ACID |
description |
The reactions of picric acid and bromothymol blue with three important antifungal drugs containing an imidazole ring ketoconazole (KC) and clotrimazole (CT) and fluconazole (FC) have been studied for the development of simple, rapid, sensitive an extractive-spectrophotometric method for determining the concentration of these drugs. This method is based on the formation of yellow ion-pair complexes between the basic nitrogen of the drug and sulphonphthalein acid dyes, namely; bromothymol blue (BTB) in phosphate buffer of pH =3 and picric acid (PA) in citrate buffer of pH=2.5. The formed complexes were extracted with chloroform and measured at 410 and 415nm for KC, at 410 and 413nm for CT and at 373 and 415nm for FC using PA and BTB, respectively. The analytical parameters and their effects on the reported systems are investigated. Beer's law was obeyed in the range 1-60 , 1-58 and 3-60 ìg/ mL with PA and 3-55, 2-50 and 5-55 ìg/ mL with BTB for CT, KC and FC, respectively. The composition of the ion pairs was found 1:1 by mole ratio and job0 s method in all cases. The proposed methods have been applied successfully for the analysis of the studied drugs in pure forms and pharmaceutical formulations.The results are in good agreement with those obtained by official methods. |
author |
ALIZADEH,NINA REZAKHANI,ZAHRA |
author_facet |
ALIZADEH,NINA REZAKHANI,ZAHRA |
author_sort |
ALIZADEH,NINA |
title |
EXTRACTIVE SPECTROPHOTOMETRIC DETERMINATION OF KETOCONAZOLE, CLOTRIMAZOLE AND FLUCONAZOLE BY ION-PAIR COMPLEX FORMATION WITH BROMOTHYMOL BLUE AND PICRIC ACID |
title_short |
EXTRACTIVE SPECTROPHOTOMETRIC DETERMINATION OF KETOCONAZOLE, CLOTRIMAZOLE AND FLUCONAZOLE BY ION-PAIR COMPLEX FORMATION WITH BROMOTHYMOL BLUE AND PICRIC ACID |
title_full |
EXTRACTIVE SPECTROPHOTOMETRIC DETERMINATION OF KETOCONAZOLE, CLOTRIMAZOLE AND FLUCONAZOLE BY ION-PAIR COMPLEX FORMATION WITH BROMOTHYMOL BLUE AND PICRIC ACID |
title_fullStr |
EXTRACTIVE SPECTROPHOTOMETRIC DETERMINATION OF KETOCONAZOLE, CLOTRIMAZOLE AND FLUCONAZOLE BY ION-PAIR COMPLEX FORMATION WITH BROMOTHYMOL BLUE AND PICRIC ACID |
title_full_unstemmed |
EXTRACTIVE SPECTROPHOTOMETRIC DETERMINATION OF KETOCONAZOLE, CLOTRIMAZOLE AND FLUCONAZOLE BY ION-PAIR COMPLEX FORMATION WITH BROMOTHYMOL BLUE AND PICRIC ACID |
title_sort |
extractive spectrophotometric determination of ketoconazole, clotrimazole and fluconazole by ion-pair complex formation with bromothymol blue and picric acid |
publisher |
Sociedad Chilena de Química |
publishDate |
2012 |
url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000200010 |
work_keys_str_mv |
AT alizadehnina extractivespectrophotometricdeterminationofketoconazoleclotrimazoleandfluconazolebyionpaircomplexformationwithbromothymolblueandpicricacid AT rezakhanizahra extractivespectrophotometricdeterminationofketoconazoleclotrimazoleandfluconazolebyionpaircomplexformationwithbromothymolblueandpicricacid |
_version_ |
1718445464231084032 |