DETERMINATION OF CHLOROGENIC ACID, BAICALIN AND FORSYTHIN IN SHUANGHUANGLIAN PREPARATIONS BY HPLC-DAD

High performance liquid chromatography coupled with diode array detection (HPLC-DAD) was developed for the simultaneous determination of three bioactive components - chlorogenic acid, baicalin and forsythin - in traditional Chinese medicinal Shuanghuanglian preparations. The analytes were extracted...

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Autores principales:	YANG,LI, YAN,QINGHUA, MO,HAIZHEN, LI,XIAOMIN, WANG,QING
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2012
Materias:	HPLC-DAD Shuanghuanglian preparation Chlorogenic acid Baicalin Forsythin
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000400006
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spelling	oai:scielo:S0717-970720120004000062013-04-04DETERMINATION OF CHLOROGENIC ACID, BAICALIN AND FORSYTHIN IN SHUANGHUANGLIAN PREPARATIONS BY HPLC-DADYANG,LIYAN,QINGHUAMO,HAIZHENLI,XIAOMINWANG,QING HPLC-DAD Shuanghuanglian preparation Chlorogenic acid Baicalin Forsythin High performance liquid chromatography coupled with diode array detection (HPLC-DAD) was developed for the simultaneous determination of three bioactive components - chlorogenic acid, baicalin and forsythin - in traditional Chinese medicinal Shuanghuanglian preparations. The analytes were extracted successfully using 80% aqueous methanol for 15 min in an ultrasonic bath. The optical chromatographic separation conditions were performed on a Finnigan Surveyor HPLC system with an Agilent Zorbax ODS C18 (4.6x150 mm, particle size 5 ìçé ) at 30 ºC using a gradient elution program with a mixture of acetonitrile and 0.2% phosphoric acid (v/v) as mobile phase within 25 min. Method validation results were obtained through the generation of calibration curves with the mixed standard solutions and a standard addition method, regression equations revealed linear relationships (correlation coefficients 0.9965-0.9985) between the peak area and concentration of the three analytes. The average recoveries were 90-106% (n= 6) with standard deviation below 3.37%. The developed HPLC assay was specific and could be readily utilized for comprehensive evaluation of Shuanghuanglian preparations.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.57 n.4 20122012-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000400006en10.4067/S0717-97072012000400006
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	HPLC-DAD Shuanghuanglian preparation Chlorogenic acid Baicalin Forsythin
spellingShingle	HPLC-DAD Shuanghuanglian preparation Chlorogenic acid Baicalin Forsythin YANG,LI YAN,QINGHUA MO,HAIZHEN LI,XIAOMIN WANG,QING DETERMINATION OF CHLOROGENIC ACID, BAICALIN AND FORSYTHIN IN SHUANGHUANGLIAN PREPARATIONS BY HPLC-DAD
description	High performance liquid chromatography coupled with diode array detection (HPLC-DAD) was developed for the simultaneous determination of three bioactive components - chlorogenic acid, baicalin and forsythin - in traditional Chinese medicinal Shuanghuanglian preparations. The analytes were extracted successfully using 80% aqueous methanol for 15 min in an ultrasonic bath. The optical chromatographic separation conditions were performed on a Finnigan Surveyor HPLC system with an Agilent Zorbax ODS C18 (4.6x150 mm, particle size 5 ìçé ) at 30 ºC using a gradient elution program with a mixture of acetonitrile and 0.2% phosphoric acid (v/v) as mobile phase within 25 min. Method validation results were obtained through the generation of calibration curves with the mixed standard solutions and a standard addition method, regression equations revealed linear relationships (correlation coefficients 0.9965-0.9985) between the peak area and concentration of the three analytes. The average recoveries were 90-106% (n= 6) with standard deviation below 3.37%. The developed HPLC assay was specific and could be readily utilized for comprehensive evaluation of Shuanghuanglian preparations.
author	YANG,LI YAN,QINGHUA MO,HAIZHEN LI,XIAOMIN WANG,QING
author_facet	YANG,LI YAN,QINGHUA MO,HAIZHEN LI,XIAOMIN WANG,QING
author_sort	YANG,LI
title	DETERMINATION OF CHLOROGENIC ACID, BAICALIN AND FORSYTHIN IN SHUANGHUANGLIAN PREPARATIONS BY HPLC-DAD
title_short	DETERMINATION OF CHLOROGENIC ACID, BAICALIN AND FORSYTHIN IN SHUANGHUANGLIAN PREPARATIONS BY HPLC-DAD
title_full	DETERMINATION OF CHLOROGENIC ACID, BAICALIN AND FORSYTHIN IN SHUANGHUANGLIAN PREPARATIONS BY HPLC-DAD
title_fullStr	DETERMINATION OF CHLOROGENIC ACID, BAICALIN AND FORSYTHIN IN SHUANGHUANGLIAN PREPARATIONS BY HPLC-DAD
title_full_unstemmed	DETERMINATION OF CHLOROGENIC ACID, BAICALIN AND FORSYTHIN IN SHUANGHUANGLIAN PREPARATIONS BY HPLC-DAD
title_sort	determination of chlorogenic acid, baicalin and forsythin in shuanghuanglian preparations by hplc-dad
publisher	Sociedad Chilena de Química
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000400006
work_keys_str_mv	AT yangli determinationofchlorogenicacidbaicalinandforsythininshuanghuanglianpreparationsbyhplcdad AT yanqinghua determinationofchlorogenicacidbaicalinandforsythininshuanghuanglianpreparationsbyhplcdad AT mohaizhen determinationofchlorogenicacidbaicalinandforsythininshuanghuanglianpreparationsbyhplcdad AT lixiaomin determinationofchlorogenicacidbaicalinandforsythininshuanghuanglianpreparationsbyhplcdad AT wangqing determinationofchlorogenicacidbaicalinandforsythininshuanghuanglianpreparationsbyhplcdad
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DETERMINATION OF CHLOROGENIC ACID, BAICALIN AND FORSYTHIN IN SHUANGHUANGLIAN PREPARATIONS BY HPLC-DAD

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