IODATE OXIDATION OF N-ACETYL L-CYSTEINE: APPLICATION IN DRUG DETERMINATION AND CHARACTERIZATION OF ITS OXIDATION AND DEGRADATION PRODUCT BY MASS SPECTROMETRY

A kinetic spectrophotometric method based on the initial rate measurement has been developed for the determination of N-acetyl L-cysteine. The developed method is based on the oxidation of N-acetyl L-cysteine with iodate. The reaction product was studied and characterized using the mass spectrometry...

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Autores principales:	SIDDIQUI,MASOOM RAZA, WABAIDUR,SAIKH MOHAMMAD, ALOTHMAN,ZIED A, RAHMAN,HABIBUR, ALAM,SARFARAZ, ALI,SAJID
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2014
Materias:	N- acetyl cysteine Initial rate method Spectrophotometry mass spectrometry
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072014000100013
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spelling	oai:scielo:S0717-970720140001000132015-11-12IODATE OXIDATION OF N-ACETYL L-CYSTEINE: APPLICATION IN DRUG DETERMINATION AND CHARACTERIZATION OF ITS OXIDATION AND DEGRADATION PRODUCT BY MASS SPECTROMETRYSIDDIQUI,MASOOM RAZAWABAIDUR,SAIKH MOHAMMADALOTHMAN,ZIED ARAHMAN,HABIBURALAM,SARFARAZALI,SAJID N- acetyl cysteine Initial rate method Spectrophotometry mass spectrometry A kinetic spectrophotometric method based on the initial rate measurement has been developed for the determination of N-acetyl L-cysteine. The developed method is based on the oxidation of N-acetyl L-cysteine with iodate. The reaction product was studied and characterized using the mass spectrometry and the structure of the product was proposed. From the mass spectrometric studies it was concluded that the oxidation of the drug resulted in the formation of a disulfide. The developed method was validated as per the guidelines of international conference on harmonization. The developed initial rate method was found to be linear in the concentration range of 1.25 - 30 µg ml-1. The detection and quantitation limits were found to be 0.018 and 0.056 µg ml-1. In the current study, the degradation product of N-acetyl L cysteine was also prepared and identified using mass spectrometry.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.59 n.1 20142014-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072014000100013en10.4067/S0717-97072014000100013
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	N- acetyl cysteine Initial rate method Spectrophotometry mass spectrometry
spellingShingle	N- acetyl cysteine Initial rate method Spectrophotometry mass spectrometry SIDDIQUI,MASOOM RAZA WABAIDUR,SAIKH MOHAMMAD ALOTHMAN,ZIED A RAHMAN,HABIBUR ALAM,SARFARAZ ALI,SAJID IODATE OXIDATION OF N-ACETYL L-CYSTEINE: APPLICATION IN DRUG DETERMINATION AND CHARACTERIZATION OF ITS OXIDATION AND DEGRADATION PRODUCT BY MASS SPECTROMETRY
description	A kinetic spectrophotometric method based on the initial rate measurement has been developed for the determination of N-acetyl L-cysteine. The developed method is based on the oxidation of N-acetyl L-cysteine with iodate. The reaction product was studied and characterized using the mass spectrometry and the structure of the product was proposed. From the mass spectrometric studies it was concluded that the oxidation of the drug resulted in the formation of a disulfide. The developed method was validated as per the guidelines of international conference on harmonization. The developed initial rate method was found to be linear in the concentration range of 1.25 - 30 µg ml-1. The detection and quantitation limits were found to be 0.018 and 0.056 µg ml-1. In the current study, the degradation product of N-acetyl L cysteine was also prepared and identified using mass spectrometry.
author	SIDDIQUI,MASOOM RAZA WABAIDUR,SAIKH MOHAMMAD ALOTHMAN,ZIED A RAHMAN,HABIBUR ALAM,SARFARAZ ALI,SAJID
author_facet	SIDDIQUI,MASOOM RAZA WABAIDUR,SAIKH MOHAMMAD ALOTHMAN,ZIED A RAHMAN,HABIBUR ALAM,SARFARAZ ALI,SAJID
author_sort	SIDDIQUI,MASOOM RAZA
title	IODATE OXIDATION OF N-ACETYL L-CYSTEINE: APPLICATION IN DRUG DETERMINATION AND CHARACTERIZATION OF ITS OXIDATION AND DEGRADATION PRODUCT BY MASS SPECTROMETRY
title_short	IODATE OXIDATION OF N-ACETYL L-CYSTEINE: APPLICATION IN DRUG DETERMINATION AND CHARACTERIZATION OF ITS OXIDATION AND DEGRADATION PRODUCT BY MASS SPECTROMETRY
title_full	IODATE OXIDATION OF N-ACETYL L-CYSTEINE: APPLICATION IN DRUG DETERMINATION AND CHARACTERIZATION OF ITS OXIDATION AND DEGRADATION PRODUCT BY MASS SPECTROMETRY
title_fullStr	IODATE OXIDATION OF N-ACETYL L-CYSTEINE: APPLICATION IN DRUG DETERMINATION AND CHARACTERIZATION OF ITS OXIDATION AND DEGRADATION PRODUCT BY MASS SPECTROMETRY
title_full_unstemmed	IODATE OXIDATION OF N-ACETYL L-CYSTEINE: APPLICATION IN DRUG DETERMINATION AND CHARACTERIZATION OF ITS OXIDATION AND DEGRADATION PRODUCT BY MASS SPECTROMETRY
title_sort	iodate oxidation of n-acetyl l-cysteine: application in drug determination and characterization of its oxidation and degradation product by mass spectrometry
publisher	Sociedad Chilena de Química
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072014000100013
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IODATE OXIDATION OF N-ACETYL L-CYSTEINE: APPLICATION IN DRUG DETERMINATION AND CHARACTERIZATION OF ITS OXIDATION AND DEGRADATION PRODUCT BY MASS SPECTROMETRY

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