DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHIC METHOD FOR NAPROXEN AND ESOMEPRAZOLE IN BINARY COMBINATION

The present study reports the development and validation of a simple, economic and sensitive HPLC method for the concurrent determination of naproxen and esomeprazole in pharmaceutical formulations. Isocratic chromatography was performed with C-18 column and mixture of phosphate buffer (pH 6.1) and...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	RAZZAQ,SYED NAEEM, ASHFAQ,MUHAMMAD, KHAN,ISLAM ULLAH, MARIAM,IRFANA
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2012
Materias:	HPLC acetonitrile isocratic specificity forced degradation study
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000400024
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-97072012000400024
record_format	dspace
spelling	oai:scielo:S0717-970720120004000242013-04-04DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHIC METHOD FOR NAPROXEN AND ESOMEPRAZOLE IN BINARY COMBINATIONRAZZAQ,SYED NAEEMASHFAQ,MUHAMMADKHAN,ISLAM ULLAHMARIAM,IRFANA HPLC acetonitrile isocratic specificity forced degradation study The present study reports the development and validation of a simple, economic and sensitive HPLC method for the concurrent determination of naproxen and esomeprazole in pharmaceutical formulations. Isocratic chromatography was performed with C-18 column and mixture of phosphate buffer (pH 6.1) and acetonitrile in ratio of (40:60, v/v) at 1.5 mlmin-1. The eluents were monitored at 302 nm using UV detector. The method was isocratic in the range of 9.38 to 300 µgml-1 for naproxen and 0.5 to 16 µgml-1 for esomeprazole. Validation of the method was performed by testing parameters like linearity, accuracy, precision, robustness, specificity, LOD and LOQ values. In the specificity the drugs were subjected to forced degradation studies like acidic, basic, oxidative and thermal stresses. Both the analytes were separated within three minutes. As the method separates the degradation products produced during forced degradation studies from the active analytes so it can be used not only for regular determination of naproxen and esomeprazole but also for their stability studies.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.57 n.4 20122012-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000400024en10.4067/S0717-97072012000400024
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	HPLC acetonitrile isocratic specificity forced degradation study
spellingShingle	HPLC acetonitrile isocratic specificity forced degradation study RAZZAQ,SYED NAEEM ASHFAQ,MUHAMMAD KHAN,ISLAM ULLAH MARIAM,IRFANA DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHIC METHOD FOR NAPROXEN AND ESOMEPRAZOLE IN BINARY COMBINATION
description	The present study reports the development and validation of a simple, economic and sensitive HPLC method for the concurrent determination of naproxen and esomeprazole in pharmaceutical formulations. Isocratic chromatography was performed with C-18 column and mixture of phosphate buffer (pH 6.1) and acetonitrile in ratio of (40:60, v/v) at 1.5 mlmin-1. The eluents were monitored at 302 nm using UV detector. The method was isocratic in the range of 9.38 to 300 µgml-1 for naproxen and 0.5 to 16 µgml-1 for esomeprazole. Validation of the method was performed by testing parameters like linearity, accuracy, precision, robustness, specificity, LOD and LOQ values. In the specificity the drugs were subjected to forced degradation studies like acidic, basic, oxidative and thermal stresses. Both the analytes were separated within three minutes. As the method separates the degradation products produced during forced degradation studies from the active analytes so it can be used not only for regular determination of naproxen and esomeprazole but also for their stability studies.
author	RAZZAQ,SYED NAEEM ASHFAQ,MUHAMMAD KHAN,ISLAM ULLAH MARIAM,IRFANA
author_facet	RAZZAQ,SYED NAEEM ASHFAQ,MUHAMMAD KHAN,ISLAM ULLAH MARIAM,IRFANA
author_sort	RAZZAQ,SYED NAEEM
title	DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHIC METHOD FOR NAPROXEN AND ESOMEPRAZOLE IN BINARY COMBINATION
title_short	DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHIC METHOD FOR NAPROXEN AND ESOMEPRAZOLE IN BINARY COMBINATION
title_full	DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHIC METHOD FOR NAPROXEN AND ESOMEPRAZOLE IN BINARY COMBINATION
title_fullStr	DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHIC METHOD FOR NAPROXEN AND ESOMEPRAZOLE IN BINARY COMBINATION
title_full_unstemmed	DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHIC METHOD FOR NAPROXEN AND ESOMEPRAZOLE IN BINARY COMBINATION
title_sort	development and validation of liquid chromatographic method for naproxen and esomeprazole in binary combination
publisher	Sociedad Chilena de Química
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000400024
work_keys_str_mv	AT razzaqsyednaeem developmentandvalidationofliquidchromatographicmethodfornaproxenandesomeprazoleinbinarycombination AT ashfaqmuhammad developmentandvalidationofliquidchromatographicmethodfornaproxenandesomeprazoleinbinarycombination AT khanislamullah developmentandvalidationofliquidchromatographicmethodfornaproxenandesomeprazoleinbinarycombination AT mariamirfana developmentandvalidationofliquidchromatographicmethodfornaproxenandesomeprazoleinbinarycombination
_version_	1718445477201969152

DEVELOPMENT AND VALIDATION OF LIQUID CHROMATOGRAPHIC METHOD FOR NAPROXEN AND ESOMEPRAZOLE IN BINARY COMBINATION

Ejemplares similares