LC DETERMINATION OF ROSUVASTATIN AND EZETIMIBE IN HUMAN PLASMA

A simple, precise, sensitive and validated reverse-phase high performance liquid chromatographic (RP-HPLC) method was developed for the simultaneous estimation of rosuvastatin and ezetimibe in human plasma. The method involved protein precipitation and extraction of both drugs from plasma using acet...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	ASHFAQ,MUHAMMAD, AHMAD,HAMAD, KHAN,ISLAM ULLAH, MUSTAFA,GHULAM
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2013
Materias:	HPLC Acetonitrile Diode Array Detector Rosuvastatin Ezetimibe
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072013000400059
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-97072013000400059
record_format	dspace
spelling	oai:scielo:S0717-970720130004000592014-09-02LC DETERMINATION OF ROSUVASTATIN AND EZETIMIBE IN HUMAN PLASMAASHFAQ,MUHAMMADAHMAD,HAMADKHAN,ISLAM ULLAHMUSTAFA,GHULAM HPLC Acetonitrile Diode Array Detector Rosuvastatin Ezetimibe A simple, precise, sensitive and validated reverse-phase high performance liquid chromatographic (RP-HPLC) method was developed for the simultaneous estimation of rosuvastatin and ezetimibe in human plasma. The method involved protein precipitation and extraction of both drugs from plasma using acetonitrile and then separation on a C-18 column. Both the analytes were detected at a wavelength of 240 nm using diode array detector. Mobile phase for the said separation consists of a mixture of 1.5% phosphoric acid and acetonitrile in 30:70, v/v ratio. Linearity was in the range of 0.32-267 mg/mL for rosuvastatin and 0.08-67 mg/mL for ezetimibe with coefficient of determination between 0.9997 and 0.9967. Limit of detection was 0.106 μg/mL for rosuvastatin and 0.026 mg/mL for ezetimibe whereas limit of quantification was 0.32 mg/mL and 0.08 mg/mL for rosuvastatin and ezetimibe respectively. Recovery of both the analytes was greater than 75% with RSD less than 15 %. The total run time was less than five min for the two components. The developed method can be fruitfully employed for the determination of these two drugs in human plasma.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.58 n.4 20132013-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072013000400059en10.4067/S0717-97072013000400059
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	HPLC Acetonitrile Diode Array Detector Rosuvastatin Ezetimibe
spellingShingle	HPLC Acetonitrile Diode Array Detector Rosuvastatin Ezetimibe ASHFAQ,MUHAMMAD AHMAD,HAMAD KHAN,ISLAM ULLAH MUSTAFA,GHULAM LC DETERMINATION OF ROSUVASTATIN AND EZETIMIBE IN HUMAN PLASMA
description	A simple, precise, sensitive and validated reverse-phase high performance liquid chromatographic (RP-HPLC) method was developed for the simultaneous estimation of rosuvastatin and ezetimibe in human plasma. The method involved protein precipitation and extraction of both drugs from plasma using acetonitrile and then separation on a C-18 column. Both the analytes were detected at a wavelength of 240 nm using diode array detector. Mobile phase for the said separation consists of a mixture of 1.5% phosphoric acid and acetonitrile in 30:70, v/v ratio. Linearity was in the range of 0.32-267 mg/mL for rosuvastatin and 0.08-67 mg/mL for ezetimibe with coefficient of determination between 0.9997 and 0.9967. Limit of detection was 0.106 μg/mL for rosuvastatin and 0.026 mg/mL for ezetimibe whereas limit of quantification was 0.32 mg/mL and 0.08 mg/mL for rosuvastatin and ezetimibe respectively. Recovery of both the analytes was greater than 75% with RSD less than 15 %. The total run time was less than five min for the two components. The developed method can be fruitfully employed for the determination of these two drugs in human plasma.
author	ASHFAQ,MUHAMMAD AHMAD,HAMAD KHAN,ISLAM ULLAH MUSTAFA,GHULAM
author_facet	ASHFAQ,MUHAMMAD AHMAD,HAMAD KHAN,ISLAM ULLAH MUSTAFA,GHULAM
author_sort	ASHFAQ,MUHAMMAD
title	LC DETERMINATION OF ROSUVASTATIN AND EZETIMIBE IN HUMAN PLASMA
title_short	LC DETERMINATION OF ROSUVASTATIN AND EZETIMIBE IN HUMAN PLASMA
title_full	LC DETERMINATION OF ROSUVASTATIN AND EZETIMIBE IN HUMAN PLASMA
title_fullStr	LC DETERMINATION OF ROSUVASTATIN AND EZETIMIBE IN HUMAN PLASMA
title_full_unstemmed	LC DETERMINATION OF ROSUVASTATIN AND EZETIMIBE IN HUMAN PLASMA
title_sort	lc determination of rosuvastatin and ezetimibe in human plasma
publisher	Sociedad Chilena de Química
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072013000400059
work_keys_str_mv	AT ashfaqmuhammad lcdeterminationofrosuvastatinandezetimibeinhumanplasma AT ahmadhamad lcdeterminationofrosuvastatinandezetimibeinhumanplasma AT khanislamullah lcdeterminationofrosuvastatinandezetimibeinhumanplasma AT mustafaghulam lcdeterminationofrosuvastatinandezetimibeinhumanplasma
_version_	1718445506755035136

LC DETERMINATION OF ROSUVASTATIN AND EZETIMIBE IN HUMAN PLASMA

Ejemplares similares