SIMULTANEOUS ESTIMATION OF MOXIFLOXACIN HYDROCHLORIDE AND DEXAMETHASONE SODIUM PHOSPHATE IN BULK AND IN OPHTHALMIC SOLUTION BY RP- HPLC

A new simple, precise, accurate and selective RP-HPLC method has been developed and validated for simultaneous estimation of Moxifloxacin Hydrochloride (MOX) and Dexamethasone Sodium Phosphate (DSP) in Ophthalmic Solution. The method was carried out on a Qualisil RP C-8 (250 mm x 4.6 mm, 5 µm) colum...

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Autores principales:	DHUMAL,D. M, SHIRKHEDKAR,A. A, NERKAR,P. P, SURANA,S. J
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2012
Materias:	Moxifloxacin Hydrochloride Dexamethasone Sodium Phosphate RP-HPLC
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000400003
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spelling	oai:scielo:S0717-970720120004000032013-04-04SIMULTANEOUS ESTIMATION OF MOXIFLOXACIN HYDROCHLORIDE AND DEXAMETHASONE SODIUM PHOSPHATE IN BULK AND IN OPHTHALMIC SOLUTION BY RP- HPLCDHUMAL,D. MSHIRKHEDKAR,A. ANERKAR,P. PSURANA,S. J Moxifloxacin Hydrochloride Dexamethasone Sodium Phosphate RP-HPLC A new simple, precise, accurate and selective RP-HPLC method has been developed and validated for simultaneous estimation of Moxifloxacin Hydrochloride (MOX) and Dexamethasone Sodium Phosphate (DSP) in Ophthalmic Solution. The method was carried out on a Qualisil RP C-8 (250 mm x 4.6 mm, 5 µm) column with a mobile phase consisting of Methanol: Water (75:25 v/v) pH adjusted to 3.0 with ortho-phosphoric acid of aqueous phase and flow rate of 1.0 mL min¹. Detection was carried out at 240 nm. The retention time for MOX and DSP was found to be 2.22, and 7.26 min, respectively. The MOX and DSP followed linearity in the concentration range of 10 - 60 µg mL-1 and 2- 12 µg mL-1 with r²= 0.99, respectively. The amounts of both these drugs estimated by proposed method were found to be in good agreement with label claim. The developed method was validated for sensitivity, accuracy, precision, ruggedness and robustness. The LOD and LOQ were found to be 0.30 µg mL-1 and 0.91 µg mL-1 for MOX and 0.10 µg mL-1 and 0.30 µg mL-1 for DSP. The proposed method can be used for routine analysis of both these drugs simultaneously in their combined dosage form.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.57 n.4 20122012-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000400003en10.4067/S0717-97072012000400003
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Moxifloxacin Hydrochloride Dexamethasone Sodium Phosphate RP-HPLC
spellingShingle	Moxifloxacin Hydrochloride Dexamethasone Sodium Phosphate RP-HPLC DHUMAL,D. M SHIRKHEDKAR,A. A NERKAR,P. P SURANA,S. J SIMULTANEOUS ESTIMATION OF MOXIFLOXACIN HYDROCHLORIDE AND DEXAMETHASONE SODIUM PHOSPHATE IN BULK AND IN OPHTHALMIC SOLUTION BY RP- HPLC
description	A new simple, precise, accurate and selective RP-HPLC method has been developed and validated for simultaneous estimation of Moxifloxacin Hydrochloride (MOX) and Dexamethasone Sodium Phosphate (DSP) in Ophthalmic Solution. The method was carried out on a Qualisil RP C-8 (250 mm x 4.6 mm, 5 µm) column with a mobile phase consisting of Methanol: Water (75:25 v/v) pH adjusted to 3.0 with ortho-phosphoric acid of aqueous phase and flow rate of 1.0 mL min¹. Detection was carried out at 240 nm. The retention time for MOX and DSP was found to be 2.22, and 7.26 min, respectively. The MOX and DSP followed linearity in the concentration range of 10 - 60 µg mL-1 and 2- 12 µg mL-1 with r²= 0.99, respectively. The amounts of both these drugs estimated by proposed method were found to be in good agreement with label claim. The developed method was validated for sensitivity, accuracy, precision, ruggedness and robustness. The LOD and LOQ were found to be 0.30 µg mL-1 and 0.91 µg mL-1 for MOX and 0.10 µg mL-1 and 0.30 µg mL-1 for DSP. The proposed method can be used for routine analysis of both these drugs simultaneously in their combined dosage form.
author	DHUMAL,D. M SHIRKHEDKAR,A. A NERKAR,P. P SURANA,S. J
author_facet	DHUMAL,D. M SHIRKHEDKAR,A. A NERKAR,P. P SURANA,S. J
author_sort	DHUMAL,D. M
title	SIMULTANEOUS ESTIMATION OF MOXIFLOXACIN HYDROCHLORIDE AND DEXAMETHASONE SODIUM PHOSPHATE IN BULK AND IN OPHTHALMIC SOLUTION BY RP- HPLC
title_short	SIMULTANEOUS ESTIMATION OF MOXIFLOXACIN HYDROCHLORIDE AND DEXAMETHASONE SODIUM PHOSPHATE IN BULK AND IN OPHTHALMIC SOLUTION BY RP- HPLC
title_full	SIMULTANEOUS ESTIMATION OF MOXIFLOXACIN HYDROCHLORIDE AND DEXAMETHASONE SODIUM PHOSPHATE IN BULK AND IN OPHTHALMIC SOLUTION BY RP- HPLC
title_fullStr	SIMULTANEOUS ESTIMATION OF MOXIFLOXACIN HYDROCHLORIDE AND DEXAMETHASONE SODIUM PHOSPHATE IN BULK AND IN OPHTHALMIC SOLUTION BY RP- HPLC
title_full_unstemmed	SIMULTANEOUS ESTIMATION OF MOXIFLOXACIN HYDROCHLORIDE AND DEXAMETHASONE SODIUM PHOSPHATE IN BULK AND IN OPHTHALMIC SOLUTION BY RP- HPLC
title_sort	simultaneous estimation of moxifloxacin hydrochloride and dexamethasone sodium phosphate in bulk and in ophthalmic solution by rp- hplc
publisher	Sociedad Chilena de Química
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000400003
work_keys_str_mv	AT dhumaldm simultaneousestimationofmoxifloxacinhydrochlorideanddexamethasonesodiumphosphateinbulkandinophthalmicsolutionbyrphplc AT shirkhedkaraa simultaneousestimationofmoxifloxacinhydrochlorideanddexamethasonesodiumphosphateinbulkandinophthalmicsolutionbyrphplc AT nerkarpp simultaneousestimationofmoxifloxacinhydrochlorideanddexamethasonesodiumphosphateinbulkandinophthalmicsolutionbyrphplc AT suranasj simultaneousestimationofmoxifloxacinhydrochlorideanddexamethasonesodiumphosphateinbulkandinophthalmicsolutionbyrphplc
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SIMULTANEOUS ESTIMATION OF MOXIFLOXACIN HYDROCHLORIDE AND DEXAMETHASONE SODIUM PHOSPHATE IN BULK AND IN OPHTHALMIC SOLUTION BY RP- HPLC

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