RAPID ANALYSIS OF FINASTERIDE IN BULK AND FORMULATIONS BY RP-HPLC-PDA METHOD

A simple, precise, rapid and accurate LC-MS compatible RP-HPLC-PDA method has been developed and validated for the estimation of Finasteride (FIN) in bulk and tablet formulations. The chromatographic separation was achieved on Phenomenex C18 column (150 mm x 4.6 mm, 5.0 µ particle size) using the mo...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	MANNE,SINDHURA, KAKARLA,RAGHAVI, IRAAVI,PRASHANTH, NALLURI,BUCHI N
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2012
Materias:	Rapid Finasteride HPLC validation LC-MS compatible Phenomenex C18 column 220nm
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000400027
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-97072012000400027
record_format	dspace
spelling	oai:scielo:S0717-970720120004000272013-04-04RAPID ANALYSIS OF FINASTERIDE IN BULK AND FORMULATIONS BY RP-HPLC-PDA METHODMANNE,SINDHURAKAKARLA,RAGHAVIIRAAVI,PRASHANTHNALLURI,BUCHI N Rapid Finasteride HPLC validation LC-MS compatible Phenomenex C18 column 220nm A simple, precise, rapid and accurate LC-MS compatible RP-HPLC-PDA method has been developed and validated for the estimation of Finasteride (FIN) in bulk and tablet formulations. The chromatographic separation was achieved on Phenomenex C18 column (150 mm x 4.6 mm, 5.0 µ particle size) using the mobile phase comprising 0.02% formic acid (in water): methanol in the ratio of 20:80 (v/v) at a flow rate of 1mL/min and FIN was eluted at 3.4 min. Quantification and linearity were achieved at 220 nm over the concentration range of 5-50 µg/mL and the percentage mean of assay was found to be 100.29. The method was validated for specificity, linearity, accuracy, precision, LOD, LOQ, stability and robustness as per the ICH guidelines and is suitable to be employed in Quality Control.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.57 n.4 20122012-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000400027en10.4067/S0717-97072012000400027
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Rapid Finasteride HPLC validation LC-MS compatible Phenomenex C18 column 220nm
spellingShingle	Rapid Finasteride HPLC validation LC-MS compatible Phenomenex C18 column 220nm MANNE,SINDHURA KAKARLA,RAGHAVI IRAAVI,PRASHANTH NALLURI,BUCHI N RAPID ANALYSIS OF FINASTERIDE IN BULK AND FORMULATIONS BY RP-HPLC-PDA METHOD
description	A simple, precise, rapid and accurate LC-MS compatible RP-HPLC-PDA method has been developed and validated for the estimation of Finasteride (FIN) in bulk and tablet formulations. The chromatographic separation was achieved on Phenomenex C18 column (150 mm x 4.6 mm, 5.0 µ particle size) using the mobile phase comprising 0.02% formic acid (in water): methanol in the ratio of 20:80 (v/v) at a flow rate of 1mL/min and FIN was eluted at 3.4 min. Quantification and linearity were achieved at 220 nm over the concentration range of 5-50 µg/mL and the percentage mean of assay was found to be 100.29. The method was validated for specificity, linearity, accuracy, precision, LOD, LOQ, stability and robustness as per the ICH guidelines and is suitable to be employed in Quality Control.
author	MANNE,SINDHURA KAKARLA,RAGHAVI IRAAVI,PRASHANTH NALLURI,BUCHI N
author_facet	MANNE,SINDHURA KAKARLA,RAGHAVI IRAAVI,PRASHANTH NALLURI,BUCHI N
author_sort	MANNE,SINDHURA
title	RAPID ANALYSIS OF FINASTERIDE IN BULK AND FORMULATIONS BY RP-HPLC-PDA METHOD
title_short	RAPID ANALYSIS OF FINASTERIDE IN BULK AND FORMULATIONS BY RP-HPLC-PDA METHOD
title_full	RAPID ANALYSIS OF FINASTERIDE IN BULK AND FORMULATIONS BY RP-HPLC-PDA METHOD
title_fullStr	RAPID ANALYSIS OF FINASTERIDE IN BULK AND FORMULATIONS BY RP-HPLC-PDA METHOD
title_full_unstemmed	RAPID ANALYSIS OF FINASTERIDE IN BULK AND FORMULATIONS BY RP-HPLC-PDA METHOD
title_sort	rapid analysis of finasteride in bulk and formulations by rp-hplc-pda method
publisher	Sociedad Chilena de Química
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072012000400027
work_keys_str_mv	AT mannesindhura rapidanalysisoffinasterideinbulkandformulationsbyrphplcpdamethod AT kakarlaraghavi rapidanalysisoffinasterideinbulkandformulationsbyrphplcpdamethod AT iraaviprashanth rapidanalysisoffinasterideinbulkandformulationsbyrphplcpdamethod AT nalluribuchin rapidanalysisoffinasterideinbulkandformulationsbyrphplcpdamethod
_version_	1718445477697945600

RAPID ANALYSIS OF FINASTERIDE IN BULK AND FORMULATIONS BY RP-HPLC-PDA METHOD

Ejemplares similares