COMPARATIVE STUDY OF NEW SPECTROPHOTOMETRIC METHODS; AN APPLICATION ON PHARMACEUTICAL BINARY MIXTURE OF CIPROFLOXACIN HYDROCHLORIDE AND HYDROCORTISONE

Simple, specific, accurate and precise spectrophotometric methods were developed and validated for simultaneous estimation of Ciprofloxacin hydrochloride (CIP) and Hydrocortisone (HYD) in their pure form and pharmaceutical dosage form. Two new spectrophotometric methods were applied: extended ratio...

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Autores principales:	LOTFY,HAYAM M, HASSAN,NAGIBA Y, ELGIZAWY,SAMIA M, SALEH,SARAH S
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2013
Materias:	Ciprofloxacin Hydrocortisone extended ratio subtraction ratio difference mean centering isoabsorpative point
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072013000300022
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spelling	oai:scielo:S0717-970720130003000222014-09-09COMPARATIVE STUDY OF NEW SPECTROPHOTOMETRIC METHODS; AN APPLICATION ON PHARMACEUTICAL BINARY MIXTURE OF CIPROFLOXACIN HYDROCHLORIDE AND HYDROCORTISONELOTFY,HAYAM MHASSAN,NAGIBA YELGIZAWY,SAMIA MSALEH,SARAH S Ciprofloxacin Hydrocortisone extended ratio subtraction ratio difference mean centering isoabsorpative point Simple, specific, accurate and precise spectrophotometric methods were developed and validated for simultaneous estimation of Ciprofloxacin hydrochloride (CIP) and Hydrocortisone (HYD) in their pure form and pharmaceutical dosage form. Two new spectrophotometric methods were applied: extended ratio subtraction (EXRSM) and ratio difference (RDSM) methods. The results were compared to three well-established methods: Mean centering of ratio spectra method (MCR), Isoabsorpative point spectrophotometric method (IsoM) and Absorbance ratio method (ARM). The linearity range for the spectrophotometric methods was found to be (2-14 μg/mL) and (1-14 μg/mL) for CIP and HYD respectively. The selectivity of the developed methods was investigated by analyzing laboratory prepared mixtures of the two drugs and their combined dosage form. The results obtained from the proposed methods were statistically compared using one-way analysis of variance (ANOVA) where no significant difference was observed between the new (EXRSM and RDSM) and the well-established methods which prove their validity for the analysis of this binary mixture. The methods were validated as per ICH guidelines regarding accuracy, precision, repeatability and robustness; which were found to be within the acceptable limits. The new methods were simple, sensitive and don't need a special program, so they could be easily applied as alternative methods to LC methods in quality control laboratories lacking the required facilities for those techniques.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.58 n.3 20132013-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072013000300022en10.4067/S0717-97072013000300022
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Ciprofloxacin Hydrocortisone extended ratio subtraction ratio difference mean centering isoabsorpative point
spellingShingle	Ciprofloxacin Hydrocortisone extended ratio subtraction ratio difference mean centering isoabsorpative point LOTFY,HAYAM M HASSAN,NAGIBA Y ELGIZAWY,SAMIA M SALEH,SARAH S COMPARATIVE STUDY OF NEW SPECTROPHOTOMETRIC METHODS; AN APPLICATION ON PHARMACEUTICAL BINARY MIXTURE OF CIPROFLOXACIN HYDROCHLORIDE AND HYDROCORTISONE
description	Simple, specific, accurate and precise spectrophotometric methods were developed and validated for simultaneous estimation of Ciprofloxacin hydrochloride (CIP) and Hydrocortisone (HYD) in their pure form and pharmaceutical dosage form. Two new spectrophotometric methods were applied: extended ratio subtraction (EXRSM) and ratio difference (RDSM) methods. The results were compared to three well-established methods: Mean centering of ratio spectra method (MCR), Isoabsorpative point spectrophotometric method (IsoM) and Absorbance ratio method (ARM). The linearity range for the spectrophotometric methods was found to be (2-14 μg/mL) and (1-14 μg/mL) for CIP and HYD respectively. The selectivity of the developed methods was investigated by analyzing laboratory prepared mixtures of the two drugs and their combined dosage form. The results obtained from the proposed methods were statistically compared using one-way analysis of variance (ANOVA) where no significant difference was observed between the new (EXRSM and RDSM) and the well-established methods which prove their validity for the analysis of this binary mixture. The methods were validated as per ICH guidelines regarding accuracy, precision, repeatability and robustness; which were found to be within the acceptable limits. The new methods were simple, sensitive and don't need a special program, so they could be easily applied as alternative methods to LC methods in quality control laboratories lacking the required facilities for those techniques.
author	LOTFY,HAYAM M HASSAN,NAGIBA Y ELGIZAWY,SAMIA M SALEH,SARAH S
author_facet	LOTFY,HAYAM M HASSAN,NAGIBA Y ELGIZAWY,SAMIA M SALEH,SARAH S
author_sort	LOTFY,HAYAM M
title	COMPARATIVE STUDY OF NEW SPECTROPHOTOMETRIC METHODS; AN APPLICATION ON PHARMACEUTICAL BINARY MIXTURE OF CIPROFLOXACIN HYDROCHLORIDE AND HYDROCORTISONE
title_short	COMPARATIVE STUDY OF NEW SPECTROPHOTOMETRIC METHODS; AN APPLICATION ON PHARMACEUTICAL BINARY MIXTURE OF CIPROFLOXACIN HYDROCHLORIDE AND HYDROCORTISONE
title_full	COMPARATIVE STUDY OF NEW SPECTROPHOTOMETRIC METHODS; AN APPLICATION ON PHARMACEUTICAL BINARY MIXTURE OF CIPROFLOXACIN HYDROCHLORIDE AND HYDROCORTISONE
title_fullStr	COMPARATIVE STUDY OF NEW SPECTROPHOTOMETRIC METHODS; AN APPLICATION ON PHARMACEUTICAL BINARY MIXTURE OF CIPROFLOXACIN HYDROCHLORIDE AND HYDROCORTISONE
title_full_unstemmed	COMPARATIVE STUDY OF NEW SPECTROPHOTOMETRIC METHODS; AN APPLICATION ON PHARMACEUTICAL BINARY MIXTURE OF CIPROFLOXACIN HYDROCHLORIDE AND HYDROCORTISONE
title_sort	comparative study of new spectrophotometric methods; an application on pharmaceutical binary mixture of ciprofloxacin hydrochloride and hydrocortisone
publisher	Sociedad Chilena de Química
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072013000300022
work_keys_str_mv	AT lotfyhayamm comparativestudyofnewspectrophotometricmethodsanapplicationonpharmaceuticalbinarymixtureofciprofloxacinhydrochlorideandhydrocortisone AT hassannagibay comparativestudyofnewspectrophotometricmethodsanapplicationonpharmaceuticalbinarymixtureofciprofloxacinhydrochlorideandhydrocortisone AT elgizawysamiam comparativestudyofnewspectrophotometricmethodsanapplicationonpharmaceuticalbinarymixtureofciprofloxacinhydrochlorideandhydrocortisone AT salehsarahs comparativestudyofnewspectrophotometricmethodsanapplicationonpharmaceuticalbinarymixtureofciprofloxacinhydrochlorideandhydrocortisone
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COMPARATIVE STUDY OF NEW SPECTROPHOTOMETRIC METHODS; AN APPLICATION ON PHARMACEUTICAL BINARY MIXTURE OF CIPROFLOXACIN HYDROCHLORIDE AND HYDROCORTISONE

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