SIMULTANEOUS DETERMINATION OF PIPERACILLIN AND TAZOBACTAM IN THE PHARMACEUTICAL FORMULATION TAZONAM® BY DERIVATIVE SPECTROPHOTOMETRY

In this paper, a simple and rapid method was developed for the simultaneous determination of Piperacillin (PIP) and Tazobactam (TAZ) by second-order derivative spectrophotometry. Water was selected as a solvent for extraction and determination of both analytes. Further studies of photo-stability and...

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Autores principales:	TORAL,M. INÉS, NOVA-RAMÍREZ,FRANCISCA, NACARATTE,FALLON
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2012
Materias:	UV-vis derivative spectrophotometry Piperacillin Tazobactam Pharmaceutical formulation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000200028
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spelling	oai:scielo:S0717-970720120002000282014-03-10SIMULTANEOUS DETERMINATION OF PIPERACILLIN AND TAZOBACTAM IN THE PHARMACEUTICAL FORMULATION TAZONAM® BY DERIVATIVE SPECTROPHOTOMETRYTORAL,M. INÉSNOVA-RAMÍREZ,FRANCISCANACARATTE,FALLON UV-vis derivative spectrophotometry Piperacillin Tazobactam Pharmaceutical formulation In this paper, a simple and rapid method was developed for the simultaneous determination of Piperacillin (PIP) and Tazobactam (TAZ) by second-order derivative spectrophotometry. Water was selected as a solvent for extraction and determination of both analytes. Further studies of photo-stability and forced degradation for both drugs were also performed. The spectral variables were optimized, a smoothing factor of 8·10³ and a scaling factor of 1·10(4) were selected and the analytical signals were evaluated at 296.3 nm and 233.3 nm for PIP and TAZ, respectively. The detection and quantification limits for PIP and TAZ were: 3.210-7 to 9.510-7 and 2.710-7 to 8.910-7 mol/L, respectively. The levels of repeatability (RSD) were 1.5% and 2.4% for PIP and TAZ respectively. The method was applied to the pharmaceutical formulation TAZONAM NF® Wyeth. USA, with a nominal content of 4.0 g of PIP, 0.5 g of TAZ, 0.139 g of citric acid and 1 mg of EDTA. A study of excipients was carried out and it was found that they do not interfere with quantification. By applying this method, a content of 3.96 ± 0.04 g and 0.57 ± 0.01 g was found of PIP and TAZ, respectively.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.57 n.2 20122012-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000200028en10.4067/S0717-97072012000200028
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	UV-vis derivative spectrophotometry Piperacillin Tazobactam Pharmaceutical formulation
spellingShingle	UV-vis derivative spectrophotometry Piperacillin Tazobactam Pharmaceutical formulation TORAL,M. INÉS NOVA-RAMÍREZ,FRANCISCA NACARATTE,FALLON SIMULTANEOUS DETERMINATION OF PIPERACILLIN AND TAZOBACTAM IN THE PHARMACEUTICAL FORMULATION TAZONAM® BY DERIVATIVE SPECTROPHOTOMETRY
description	In this paper, a simple and rapid method was developed for the simultaneous determination of Piperacillin (PIP) and Tazobactam (TAZ) by second-order derivative spectrophotometry. Water was selected as a solvent for extraction and determination of both analytes. Further studies of photo-stability and forced degradation for both drugs were also performed. The spectral variables were optimized, a smoothing factor of 8·10³ and a scaling factor of 1·10(4) were selected and the analytical signals were evaluated at 296.3 nm and 233.3 nm for PIP and TAZ, respectively. The detection and quantification limits for PIP and TAZ were: 3.210-7 to 9.510-7 and 2.710-7 to 8.910-7 mol/L, respectively. The levels of repeatability (RSD) were 1.5% and 2.4% for PIP and TAZ respectively. The method was applied to the pharmaceutical formulation TAZONAM NF® Wyeth. USA, with a nominal content of 4.0 g of PIP, 0.5 g of TAZ, 0.139 g of citric acid and 1 mg of EDTA. A study of excipients was carried out and it was found that they do not interfere with quantification. By applying this method, a content of 3.96 ± 0.04 g and 0.57 ± 0.01 g was found of PIP and TAZ, respectively.
author	TORAL,M. INÉS NOVA-RAMÍREZ,FRANCISCA NACARATTE,FALLON
author_facet	TORAL,M. INÉS NOVA-RAMÍREZ,FRANCISCA NACARATTE,FALLON
author_sort	TORAL,M. INÉS
title	SIMULTANEOUS DETERMINATION OF PIPERACILLIN AND TAZOBACTAM IN THE PHARMACEUTICAL FORMULATION TAZONAM® BY DERIVATIVE SPECTROPHOTOMETRY
title_short	SIMULTANEOUS DETERMINATION OF PIPERACILLIN AND TAZOBACTAM IN THE PHARMACEUTICAL FORMULATION TAZONAM® BY DERIVATIVE SPECTROPHOTOMETRY
title_full	SIMULTANEOUS DETERMINATION OF PIPERACILLIN AND TAZOBACTAM IN THE PHARMACEUTICAL FORMULATION TAZONAM® BY DERIVATIVE SPECTROPHOTOMETRY
title_fullStr	SIMULTANEOUS DETERMINATION OF PIPERACILLIN AND TAZOBACTAM IN THE PHARMACEUTICAL FORMULATION TAZONAM® BY DERIVATIVE SPECTROPHOTOMETRY
title_full_unstemmed	SIMULTANEOUS DETERMINATION OF PIPERACILLIN AND TAZOBACTAM IN THE PHARMACEUTICAL FORMULATION TAZONAM® BY DERIVATIVE SPECTROPHOTOMETRY
title_sort	simultaneous determination of piperacillin and tazobactam in the pharmaceutical formulation tazonam® by derivative spectrophotometry
publisher	Sociedad Chilena de Química
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000200028
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SIMULTANEOUS DETERMINATION OF PIPERACILLIN AND TAZOBACTAM IN THE PHARMACEUTICAL FORMULATION TAZONAM® BY DERIVATIVE SPECTROPHOTOMETRY

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