IMMOBILIZATION OF GLUCOSE OXIDASE IN SILICA SOL-GEL FILM FOR APPLICATION TO BIOSENSOR AND AMPEROMETRIC DETERMINATION OF GLUCOSE

In this study, an electrochemical biosensor was developed by using a sol-gel coating solution. The modified platinum electrode used in the study was constructed by immobilization of glucose oxidase under a layer of sol-gel film. The sol-gel coating solution was prepared by using GLYMO, TEOS, and MTE...

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Autores principales:	DEMİRKIRAN,NİZAMETTİN, EKİNCİ,ERGUN, ASİLTÜRK,MELTEM
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2012
Materias:	Amperometry biosensor glucose glucose oxidase sol-gel
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000400001
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spelling	oai:scielo:S0717-970720120004000012013-04-04IMMOBILIZATION OF GLUCOSE OXIDASE IN SILICA SOL-GEL FILM FOR APPLICATION TO BIOSENSOR AND AMPEROMETRIC DETERMINATION OF GLUCOSEDEMİRKIRAN,NİZAMETTİNEKİNCİ,ERGUNASİLTÜRK,MELTEM Amperometry biosensor glucose glucose oxidase sol-gel In this study, an electrochemical biosensor was developed by using a sol-gel coating solution. The modified platinum electrode used in the study was constructed by immobilization of glucose oxidase under a layer of sol-gel film. The sol-gel coating solution was prepared by using GLYMO, TEOS, and MTEOS. Electrochemical measurements were carried out amperometrically by determining hydrogen peroxide produced by the enzymatic reaction between glucose and glucose oxidase. The amperometric responses of the resulting enzymatic electrode to glucose were rapid. It was observed that the amperometric response of the enzymatic electrode was linear for glucose concentrations in the range from 2 to18 mM with 50 s response time. LOD and LOQ for the enzymatic electrode were calculated to be 0.055 mM and 0.184 mM, respectively. It was determined that the developed biosensor had an acceptable reproducibility. The selectivity of the biosensor was determined in the presence of some interfering substances, such as lactose, sucrose, urea, uric acid, oxalic acid, and ascorbic acid. The stability of the biosensor was investigated, and it was found that the sensor response decreased by 59% of its initial response over a period of 30 days of storage in dry conditions at 4 ºC.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.57 n.4 20122012-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000400001en10.4067/S0717-97072012000400001
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Amperometry biosensor glucose glucose oxidase sol-gel
spellingShingle	Amperometry biosensor glucose glucose oxidase sol-gel DEMİRKIRAN,NİZAMETTİN EKİNCİ,ERGUN ASİLTÜRK,MELTEM IMMOBILIZATION OF GLUCOSE OXIDASE IN SILICA SOL-GEL FILM FOR APPLICATION TO BIOSENSOR AND AMPEROMETRIC DETERMINATION OF GLUCOSE
description	In this study, an electrochemical biosensor was developed by using a sol-gel coating solution. The modified platinum electrode used in the study was constructed by immobilization of glucose oxidase under a layer of sol-gel film. The sol-gel coating solution was prepared by using GLYMO, TEOS, and MTEOS. Electrochemical measurements were carried out amperometrically by determining hydrogen peroxide produced by the enzymatic reaction between glucose and glucose oxidase. The amperometric responses of the resulting enzymatic electrode to glucose were rapid. It was observed that the amperometric response of the enzymatic electrode was linear for glucose concentrations in the range from 2 to18 mM with 50 s response time. LOD and LOQ for the enzymatic electrode were calculated to be 0.055 mM and 0.184 mM, respectively. It was determined that the developed biosensor had an acceptable reproducibility. The selectivity of the biosensor was determined in the presence of some interfering substances, such as lactose, sucrose, urea, uric acid, oxalic acid, and ascorbic acid. The stability of the biosensor was investigated, and it was found that the sensor response decreased by 59% of its initial response over a period of 30 days of storage in dry conditions at 4 ºC.
author	DEMİRKIRAN,NİZAMETTİN EKİNCİ,ERGUN ASİLTÜRK,MELTEM
author_facet	DEMİRKIRAN,NİZAMETTİN EKİNCİ,ERGUN ASİLTÜRK,MELTEM
author_sort	DEMİRKIRAN,NİZAMETTİN
title	IMMOBILIZATION OF GLUCOSE OXIDASE IN SILICA SOL-GEL FILM FOR APPLICATION TO BIOSENSOR AND AMPEROMETRIC DETERMINATION OF GLUCOSE
title_short	IMMOBILIZATION OF GLUCOSE OXIDASE IN SILICA SOL-GEL FILM FOR APPLICATION TO BIOSENSOR AND AMPEROMETRIC DETERMINATION OF GLUCOSE
title_full	IMMOBILIZATION OF GLUCOSE OXIDASE IN SILICA SOL-GEL FILM FOR APPLICATION TO BIOSENSOR AND AMPEROMETRIC DETERMINATION OF GLUCOSE
title_fullStr	IMMOBILIZATION OF GLUCOSE OXIDASE IN SILICA SOL-GEL FILM FOR APPLICATION TO BIOSENSOR AND AMPEROMETRIC DETERMINATION OF GLUCOSE
title_full_unstemmed	IMMOBILIZATION OF GLUCOSE OXIDASE IN SILICA SOL-GEL FILM FOR APPLICATION TO BIOSENSOR AND AMPEROMETRIC DETERMINATION OF GLUCOSE
title_sort	immobilization of glucose oxidase in silica sol-gel film for application to biosensor and amperometric determination of glucose
publisher	Sociedad Chilena de Química
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000400001
work_keys_str_mv	AT dem304rkirann304zamett304n immobilizationofglucoseoxidaseinsilicasolgelfilmforapplicationtobiosensorandamperometricdeterminationofglucose AT ek304nc304ergun immobilizationofglucoseoxidaseinsilicasolgelfilmforapplicationtobiosensorandamperometricdeterminationofglucose AT as304lturkmeltem immobilizationofglucoseoxidaseinsilicasolgelfilmforapplicationtobiosensorandamperometricdeterminationofglucose
_version_	1718445472974110720

IMMOBILIZATION OF GLUCOSE OXIDASE IN SILICA SOL-GEL FILM FOR APPLICATION TO BIOSENSOR AND AMPEROMETRIC DETERMINATION OF GLUCOSE

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