ARSENIC SPECIES-BINDING PROTEINS IN HUMAN CARDIOVASCULAR AND MUSCLE TISSUES

The intracellular As-protein binding in cytosol and methanol-water extract of the auricle and saphene tissues of As impacted people was evaluated by bidimensional size exclusion FPLC-UV-ICP-MS. The fractionation of cytosol using Superdex, Phenomenex and MonoQ HR 5/5 columns, shows that As was distri...

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Autores principales:	PIZARRO,I, ROMAN-SILVA,D. A, GÓMEZ,M, CÁMARA,C, PALACIOS,M. A
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2013
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072013000400037
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spelling	oai:scielo:S0717-970720130004000372014-09-02ARSENIC SPECIES-BINDING PROTEINS IN HUMAN CARDIOVASCULAR AND MUSCLE TISSUESPIZARRO,IROMAN-SILVA,D. AGÓMEZ,MCÁMARA,CPALACIOS,M. AThe intracellular As-protein binding in cytosol and methanol-water extract of the auricle and saphene tissues of As impacted people was evaluated by bidimensional size exclusion FPLC-UV-ICP-MS. The fractionation of cytosol using Superdex, Phenomenex and MonoQ HR 5/5 columns, shows that As was distributed in a wide range of contiguous fractions of each column, being 8, 25, 50 % the percentages of As in the collected fractions, respectively. Arsenic a sulphur coelute when FPLC-UV-ICP-MS was applied, which could implicate that As is bound to bio-compounds of different molecular mass through vicinal sulphur groups. The monitoring of S, Cu and P. In the methanol: water extracts a similar study than performed with the cytosol using preparative gel chromatography on Sephadex G-75 and Shephadex G-100 columns. A very low As and protein contain were found in the different fractions of both SEC fractionating series. A similar As-protein association to that found in the cytosol after fractionating with MonoQ HR 5/5 was observed for auricle and saphene. Inorganic and methylated As speciation in the 20 - 26 cytosol fractions obtained within the Phenomenex column was performed by HPLC-ICP-MS using the Hamilton PRP-X100 column. Only As(III) and As(V) were present and the results obtained shows that the As(III)/As(V) ratio is constant in most cases. Direct evidence of the existence of As-binding peptides in auricle and saphene vein from arsenic impacted human beings has have been obtained which was previously reported by means of novo peptide synthesis.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.58 n.4 20132013-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072013000400037en10.4067/S0717-97072013000400037
institution	Scielo Chile
collection	Scielo Chile
language	English
description	The intracellular As-protein binding in cytosol and methanol-water extract of the auricle and saphene tissues of As impacted people was evaluated by bidimensional size exclusion FPLC-UV-ICP-MS. The fractionation of cytosol using Superdex, Phenomenex and MonoQ HR 5/5 columns, shows that As was distributed in a wide range of contiguous fractions of each column, being 8, 25, 50 % the percentages of As in the collected fractions, respectively. Arsenic a sulphur coelute when FPLC-UV-ICP-MS was applied, which could implicate that As is bound to bio-compounds of different molecular mass through vicinal sulphur groups. The monitoring of S, Cu and P. In the methanol: water extracts a similar study than performed with the cytosol using preparative gel chromatography on Sephadex G-75 and Shephadex G-100 columns. A very low As and protein contain were found in the different fractions of both SEC fractionating series. A similar As-protein association to that found in the cytosol after fractionating with MonoQ HR 5/5 was observed for auricle and saphene. Inorganic and methylated As speciation in the 20 - 26 cytosol fractions obtained within the Phenomenex column was performed by HPLC-ICP-MS using the Hamilton PRP-X100 column. Only As(III) and As(V) were present and the results obtained shows that the As(III)/As(V) ratio is constant in most cases. Direct evidence of the existence of As-binding peptides in auricle and saphene vein from arsenic impacted human beings has have been obtained which was previously reported by means of novo peptide synthesis.
author	PIZARRO,I ROMAN-SILVA,D. A GÓMEZ,M CÁMARA,C PALACIOS,M. A
spellingShingle	PIZARRO,I ROMAN-SILVA,D. A GÓMEZ,M CÁMARA,C PALACIOS,M. A ARSENIC SPECIES-BINDING PROTEINS IN HUMAN CARDIOVASCULAR AND MUSCLE TISSUES
author_facet	PIZARRO,I ROMAN-SILVA,D. A GÓMEZ,M CÁMARA,C PALACIOS,M. A
author_sort	PIZARRO,I
title	ARSENIC SPECIES-BINDING PROTEINS IN HUMAN CARDIOVASCULAR AND MUSCLE TISSUES
title_short	ARSENIC SPECIES-BINDING PROTEINS IN HUMAN CARDIOVASCULAR AND MUSCLE TISSUES
title_full	ARSENIC SPECIES-BINDING PROTEINS IN HUMAN CARDIOVASCULAR AND MUSCLE TISSUES
title_fullStr	ARSENIC SPECIES-BINDING PROTEINS IN HUMAN CARDIOVASCULAR AND MUSCLE TISSUES
title_full_unstemmed	ARSENIC SPECIES-BINDING PROTEINS IN HUMAN CARDIOVASCULAR AND MUSCLE TISSUES
title_sort	arsenic species-binding proteins in human cardiovascular and muscle tissues
publisher	Sociedad Chilena de Química
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072013000400037
work_keys_str_mv	AT pizarroi arsenicspeciesbindingproteinsinhumancardiovascularandmuscletissues AT romansilvada arsenicspeciesbindingproteinsinhumancardiovascularandmuscletissues AT gomezm arsenicspeciesbindingproteinsinhumancardiovascularandmuscletissues AT camarac arsenicspeciesbindingproteinsinhumancardiovascularandmuscletissues AT palaciosma arsenicspeciesbindingproteinsinhumancardiovascularandmuscletissues
_version_	1718445502806097920

ARSENIC SPECIES-BINDING PROTEINS IN HUMAN CARDIOVASCULAR AND MUSCLE TISSUES

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