DEVELOPMENT OF VALIDATED HPTLC METHOD FOR QUANTIFICATION OF TRAZODONE IN HUMAN SERUM

A high performance thin-layer chromatographic (HPTLC) method for quantitative analysis of trazodone in human serum has been developed and validated. Trazodone was extracted with n-hexane: isoamyl alcohol (99:1). Chromatographic separation was performed on silica gel 60 F 254 HPTLC plates, with tolue...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	MENNICKENT,SIGRID, GONZÁLEZ,ANA, VEGA,MARIO, RÍOS,GISELA, DIEGO,MARTA DE
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2014
Materias:	Trazodone HPTLC quantitative analysis serum drugs
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072014000200002
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-97072014000200002
record_format	dspace
spelling	oai:scielo:S0717-970720140002000022014-11-05DEVELOPMENT OF VALIDATED HPTLC METHOD FOR QUANTIFICATION OF TRAZODONE IN HUMAN SERUMMENNICKENT,SIGRIDGONZÁLEZ,ANAVEGA,MARIORÍOS,GISELADIEGO,MARTA DE Trazodone HPTLC quantitative analysis serum drugs A high performance thin-layer chromatographic (HPTLC) method for quantitative analysis of trazodone in human serum has been developed and validated. Trazodone was extracted with n-hexane: isoamyl alcohol (99:1). Chromatographic separation was performed on silica gel 60 F 254 HPTLC plates, with toluene: acetone: ethanol: ammonium (9:7:2:0.5, v/v/v/v) as mobile phase. Densitometric evaluation was carried out at 290 nm. There was no chromatographic interference between trazodone and its major metabolite, m-chlorophenilpiperazine (Rf 0.82 and 0.39, respectively). Regression analysis of the calibration plot revealed good linearity (r=0.999) over the range of 20.00 and 200.00 ng/band, corresponding to 0.20 and 2.00 ng/µL of trazodone in human serum after extraction process and applying 10 µL to the chromatographic plates. The % RSD of intra-assay and inter-assay precision, were in the range of 0.98% to 2.97% (n=3) and 1.06% to 3.54% (n=9), respectively. LOD and LOQ were found to be 0.016ng/µL, and 0.048ng/µL, and the recovery values, of trazodone from serum, were between 92.52 % and 96.73 %. This method was successfully applied to quantify trazodone in patient serum samples. In conclusion, the developed method might be useful for the quantitative determination of trazodone in human serum, as a tool to evaluate patient adherence to their pharmacotherapy with this drug.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.59 n.2 20142014-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072014000200002en10.4067/S0717-97072014000200002
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Trazodone HPTLC quantitative analysis serum drugs
spellingShingle	Trazodone HPTLC quantitative analysis serum drugs MENNICKENT,SIGRID GONZÁLEZ,ANA VEGA,MARIO RÍOS,GISELA DIEGO,MARTA DE DEVELOPMENT OF VALIDATED HPTLC METHOD FOR QUANTIFICATION OF TRAZODONE IN HUMAN SERUM
description	A high performance thin-layer chromatographic (HPTLC) method for quantitative analysis of trazodone in human serum has been developed and validated. Trazodone was extracted with n-hexane: isoamyl alcohol (99:1). Chromatographic separation was performed on silica gel 60 F 254 HPTLC plates, with toluene: acetone: ethanol: ammonium (9:7:2:0.5, v/v/v/v) as mobile phase. Densitometric evaluation was carried out at 290 nm. There was no chromatographic interference between trazodone and its major metabolite, m-chlorophenilpiperazine (Rf 0.82 and 0.39, respectively). Regression analysis of the calibration plot revealed good linearity (r=0.999) over the range of 20.00 and 200.00 ng/band, corresponding to 0.20 and 2.00 ng/µL of trazodone in human serum after extraction process and applying 10 µL to the chromatographic plates. The % RSD of intra-assay and inter-assay precision, were in the range of 0.98% to 2.97% (n=3) and 1.06% to 3.54% (n=9), respectively. LOD and LOQ were found to be 0.016ng/µL, and 0.048ng/µL, and the recovery values, of trazodone from serum, were between 92.52 % and 96.73 %. This method was successfully applied to quantify trazodone in patient serum samples. In conclusion, the developed method might be useful for the quantitative determination of trazodone in human serum, as a tool to evaluate patient adherence to their pharmacotherapy with this drug.
author	MENNICKENT,SIGRID GONZÁLEZ,ANA VEGA,MARIO RÍOS,GISELA DIEGO,MARTA DE
author_facet	MENNICKENT,SIGRID GONZÁLEZ,ANA VEGA,MARIO RÍOS,GISELA DIEGO,MARTA DE
author_sort	MENNICKENT,SIGRID
title	DEVELOPMENT OF VALIDATED HPTLC METHOD FOR QUANTIFICATION OF TRAZODONE IN HUMAN SERUM
title_short	DEVELOPMENT OF VALIDATED HPTLC METHOD FOR QUANTIFICATION OF TRAZODONE IN HUMAN SERUM
title_full	DEVELOPMENT OF VALIDATED HPTLC METHOD FOR QUANTIFICATION OF TRAZODONE IN HUMAN SERUM
title_fullStr	DEVELOPMENT OF VALIDATED HPTLC METHOD FOR QUANTIFICATION OF TRAZODONE IN HUMAN SERUM
title_full_unstemmed	DEVELOPMENT OF VALIDATED HPTLC METHOD FOR QUANTIFICATION OF TRAZODONE IN HUMAN SERUM
title_sort	development of validated hptlc method for quantification of trazodone in human serum
publisher	Sociedad Chilena de Química
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072014000200002
work_keys_str_mv	AT mennickentsigrid developmentofvalidatedhptlcmethodforquantificationoftrazodoneinhumanserum AT gonzalezana developmentofvalidatedhptlcmethodforquantificationoftrazodoneinhumanserum AT vegamario developmentofvalidatedhptlcmethodforquantificationoftrazodoneinhumanserum AT riosgisela developmentofvalidatedhptlcmethodforquantificationoftrazodoneinhumanserum AT diegomartade developmentofvalidatedhptlcmethodforquantificationoftrazodoneinhumanserum
_version_	1718445515470798848

DEVELOPMENT OF VALIDATED HPTLC METHOD FOR QUANTIFICATION OF TRAZODONE IN HUMAN SERUM

Ejemplares similares