NOVEL SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF FLUCONAZOLE IN THE PRESENCE OF ITS OXIDATIVE DEGRADATION PRODUCT

Five sensitive, selective and precise spectrophotometric stability-indicating methods for the determination of fluconazole in the presence of its oxidative degradation product and in pharmaceutical formulations are developed and validated. Method A is a newly developed spectrophotometric one that is...

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Autores principales:	LOTFY,HAYAMMAHMOUD, MONIR,HANY HUNTER, ABD EL-ALEEM,ABD EL-AZIZEL-BAYOUMI
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2012
Materias:	Fluconazole Spectrophotometry Ratio difference spectrophotometry Stability-indicating analysis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000400023
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spelling	oai:scielo:S0717-970720120004000232013-04-04NOVEL SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF FLUCONAZOLE IN THE PRESENCE OF ITS OXIDATIVE DEGRADATION PRODUCTLOTFY,HAYAMMAHMOUDMONIR,HANY HUNTERABD EL-ALEEM,ABD EL-AZIZEL-BAYOUMI Fluconazole Spectrophotometry Ratio difference spectrophotometry Stability-indicating analysis Five sensitive, selective and precise spectrophotometric stability-indicating methods for the determination of fluconazole in the presence of its oxidative degradation product and in pharmaceutical formulations are developed and validated. Method A is a newly developed spectrophotometric one that is called ratio difference method by measuring the difference in amplitudes between 261.5 and 266.5 nm of ratio spectrum. Method B is a third derivative spectropho-tometric one, which determines peak amplitudes from 265.5-268nm (peak-peak). Method C is ratio subtraction method. Method D is the first derivative of the ratio spectra by measuring the peak amplitude at 269 nm. Finally, method E is the mean centering of ratio spectra method by measuring the peak amplitude at 266.5 nm. The proposed methods allow the determination of fluconazole in the presence of its oxidative degradation product over a concentration range of 50400 µg.mL-1 with mean percentage recoveries of 100.24±0.91, 99.91±0.52, 100.20±1.26, 99.79±0.69, and 100.77±0.72 respectively. The selectivity of the proposed methods is checked using laboratory prepared mixtures. The proposed methods have been successfully applied to the analysis of fluconazole in pharmaceutical dosage forms without interference from other dosage form additives and the results have been statistically compared with a reported method.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.57 n.4 20122012-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000400023en10.4067/S0717-97072012000400023
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Fluconazole Spectrophotometry Ratio difference spectrophotometry Stability-indicating analysis
spellingShingle	Fluconazole Spectrophotometry Ratio difference spectrophotometry Stability-indicating analysis LOTFY,HAYAMMAHMOUD MONIR,HANY HUNTER ABD EL-ALEEM,ABD EL-AZIZEL-BAYOUMI NOVEL SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF FLUCONAZOLE IN THE PRESENCE OF ITS OXIDATIVE DEGRADATION PRODUCT
description	Five sensitive, selective and precise spectrophotometric stability-indicating methods for the determination of fluconazole in the presence of its oxidative degradation product and in pharmaceutical formulations are developed and validated. Method A is a newly developed spectrophotometric one that is called ratio difference method by measuring the difference in amplitudes between 261.5 and 266.5 nm of ratio spectrum. Method B is a third derivative spectropho-tometric one, which determines peak amplitudes from 265.5-268nm (peak-peak). Method C is ratio subtraction method. Method D is the first derivative of the ratio spectra by measuring the peak amplitude at 269 nm. Finally, method E is the mean centering of ratio spectra method by measuring the peak amplitude at 266.5 nm. The proposed methods allow the determination of fluconazole in the presence of its oxidative degradation product over a concentration range of 50400 µg.mL-1 with mean percentage recoveries of 100.24±0.91, 99.91±0.52, 100.20±1.26, 99.79±0.69, and 100.77±0.72 respectively. The selectivity of the proposed methods is checked using laboratory prepared mixtures. The proposed methods have been successfully applied to the analysis of fluconazole in pharmaceutical dosage forms without interference from other dosage form additives and the results have been statistically compared with a reported method.
author	LOTFY,HAYAMMAHMOUD MONIR,HANY HUNTER ABD EL-ALEEM,ABD EL-AZIZEL-BAYOUMI
author_facet	LOTFY,HAYAMMAHMOUD MONIR,HANY HUNTER ABD EL-ALEEM,ABD EL-AZIZEL-BAYOUMI
author_sort	LOTFY,HAYAMMAHMOUD
title	NOVEL SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF FLUCONAZOLE IN THE PRESENCE OF ITS OXIDATIVE DEGRADATION PRODUCT
title_short	NOVEL SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF FLUCONAZOLE IN THE PRESENCE OF ITS OXIDATIVE DEGRADATION PRODUCT
title_full	NOVEL SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF FLUCONAZOLE IN THE PRESENCE OF ITS OXIDATIVE DEGRADATION PRODUCT
title_fullStr	NOVEL SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF FLUCONAZOLE IN THE PRESENCE OF ITS OXIDATIVE DEGRADATION PRODUCT
title_full_unstemmed	NOVEL SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF FLUCONAZOLE IN THE PRESENCE OF ITS OXIDATIVE DEGRADATION PRODUCT
title_sort	novel spectrophotometric methods for the determination of fluconazole in the presence of its oxidative degradation product
publisher	Sociedad Chilena de Química
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000400023
work_keys_str_mv	AT lotfyhayammahmoud novelspectrophotometricmethodsforthedeterminationoffluconazoleinthepresenceofitsoxidativedegradationproduct AT monirhanyhunter novelspectrophotometricmethodsforthedeterminationoffluconazoleinthepresenceofitsoxidativedegradationproduct AT abdelaleemabdelazizelbayoumi novelspectrophotometricmethodsforthedeterminationoffluconazoleinthepresenceofitsoxidativedegradationproduct
_version_	1718445477023711232

NOVEL SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF FLUCONAZOLE IN THE PRESENCE OF ITS OXIDATIVE DEGRADATION PRODUCT

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