IN VITRO CHARACTERIZATION OF ANTIOXIDANT PROPERTIES OF CUBAN ENDEMIC VARIETIES OF Erythroxylum alaternifolium A. Rich. ISOLATION OF TWO FLAVONOL GLYCOSIDES

The total antioxidant capacity from leaves of three Cuban endemic varieties of Erythroxylum alaternifolium was measured using three techniques: 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) and Oxygen radical absorbance capacity (ORAC). The...

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Autores principales: PERERA CÓRDOVA,WILMER H, GÓMEZ MATOS,MISLÉN, TABART,JESSICA, SIPEL,ARNAUD, KEVERS,CLAIRE, DOMMES,JACQUES
Lenguaje:English
Publicado: Sociedad Chilena de Química 2012
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000400002
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spelling oai:scielo:S0717-970720120004000022013-04-04IN VITRO CHARACTERIZATION OF ANTIOXIDANT PROPERTIES OF CUBAN ENDEMIC VARIETIES OF Erythroxylum alaternifolium A. Rich. ISOLATION OF TWO FLAVONOL GLYCOSIDESPERERA CÓRDOVA,WILMER HGÓMEZ MATOS,MISLÉNTABART,JESSICASIPEL,ARNAUDKEVERS,CLAIREDOMMES,JACQUES Erythroxylum Antioxidant assays Flavonols aglycone Ombuin-3-O-rutinoside Quercetin-3-O-rutinoside The total antioxidant capacity from leaves of three Cuban endemic varieties of Erythroxylum alaternifolium was measured using three techniques: 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) and Oxygen radical absorbance capacity (ORAC). The highest values of antioxidant capacity and total phenols were measured in polar n-butyl alcohol extracts. Among the varieties of E. alaternifolium assayed, the var. parvifolium showed the strongest antioxidant capacity: ABTS (6.49 ± 0.05 mg TE/g dw); DPPH (11.16 ± 0.01 mg TE/g dw) and ORAC (35.1 ± 1.5 mg TE/g dw). Additionally, n-butyl alcohol extract of E. alaternifolium var. parvifolium also showed the highest content in phenolics with 284.2 ± 7.3 mg CAE/g dw and in flavonols with skeleton quercetin, kaempferol and myricetin with 471 μg/g dw. Moreover, two flavonol glycosides: ombuin-3-O-rutinoside and quercetin-3-O-rutinoside were isolated for the first time from n-butyl alcohol extract of E. alaternifolium var. alaternifolium.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.57 n.4 20122012-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000400002en10.4067/S0717-97072012000400002
institution Scielo Chile
collection Scielo Chile
language English
topic Erythroxylum
Antioxidant assays
Flavonols aglycone
Ombuin-3-O-rutinoside
Quercetin-3-O-rutinoside
spellingShingle Erythroxylum
Antioxidant assays
Flavonols aglycone
Ombuin-3-O-rutinoside
Quercetin-3-O-rutinoside
PERERA CÓRDOVA,WILMER H
GÓMEZ MATOS,MISLÉN
TABART,JESSICA
SIPEL,ARNAUD
KEVERS,CLAIRE
DOMMES,JACQUES
IN VITRO CHARACTERIZATION OF ANTIOXIDANT PROPERTIES OF CUBAN ENDEMIC VARIETIES OF Erythroxylum alaternifolium A. Rich. ISOLATION OF TWO FLAVONOL GLYCOSIDES
description The total antioxidant capacity from leaves of three Cuban endemic varieties of Erythroxylum alaternifolium was measured using three techniques: 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) and Oxygen radical absorbance capacity (ORAC). The highest values of antioxidant capacity and total phenols were measured in polar n-butyl alcohol extracts. Among the varieties of E. alaternifolium assayed, the var. parvifolium showed the strongest antioxidant capacity: ABTS (6.49 ± 0.05 mg TE/g dw); DPPH (11.16 ± 0.01 mg TE/g dw) and ORAC (35.1 ± 1.5 mg TE/g dw). Additionally, n-butyl alcohol extract of E. alaternifolium var. parvifolium also showed the highest content in phenolics with 284.2 ± 7.3 mg CAE/g dw and in flavonols with skeleton quercetin, kaempferol and myricetin with 471 μg/g dw. Moreover, two flavonol glycosides: ombuin-3-O-rutinoside and quercetin-3-O-rutinoside were isolated for the first time from n-butyl alcohol extract of E. alaternifolium var. alaternifolium.
author PERERA CÓRDOVA,WILMER H
GÓMEZ MATOS,MISLÉN
TABART,JESSICA
SIPEL,ARNAUD
KEVERS,CLAIRE
DOMMES,JACQUES
author_facet PERERA CÓRDOVA,WILMER H
GÓMEZ MATOS,MISLÉN
TABART,JESSICA
SIPEL,ARNAUD
KEVERS,CLAIRE
DOMMES,JACQUES
author_sort PERERA CÓRDOVA,WILMER H
title IN VITRO CHARACTERIZATION OF ANTIOXIDANT PROPERTIES OF CUBAN ENDEMIC VARIETIES OF Erythroxylum alaternifolium A. Rich. ISOLATION OF TWO FLAVONOL GLYCOSIDES
title_short IN VITRO CHARACTERIZATION OF ANTIOXIDANT PROPERTIES OF CUBAN ENDEMIC VARIETIES OF Erythroxylum alaternifolium A. Rich. ISOLATION OF TWO FLAVONOL GLYCOSIDES
title_full IN VITRO CHARACTERIZATION OF ANTIOXIDANT PROPERTIES OF CUBAN ENDEMIC VARIETIES OF Erythroxylum alaternifolium A. Rich. ISOLATION OF TWO FLAVONOL GLYCOSIDES
title_fullStr IN VITRO CHARACTERIZATION OF ANTIOXIDANT PROPERTIES OF CUBAN ENDEMIC VARIETIES OF Erythroxylum alaternifolium A. Rich. ISOLATION OF TWO FLAVONOL GLYCOSIDES
title_full_unstemmed IN VITRO CHARACTERIZATION OF ANTIOXIDANT PROPERTIES OF CUBAN ENDEMIC VARIETIES OF Erythroxylum alaternifolium A. Rich. ISOLATION OF TWO FLAVONOL GLYCOSIDES
title_sort in vitro characterization of antioxidant properties of cuban endemic varieties of erythroxylum alaternifolium a. rich. isolation of two flavonol glycosides
publisher Sociedad Chilena de Química
publishDate 2012
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000400002
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