SPECTROPHOTOMETRIC AND FLUORIMETRIC METHODS FOR DETERMINATION OF NALTREXONE IN URINE, SERUM AND TABLETS BY OXIDATION WITH CERIUM (IV)

This paper proposes new methods for simple and sensitive spectrophotometric and fluorimetric determination of naltrexone (NALT) in tablet and biological fluids. The spectrophotometric method involve addition of a known excess of Ce(IV) to NALT in acid medium, followed by determination of residual Ce...

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Autores principales:	EL-DIDAMONY,AKRAM M, HASSAN,WAFAA S
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2012
Materias:	Spectrophotometric fluorimetric naltrexone dosage form Ce(IV)
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000400015
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spelling	oai:scielo:S0717-970720120004000152013-04-04SPECTROPHOTOMETRIC AND FLUORIMETRIC METHODS FOR DETERMINATION OF NALTREXONE IN URINE, SERUM AND TABLETS BY OXIDATION WITH CERIUM (IV)EL-DIDAMONY,AKRAM MHASSAN,WAFAA S Spectrophotometric fluorimetric naltrexone dosage form Ce(IV) This paper proposes new methods for simple and sensitive spectrophotometric and fluorimetric determination of naltrexone (NALT) in tablet and biological fluids. The spectrophotometric method involve addition of a known excess of Ce(IV) to NALT in acid medium, followed by determination of residual Ce(IV) by reacting with a fixed amount of methyl orange, and measuring the absorbance at 510 nm. Fluorimetric method was based on the oxidation of NALT with Ce(IV) to produce Ce(III) and its fluorescence was monitored at 350 nm after excitation at 250 nm. In both methods, the amount of Ce(IV) reacted corresponds to the amount of NALT and the measured absorbance or fluorescence were found to increase linearly with the concentration of NALT, which are corroborated by the correlation coefficients of 0.9999 and 0.9996 for spectrophotometric and fluorimetric methods, respectively. Different variables affecting the reaction conditions such as the concentrations of Ce(IV), type and concentration of acid medium, reaction time, temperature, and the diluting solvents were carefully studied and optimized. The accuracy and precision of the methods were evaluated on intra-day and inter-day basis. The proposed methods were successfully applied for the determination of NALT in pharmaceutical formulation and biological samples with good recoveries.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.57 n.4 20122012-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000400015en10.4067/S0717-97072012000400015
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Spectrophotometric fluorimetric naltrexone dosage form Ce(IV)
spellingShingle	Spectrophotometric fluorimetric naltrexone dosage form Ce(IV) EL-DIDAMONY,AKRAM M HASSAN,WAFAA S SPECTROPHOTOMETRIC AND FLUORIMETRIC METHODS FOR DETERMINATION OF NALTREXONE IN URINE, SERUM AND TABLETS BY OXIDATION WITH CERIUM (IV)
description	This paper proposes new methods for simple and sensitive spectrophotometric and fluorimetric determination of naltrexone (NALT) in tablet and biological fluids. The spectrophotometric method involve addition of a known excess of Ce(IV) to NALT in acid medium, followed by determination of residual Ce(IV) by reacting with a fixed amount of methyl orange, and measuring the absorbance at 510 nm. Fluorimetric method was based on the oxidation of NALT with Ce(IV) to produce Ce(III) and its fluorescence was monitored at 350 nm after excitation at 250 nm. In both methods, the amount of Ce(IV) reacted corresponds to the amount of NALT and the measured absorbance or fluorescence were found to increase linearly with the concentration of NALT, which are corroborated by the correlation coefficients of 0.9999 and 0.9996 for spectrophotometric and fluorimetric methods, respectively. Different variables affecting the reaction conditions such as the concentrations of Ce(IV), type and concentration of acid medium, reaction time, temperature, and the diluting solvents were carefully studied and optimized. The accuracy and precision of the methods were evaluated on intra-day and inter-day basis. The proposed methods were successfully applied for the determination of NALT in pharmaceutical formulation and biological samples with good recoveries.
author	EL-DIDAMONY,AKRAM M HASSAN,WAFAA S
author_facet	EL-DIDAMONY,AKRAM M HASSAN,WAFAA S
author_sort	EL-DIDAMONY,AKRAM M
title	SPECTROPHOTOMETRIC AND FLUORIMETRIC METHODS FOR DETERMINATION OF NALTREXONE IN URINE, SERUM AND TABLETS BY OXIDATION WITH CERIUM (IV)
title_short	SPECTROPHOTOMETRIC AND FLUORIMETRIC METHODS FOR DETERMINATION OF NALTREXONE IN URINE, SERUM AND TABLETS BY OXIDATION WITH CERIUM (IV)
title_full	SPECTROPHOTOMETRIC AND FLUORIMETRIC METHODS FOR DETERMINATION OF NALTREXONE IN URINE, SERUM AND TABLETS BY OXIDATION WITH CERIUM (IV)
title_fullStr	SPECTROPHOTOMETRIC AND FLUORIMETRIC METHODS FOR DETERMINATION OF NALTREXONE IN URINE, SERUM AND TABLETS BY OXIDATION WITH CERIUM (IV)
title_full_unstemmed	SPECTROPHOTOMETRIC AND FLUORIMETRIC METHODS FOR DETERMINATION OF NALTREXONE IN URINE, SERUM AND TABLETS BY OXIDATION WITH CERIUM (IV)
title_sort	spectrophotometric and fluorimetric methods for determination of naltrexone in urine, serum and tablets by oxidation with cerium (iv)
publisher	Sociedad Chilena de Química
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000400015
work_keys_str_mv	AT eldidamonyakramm spectrophotometricandfluorimetricmethodsfordeterminationofnaltrexoneinurineserumandtabletsbyoxidationwithceriumiv AT hassanwafaas spectrophotometricandfluorimetricmethodsfordeterminationofnaltrexoneinurineserumandtabletsbyoxidationwithceriumiv
_version_	1718445475547316224

SPECTROPHOTOMETRIC AND FLUORIMETRIC METHODS FOR DETERMINATION OF NALTREXONE IN URINE, SERUM AND TABLETS BY OXIDATION WITH CERIUM (IV)

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