COVALENT IMMOBILIZATION OF GLUCOSE OXIDASE ON WELL-DEFINED POLYMER-SILICON WAFER HYBRIDS VIA SURFACE-INITIATED RAFT-MEDIATED PROCESS

COVALENT IMMOBILIZATION OF GLUCOSE OXIDASE ON WELL-DEFINED POLYMER-SILICON WAFER HYBRIDS VIA SURFACE-INITIATED RAFT-MEDIATED PROCESS

A surface modification technique was developed for the functionalization of silicon surface with glucose oxidase (GOD). The silicon surface was first graft copolymerized with glycidyl methacrylate (GMA) via surface-initiated reversible addition-fragmentation chain-transfer (RAFT)-mediated process. G...

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Autores principales: JI,JIAYOU, XIA,KEQIANG, LIU,TINGTING, LI,LIANG
Lenguaje:English
Publicado: Sociedad Chilena de Química 2013
Materias:
surface-initiated RAFT
silicon
poly(glycidyl methacrylate)
glucose oxidase
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072013000300017
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spelling oai:scielo:S0717-970720130003000172014-09-09COVALENT IMMOBILIZATION OF GLUCOSE OXIDASE ON WELL-DEFINED POLYMER-SILICON WAFER HYBRIDS VIA SURFACE-INITIATED RAFT-MEDIATED PROCESSJI,JIAYOUXIA,KEQIANGLIU,TINGTINGLI,LIANG surface-initiated RAFT silicon poly(glycidyl methacrylate) glucose oxidase A surface modification technique was developed for the functionalization of silicon surface with glucose oxidase (GOD). The silicon surface was first graft copolymerized with glycidyl methacrylate (GMA) via surface-initiated reversible addition-fragmentation chain-transfer (RAFT)-mediated process. GOD was then covalently immobilized through the ring-opening reaction between the amine groups of the GOD and the epoxide groups of the grafted GMA polymer chains. X-ray photoelectron spectroscopy (XPS) was used to characterize the surface-modified surface after each modification stage. Increasing the thickness of the polymer layer and the immobilization time could allow a great amount of GOD to be immobilized on the silicon surface. The GOD-functionalized silicon hybrids are promising candidates for the silicon-based glucose biosensors.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.58 n.3 20132013-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072013000300017en10.4067/S0717-97072013000300017
institution Scielo Chile
collection Scielo Chile
language English
topic surface-initiated RAFT
silicon
poly(glycidyl methacrylate)
glucose oxidase
spellingShingle surface-initiated RAFT
silicon
poly(glycidyl methacrylate)
glucose oxidase
JI,JIAYOU
XIA,KEQIANG
LIU,TINGTING
LI,LIANG
COVALENT IMMOBILIZATION OF GLUCOSE OXIDASE ON WELL-DEFINED POLYMER-SILICON WAFER HYBRIDS VIA SURFACE-INITIATED RAFT-MEDIATED PROCESS
description A surface modification technique was developed for the functionalization of silicon surface with glucose oxidase (GOD). The silicon surface was first graft copolymerized with glycidyl methacrylate (GMA) via surface-initiated reversible addition-fragmentation chain-transfer (RAFT)-mediated process. GOD was then covalently immobilized through the ring-opening reaction between the amine groups of the GOD and the epoxide groups of the grafted GMA polymer chains. X-ray photoelectron spectroscopy (XPS) was used to characterize the surface-modified surface after each modification stage. Increasing the thickness of the polymer layer and the immobilization time could allow a great amount of GOD to be immobilized on the silicon surface. The GOD-functionalized silicon hybrids are promising candidates for the silicon-based glucose biosensors.
author JI,JIAYOU
XIA,KEQIANG
LIU,TINGTING
LI,LIANG
author_facet JI,JIAYOU
XIA,KEQIANG
LIU,TINGTING
LI,LIANG
author_sort JI,JIAYOU
title COVALENT IMMOBILIZATION OF GLUCOSE OXIDASE ON WELL-DEFINED POLYMER-SILICON WAFER HYBRIDS VIA SURFACE-INITIATED RAFT-MEDIATED PROCESS
title_short COVALENT IMMOBILIZATION OF GLUCOSE OXIDASE ON WELL-DEFINED POLYMER-SILICON WAFER HYBRIDS VIA SURFACE-INITIATED RAFT-MEDIATED PROCESS
title_full COVALENT IMMOBILIZATION OF GLUCOSE OXIDASE ON WELL-DEFINED POLYMER-SILICON WAFER HYBRIDS VIA SURFACE-INITIATED RAFT-MEDIATED PROCESS
title_fullStr COVALENT IMMOBILIZATION OF GLUCOSE OXIDASE ON WELL-DEFINED POLYMER-SILICON WAFER HYBRIDS VIA SURFACE-INITIATED RAFT-MEDIATED PROCESS
title_full_unstemmed COVALENT IMMOBILIZATION OF GLUCOSE OXIDASE ON WELL-DEFINED POLYMER-SILICON WAFER HYBRIDS VIA SURFACE-INITIATED RAFT-MEDIATED PROCESS
title_sort covalent immobilization of glucose oxidase on well-defined polymer-silicon wafer hybrids via surface-initiated raft-mediated process
publisher Sociedad Chilena de Química
publishDate 2013
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072013000300017
work_keys_str_mv AT jijiayou covalentimmobilizationofglucoseoxidaseonwelldefinedpolymersiliconwaferhybridsviasurfaceinitiatedraftmediatedprocess
AT xiakeqiang covalentimmobilizationofglucoseoxidaseonwelldefinedpolymersiliconwaferhybridsviasurfaceinitiatedraftmediatedprocess
AT liutingting covalentimmobilizationofglucoseoxidaseonwelldefinedpolymersiliconwaferhybridsviasurfaceinitiatedraftmediatedprocess
AT liliang covalentimmobilizationofglucoseoxidaseonwelldefinedpolymersiliconwaferhybridsviasurfaceinitiatedraftmediatedprocess
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