IN VITRO CHARACTERIZATION OF ANTIOXIDANT PROPERTIES OF CUBAN ENDEMIC VARIETIES OF Erythroxylum alaternifolium A. Rich. ISOLATION OF TWO FLAVONOL GLYCOSIDES
The total antioxidant capacity from leaves of three Cuban endemic varieties of Erythroxylum alaternifolium was measured using three techniques: 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) and Oxygen radical absorbance capacity (ORAC). The...
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| Autores principales: | , , , , , |
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| Lenguaje: | English |
| Publicado: |
Sociedad Chilena de Química
2012
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| Materias: | |
| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000400002 |
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| Sumario: | The total antioxidant capacity from leaves of three Cuban endemic varieties of Erythroxylum alaternifolium was measured using three techniques: 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) and Oxygen radical absorbance capacity (ORAC). The highest values of antioxidant capacity and total phenols were measured in polar n-butyl alcohol extracts. Among the varieties of E. alaternifolium assayed, the var. parvifolium showed the strongest antioxidant capacity: ABTS (6.49 ± 0.05 mg TE/g dw); DPPH (11.16 ± 0.01 mg TE/g dw) and ORAC (35.1 ± 1.5 mg TE/g dw). Additionally, n-butyl alcohol extract of E. alaternifolium var. parvifolium also showed the highest content in phenolics with 284.2 ± 7.3 mg CAE/g dw and in flavonols with skeleton quercetin, kaempferol and myricetin with 471 μg/g dw. Moreover, two flavonol glycosides: ombuin-3-O-rutinoside and quercetin-3-O-rutinoside were isolated for the first time from n-butyl alcohol extract of E. alaternifolium var. alaternifolium. |
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