SENSING IMMOBILIZED MOLECULES OF STREPTAVIDIN ON A SILICON SURFACE BY MALDI-TOF MASS SPECTROMETRY AND FLUORESCENCE MICROSCOPY

A hydrogen-terminated Si (111) surface was modified to form an aminoterminated monolayer for immobilization of streptavidin. Cleavage of an N-(ω-undecylenyl)-phthalimide covered surface using hidrazine yields an amino group-modified surface, which serves as a substrate for the attachment of...

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Autores principales:	EYLEEN,ARAYA, MARCELO J,KOGAN, ALEIX G,GUELL, CARLOS A,ESCOBAR, FAUSTO,SANZ
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2014
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072014000200013
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spelling	oai:scielo:S0717-970720140002000132014-11-05SENSING IMMOBILIZED MOLECULES OF STREPTAVIDIN ON A SILICON SURFACE BY MALDI-TOF MASS SPECTROMETRY AND FLUORESCENCE MICROSCOPYEYLEEN,ARAYAMARCELO J,KOGANALEIX G,GUELLCARLOS A,ESCOBARFAUSTO,SANZA hydrogen-terminated Si (111) surface was modified to form an aminoterminated monolayer for immobilization of streptavidin. Cleavage of an N-(ω-undecylenyl)-phthalimide covered surface using hidrazine yields an amino group-modified surface, which serves as a substrate for the attachment of biotin and subsequently streptavidin. We used surface analytical techniques to characterize the surface and to control the course of functionalization before the immobilization of streptavidin. To confirm the presence of the streptavidin Texas red on the surface two powerful techniques available in a standard biochemical laboratory are used, Fluorescence Microscopy and MALDI-TOF that allow us to detect and determine the immobilized streptavidin. This work provides an avenue for the development of devices in which the exquisite binding specificity of biomolecular recognition is directly coupled to a biosensor. In addition, we have demonstrated that MALDI-TOF and fluorescence microscopy are useful techniques for the characterization of silicon functionalized surfaces.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.59 n.2 20142014-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072014000200013en10.4067/S0717-97072014000200013
institution	Scielo Chile
collection	Scielo Chile
language	English
description	A hydrogen-terminated Si (111) surface was modified to form an aminoterminated monolayer for immobilization of streptavidin. Cleavage of an N-(ω-undecylenyl)-phthalimide covered surface using hidrazine yields an amino group-modified surface, which serves as a substrate for the attachment of biotin and subsequently streptavidin. We used surface analytical techniques to characterize the surface and to control the course of functionalization before the immobilization of streptavidin. To confirm the presence of the streptavidin Texas red on the surface two powerful techniques available in a standard biochemical laboratory are used, Fluorescence Microscopy and MALDI-TOF that allow us to detect and determine the immobilized streptavidin. This work provides an avenue for the development of devices in which the exquisite binding specificity of biomolecular recognition is directly coupled to a biosensor. In addition, we have demonstrated that MALDI-TOF and fluorescence microscopy are useful techniques for the characterization of silicon functionalized surfaces.
author	EYLEEN,ARAYA MARCELO J,KOGAN ALEIX G,GUELL CARLOS A,ESCOBAR FAUSTO,SANZ
spellingShingle	EYLEEN,ARAYA MARCELO J,KOGAN ALEIX G,GUELL CARLOS A,ESCOBAR FAUSTO,SANZ SENSING IMMOBILIZED MOLECULES OF STREPTAVIDIN ON A SILICON SURFACE BY MALDI-TOF MASS SPECTROMETRY AND FLUORESCENCE MICROSCOPY
author_facet	EYLEEN,ARAYA MARCELO J,KOGAN ALEIX G,GUELL CARLOS A,ESCOBAR FAUSTO,SANZ
author_sort	EYLEEN,ARAYA
title	SENSING IMMOBILIZED MOLECULES OF STREPTAVIDIN ON A SILICON SURFACE BY MALDI-TOF MASS SPECTROMETRY AND FLUORESCENCE MICROSCOPY
title_short	SENSING IMMOBILIZED MOLECULES OF STREPTAVIDIN ON A SILICON SURFACE BY MALDI-TOF MASS SPECTROMETRY AND FLUORESCENCE MICROSCOPY
title_full	SENSING IMMOBILIZED MOLECULES OF STREPTAVIDIN ON A SILICON SURFACE BY MALDI-TOF MASS SPECTROMETRY AND FLUORESCENCE MICROSCOPY
title_fullStr	SENSING IMMOBILIZED MOLECULES OF STREPTAVIDIN ON A SILICON SURFACE BY MALDI-TOF MASS SPECTROMETRY AND FLUORESCENCE MICROSCOPY
title_full_unstemmed	SENSING IMMOBILIZED MOLECULES OF STREPTAVIDIN ON A SILICON SURFACE BY MALDI-TOF MASS SPECTROMETRY AND FLUORESCENCE MICROSCOPY
title_sort	sensing immobilized molecules of streptavidin on a silicon surface by maldi-tof mass spectrometry and fluorescence microscopy
publisher	Sociedad Chilena de Química
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072014000200013
work_keys_str_mv	AT eyleenaraya sensingimmobilizedmoleculesofstreptavidinonasiliconsurfacebymalditofmassspectrometryandfluorescencemicroscopy AT marcelojkogan sensingimmobilizedmoleculesofstreptavidinonasiliconsurfacebymalditofmassspectrometryandfluorescencemicroscopy AT aleixgguell sensingimmobilizedmoleculesofstreptavidinonasiliconsurfacebymalditofmassspectrometryandfluorescencemicroscopy AT carlosaescobar sensingimmobilizedmoleculesofstreptavidinonasiliconsurfacebymalditofmassspectrometryandfluorescencemicroscopy AT faustosanz sensingimmobilizedmoleculesofstreptavidinonasiliconsurfacebymalditofmassspectrometryandfluorescencemicroscopy
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SENSING IMMOBILIZED MOLECULES OF STREPTAVIDIN ON A SILICON SURFACE BY MALDI-TOF MASS SPECTROMETRY AND FLUORESCENCE MICROSCOPY

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