ZERO-CROSSING DERIVATIVE SPECTROPHOTOMETRY AND LIQUID CHROMATOGRAPHIC METHOD FOR THE QUANTITATIVE DETERMINATION OF CLOPIDOGREL IN PRESENCE OF HMG CO-A REDUCTASE INHIBITORS

In this work, two methods are described for the simultaneous determination of clopidogrel bisulfate with HMG Co-A reductase inhibitors/statins. The first method comprised of quantitative determinations of clopidogrel bisulfate in presence of HMG Co-A reductase inhibitors (atorvastatin, pravastatin s...

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Autores principales: SULTANA,NAJMA, ARAYNE,MUHAMMAD SAEED, NAWAZ,MUHAMMAD, AMIR ALI,KIRAN
Lenguaje:English
Publicado: Sociedad Chilena de Química 2013
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072013000100018
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spelling oai:scielo:S0717-970720130001000182014-09-09ZERO-CROSSING DERIVATIVE SPECTROPHOTOMETRY AND LIQUID CHROMATOGRAPHIC METHOD FOR THE QUANTITATIVE DETERMINATION OF CLOPIDOGREL IN PRESENCE OF HMG CO-A REDUCTASE INHIBITORSSULTANA,NAJMAARAYNE,MUHAMMAD SAEEDNAWAZ,MUHAMMADAMIR ALI,KIRAN Clopidogrel Statins Interactions UV-Vis RP-HPLC In this work, two methods are described for the simultaneous determination of clopidogrel bisulfate with HMG Co-A reductase inhibitors/statins. The first method comprised of quantitative determinations of clopidogrel bisulfate in presence of HMG Co-A reductase inhibitors (atorvastatin, pravastatin simvastatin and rosuvastatin) in buffers of pH 1, 4 (simulating gastric environments), pH 7.4 (simulating blood pH) and pH 9 (simulating intestinal pH) at body temperature (37ºC) using UV/VIS spectrophotometer. Derivative spectroscopy coupled with zero-crossing measurements was used for separating the interfering wavelengths of clopidogrel and statins. Least square regression analysis was performed to measure the concentrations of these drugs. It was observed that clopidogrel interacted with statins at all four pH, especially in simulated gastric environments. In the second case, reversed phase liquid chromatographic (RP-LC) method was developed for simultaneous determination and to investigate the interactions between clopidogrel and HMG Co-A reductase inhibitors. These interactions were evaluated by RP-LC in buffers of pH 1, 4, 7.4 and 9. These studies further supported the evidence of complexation between clopidogrel and statins as the area under the curve (AUC) was found increased in almost all four pH solutions.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.58 n.1 20132013-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072013000100018en10.4067/S0717-97072013000100018
institution Scielo Chile
collection Scielo Chile
language English
topic Clopidogrel
Statins
Interactions
UV-Vis
RP-HPLC
spellingShingle Clopidogrel
Statins
Interactions
UV-Vis
RP-HPLC
SULTANA,NAJMA
ARAYNE,MUHAMMAD SAEED
NAWAZ,MUHAMMAD
AMIR ALI,KIRAN
ZERO-CROSSING DERIVATIVE SPECTROPHOTOMETRY AND LIQUID CHROMATOGRAPHIC METHOD FOR THE QUANTITATIVE DETERMINATION OF CLOPIDOGREL IN PRESENCE OF HMG CO-A REDUCTASE INHIBITORS
description In this work, two methods are described for the simultaneous determination of clopidogrel bisulfate with HMG Co-A reductase inhibitors/statins. The first method comprised of quantitative determinations of clopidogrel bisulfate in presence of HMG Co-A reductase inhibitors (atorvastatin, pravastatin simvastatin and rosuvastatin) in buffers of pH 1, 4 (simulating gastric environments), pH 7.4 (simulating blood pH) and pH 9 (simulating intestinal pH) at body temperature (37ºC) using UV/VIS spectrophotometer. Derivative spectroscopy coupled with zero-crossing measurements was used for separating the interfering wavelengths of clopidogrel and statins. Least square regression analysis was performed to measure the concentrations of these drugs. It was observed that clopidogrel interacted with statins at all four pH, especially in simulated gastric environments. In the second case, reversed phase liquid chromatographic (RP-LC) method was developed for simultaneous determination and to investigate the interactions between clopidogrel and HMG Co-A reductase inhibitors. These interactions were evaluated by RP-LC in buffers of pH 1, 4, 7.4 and 9. These studies further supported the evidence of complexation between clopidogrel and statins as the area under the curve (AUC) was found increased in almost all four pH solutions.
author SULTANA,NAJMA
ARAYNE,MUHAMMAD SAEED
NAWAZ,MUHAMMAD
AMIR ALI,KIRAN
author_facet SULTANA,NAJMA
ARAYNE,MUHAMMAD SAEED
NAWAZ,MUHAMMAD
AMIR ALI,KIRAN
author_sort SULTANA,NAJMA
title ZERO-CROSSING DERIVATIVE SPECTROPHOTOMETRY AND LIQUID CHROMATOGRAPHIC METHOD FOR THE QUANTITATIVE DETERMINATION OF CLOPIDOGREL IN PRESENCE OF HMG CO-A REDUCTASE INHIBITORS
title_short ZERO-CROSSING DERIVATIVE SPECTROPHOTOMETRY AND LIQUID CHROMATOGRAPHIC METHOD FOR THE QUANTITATIVE DETERMINATION OF CLOPIDOGREL IN PRESENCE OF HMG CO-A REDUCTASE INHIBITORS
title_full ZERO-CROSSING DERIVATIVE SPECTROPHOTOMETRY AND LIQUID CHROMATOGRAPHIC METHOD FOR THE QUANTITATIVE DETERMINATION OF CLOPIDOGREL IN PRESENCE OF HMG CO-A REDUCTASE INHIBITORS
title_fullStr ZERO-CROSSING DERIVATIVE SPECTROPHOTOMETRY AND LIQUID CHROMATOGRAPHIC METHOD FOR THE QUANTITATIVE DETERMINATION OF CLOPIDOGREL IN PRESENCE OF HMG CO-A REDUCTASE INHIBITORS
title_full_unstemmed ZERO-CROSSING DERIVATIVE SPECTROPHOTOMETRY AND LIQUID CHROMATOGRAPHIC METHOD FOR THE QUANTITATIVE DETERMINATION OF CLOPIDOGREL IN PRESENCE OF HMG CO-A REDUCTASE INHIBITORS
title_sort zero-crossing derivative spectrophotometry and liquid chromatographic method for the quantitative determination of clopidogrel in presence of hmg co-a reductase inhibitors
publisher Sociedad Chilena de Química
publishDate 2013
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072013000100018
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