HIGH SPEED CENTRIFUGAL COUNTERCURRENT CHROMATOGRAPHY (HSCCC) ISOLATION AND IDENTIFICATION BY LC-MSn ANALYSIS OF THE POLAR PHENOLICS FROM VASCONCELLEA QUERCIFOLIA

High speed centrifugal countercurrent chromatography (HSCCC) was successfully applied to preparative isolation of the main polar phenolics from the Peruvian Caricaceae Vasconcellea quercifolia A. St.-Hil. High-performance liquid chromatography with diode array (HPLC-DAD) and electrospray ionisation...

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Autores principales:	QUISPE,CRISTINA, VIVEROS-VALDEZ,EZEQUIEL, YARLEQUE,JOSÉ A, ARONES,MARCO R, PANIAGUA,JUAN C, SCHMEDA-HIRSCHMANN,GUILLERMO
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2013
Materias:	Vasconcellea quercifolia Caricaceae Flavonoids High-speed countercurrent chromatography High-pressure liquid chromatography
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072013000300007
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spelling	oai:scielo:S0717-970720130003000072014-09-09HIGH SPEED CENTRIFUGAL COUNTERCURRENT CHROMATOGRAPHY (HSCCC) ISOLATION AND IDENTIFICATION BY LC-MSn ANALYSIS OF THE POLAR PHENOLICS FROM VASCONCELLEA QUERCIFOLIAQUISPE,CRISTINAVIVEROS-VALDEZ,EZEQUIELYARLEQUE,JOSÉ AARONES,MARCO RPANIAGUA,JUAN CSCHMEDA-HIRSCHMANN,GUILLERMO Vasconcellea quercifolia Caricaceae Flavonoids High-speed countercurrent chromatography High-pressure liquid chromatography High speed centrifugal countercurrent chromatography (HSCCC) was successfully applied to preparative isolation of the main polar phenolics from the Peruvian Caricaceae Vasconcellea quercifolia A. St.-Hil. High-performance liquid chromatography with diode array (HPLC-DAD) and electrospray ionisation mass spectrometric detection (ESI-MS) was used to carry out a comprehensive characterisation of phenolic compounds from fruits and leaves of this plant. The main phenolics were the quercetin diglycoside rutin and the triglycoside manghaslin. Nine minor flavonoids were tentatively identified as kaempferol, quercetin, isorhamnetin and methoxymyricetin glycosides. HSCCC combined with other chromatographic methods allowed the isolation of the main phenolics from the plant. HPLC-DAD-MS analysis shows that the main phenolic compounds in V. quercifolia are similar to those described for V. pubescens but differs in the identity of the minor constituents.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.58 n.3 20132013-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072013000300007en10.4067/S0717-97072013000300007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Vasconcellea quercifolia Caricaceae Flavonoids High-speed countercurrent chromatography High-pressure liquid chromatography
spellingShingle	Vasconcellea quercifolia Caricaceae Flavonoids High-speed countercurrent chromatography High-pressure liquid chromatography QUISPE,CRISTINA VIVEROS-VALDEZ,EZEQUIEL YARLEQUE,JOSÉ A ARONES,MARCO R PANIAGUA,JUAN C SCHMEDA-HIRSCHMANN,GUILLERMO HIGH SPEED CENTRIFUGAL COUNTERCURRENT CHROMATOGRAPHY (HSCCC) ISOLATION AND IDENTIFICATION BY LC-MSn ANALYSIS OF THE POLAR PHENOLICS FROM VASCONCELLEA QUERCIFOLIA
description	High speed centrifugal countercurrent chromatography (HSCCC) was successfully applied to preparative isolation of the main polar phenolics from the Peruvian Caricaceae Vasconcellea quercifolia A. St.-Hil. High-performance liquid chromatography with diode array (HPLC-DAD) and electrospray ionisation mass spectrometric detection (ESI-MS) was used to carry out a comprehensive characterisation of phenolic compounds from fruits and leaves of this plant. The main phenolics were the quercetin diglycoside rutin and the triglycoside manghaslin. Nine minor flavonoids were tentatively identified as kaempferol, quercetin, isorhamnetin and methoxymyricetin glycosides. HSCCC combined with other chromatographic methods allowed the isolation of the main phenolics from the plant. HPLC-DAD-MS analysis shows that the main phenolic compounds in V. quercifolia are similar to those described for V. pubescens but differs in the identity of the minor constituents.
author	QUISPE,CRISTINA VIVEROS-VALDEZ,EZEQUIEL YARLEQUE,JOSÉ A ARONES,MARCO R PANIAGUA,JUAN C SCHMEDA-HIRSCHMANN,GUILLERMO
author_facet	QUISPE,CRISTINA VIVEROS-VALDEZ,EZEQUIEL YARLEQUE,JOSÉ A ARONES,MARCO R PANIAGUA,JUAN C SCHMEDA-HIRSCHMANN,GUILLERMO
author_sort	QUISPE,CRISTINA
title	HIGH SPEED CENTRIFUGAL COUNTERCURRENT CHROMATOGRAPHY (HSCCC) ISOLATION AND IDENTIFICATION BY LC-MSn ANALYSIS OF THE POLAR PHENOLICS FROM VASCONCELLEA QUERCIFOLIA
title_short	HIGH SPEED CENTRIFUGAL COUNTERCURRENT CHROMATOGRAPHY (HSCCC) ISOLATION AND IDENTIFICATION BY LC-MSn ANALYSIS OF THE POLAR PHENOLICS FROM VASCONCELLEA QUERCIFOLIA
title_full	HIGH SPEED CENTRIFUGAL COUNTERCURRENT CHROMATOGRAPHY (HSCCC) ISOLATION AND IDENTIFICATION BY LC-MSn ANALYSIS OF THE POLAR PHENOLICS FROM VASCONCELLEA QUERCIFOLIA
title_fullStr	HIGH SPEED CENTRIFUGAL COUNTERCURRENT CHROMATOGRAPHY (HSCCC) ISOLATION AND IDENTIFICATION BY LC-MSn ANALYSIS OF THE POLAR PHENOLICS FROM VASCONCELLEA QUERCIFOLIA
title_full_unstemmed	HIGH SPEED CENTRIFUGAL COUNTERCURRENT CHROMATOGRAPHY (HSCCC) ISOLATION AND IDENTIFICATION BY LC-MSn ANALYSIS OF THE POLAR PHENOLICS FROM VASCONCELLEA QUERCIFOLIA
title_sort	high speed centrifugal countercurrent chromatography (hsccc) isolation and identification by lc-msn analysis of the polar phenolics from vasconcellea quercifolia
publisher	Sociedad Chilena de Química
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072013000300007
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HIGH SPEED CENTRIFUGAL COUNTERCURRENT CHROMATOGRAPHY (HSCCC) ISOLATION AND IDENTIFICATION BY LC-MSn ANALYSIS OF THE POLAR PHENOLICS FROM VASCONCELLEA QUERCIFOLIA

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