EVALUATION OF THE TOTAL AMOUNT OF PHENOL GROUPS IN COMPLEX MIXTURES BY FOLIN'S AND ORAC-FLUORESCEIN METHODOLOGIES

The total number of phenolic HO groups in a mixture and their average reactivity are fundamental parameters in the evaluation of the capacity of the sample to remove potentially damaging free radicals. The total number of phenolic groups is generally determined by Folin's method and several com...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	CAMPOS,ANA MARIA, LÓPEZ-ALARCÓN,CAMILO, LISSI,EDUARDO
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2013
Materias:	Phenols Folin's method ORAC-Fluorescein antioxidants
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072013000400054
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-97072013000400054
record_format	dspace
spelling	oai:scielo:S0717-970720130004000542014-09-02EVALUATION OF THE TOTAL AMOUNT OF PHENOL GROUPS IN COMPLEX MIXTURES BY FOLIN'S AND ORAC-FLUORESCEIN METHODOLOGIESCAMPOS,ANA MARIALÓPEZ-ALARCÓN,CAMILOLISSI,EDUARDO Phenols Folin's method ORAC-Fluorescein antioxidants The total number of phenolic HO groups in a mixture and their average reactivity are fundamental parameters in the evaluation of the capacity of the sample to remove potentially damaging free radicals. The total number of phenolic groups is generally determined by Folin's method and several competitive experiments have been proposed to estimate the reactivity of the sample. Among those stand the Oxygen Radical Absorbance Capacity (ORAC) methodologies. However, when fluorescein is employed as target molecule (ORAC-FL methodology) the index is more related to the number of phenolic groups than to their average reactivity. In the present work it is shown that both indexes correlate with the number of phenolic groups in pure compounds and with the total number of reactive OH groups in complex mixtures (beverages, infusions, fruits and vegetables). This leads to a fair correlation between Folin's and ORAC-FL indexes in these samples.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.58 n.4 20132013-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072013000400054en10.4067/S0717-97072013000400054
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Phenols Folin's method ORAC-Fluorescein antioxidants
spellingShingle	Phenols Folin's method ORAC-Fluorescein antioxidants CAMPOS,ANA MARIA LÓPEZ-ALARCÓN,CAMILO LISSI,EDUARDO EVALUATION OF THE TOTAL AMOUNT OF PHENOL GROUPS IN COMPLEX MIXTURES BY FOLIN'S AND ORAC-FLUORESCEIN METHODOLOGIES
description	The total number of phenolic HO groups in a mixture and their average reactivity are fundamental parameters in the evaluation of the capacity of the sample to remove potentially damaging free radicals. The total number of phenolic groups is generally determined by Folin's method and several competitive experiments have been proposed to estimate the reactivity of the sample. Among those stand the Oxygen Radical Absorbance Capacity (ORAC) methodologies. However, when fluorescein is employed as target molecule (ORAC-FL methodology) the index is more related to the number of phenolic groups than to their average reactivity. In the present work it is shown that both indexes correlate with the number of phenolic groups in pure compounds and with the total number of reactive OH groups in complex mixtures (beverages, infusions, fruits and vegetables). This leads to a fair correlation between Folin's and ORAC-FL indexes in these samples.
author	CAMPOS,ANA MARIA LÓPEZ-ALARCÓN,CAMILO LISSI,EDUARDO
author_facet	CAMPOS,ANA MARIA LÓPEZ-ALARCÓN,CAMILO LISSI,EDUARDO
author_sort	CAMPOS,ANA MARIA
title	EVALUATION OF THE TOTAL AMOUNT OF PHENOL GROUPS IN COMPLEX MIXTURES BY FOLIN'S AND ORAC-FLUORESCEIN METHODOLOGIES
title_short	EVALUATION OF THE TOTAL AMOUNT OF PHENOL GROUPS IN COMPLEX MIXTURES BY FOLIN'S AND ORAC-FLUORESCEIN METHODOLOGIES
title_full	EVALUATION OF THE TOTAL AMOUNT OF PHENOL GROUPS IN COMPLEX MIXTURES BY FOLIN'S AND ORAC-FLUORESCEIN METHODOLOGIES
title_fullStr	EVALUATION OF THE TOTAL AMOUNT OF PHENOL GROUPS IN COMPLEX MIXTURES BY FOLIN'S AND ORAC-FLUORESCEIN METHODOLOGIES
title_full_unstemmed	EVALUATION OF THE TOTAL AMOUNT OF PHENOL GROUPS IN COMPLEX MIXTURES BY FOLIN'S AND ORAC-FLUORESCEIN METHODOLOGIES
title_sort	evaluation of the total amount of phenol groups in complex mixtures by folin's and orac-fluorescein methodologies
publisher	Sociedad Chilena de Química
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072013000400054
work_keys_str_mv	AT camposanamaria evaluationofthetotalamountofphenolgroupsincomplexmixturesbyfolinsandoracfluoresceinmethodologies AT lopezalarconcamilo evaluationofthetotalamountofphenolgroupsincomplexmixturesbyfolinsandoracfluoresceinmethodologies AT lissieduardo evaluationofthetotalamountofphenolgroupsincomplexmixturesbyfolinsandoracfluoresceinmethodologies
_version_	1718445505843822592

EVALUATION OF THE TOTAL AMOUNT OF PHENOL GROUPS IN COMPLEX MIXTURES BY FOLIN'S AND ORAC-FLUORESCEIN METHODOLOGIES

Ejemplares similares