EFFECT OF LOW CONCENTRATION OF DHPC ON THE DPPC/SM BILAYER

EFFECT OF LOW CONCENTRATION OF DHPC ON THE DPPC/SM BILAYER

The organization of bilayers depends on the physicochemical and structural properties of the molecules that compose the membrane. The phospholipid molecular shape depends on the relative volumes of their polar headgroups and non-polar acyl chains. In this work, we study the effect of DHPC concentrat...

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Autores principales: MONTECINOS,RODRIGO, NEIRA-JIMENEZ,STEPHANIE
Lenguaje:English
Publicado: Sociedad Chilena de Química 2014
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072014000200025
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spelling oai:scielo:S0717-970720140002000252014-11-05EFFECT OF LOW CONCENTRATION OF DHPC ON THE DPPC/SM BILAYERMONTECINOS,RODRIGONEIRA-JIMENEZ,STEPHANIEThe organization of bilayers depends on the physicochemical and structural properties of the molecules that compose the membrane. The phospholipid molecular shape depends on the relative volumes of their polar headgroups and non-polar acyl chains. In this work, we study the effect of DHPC concentration over the bilayer of large unilamellar vesicles (LUV) prepared with different proportions of dipalmitoylphosphatidylcholine / sphingomyelin / dihexanoylphosphatidylcholine (DPPC/SM/DHPC) using fluorescence spectroscopy and molecular dynamics (MD) simulations. In this work, we use Laurdan's generalized polarization (GP) and 1,6-diphenyl-1,3,5-hexatriene (DPH) fluorescence anisotropy to observe the effect of DHPC concentration on dynamic properties of both, the interface and hydrophobic region. Detailed information, at atomistic scale, was obtained by trajectory calculations of accessible solvent area (ASA), partial densities and order parameters using Molecular Dynamics (MD). The results indicate that DHPC induces global packing defects in the membrane, favoring the liquid crystalline state for the system with higher concentration of DHPC.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.59 n.2 20142014-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072014000200025en10.4067/S0717-97072014000200025
institution Scielo Chile
collection Scielo Chile
language English
description The organization of bilayers depends on the physicochemical and structural properties of the molecules that compose the membrane. The phospholipid molecular shape depends on the relative volumes of their polar headgroups and non-polar acyl chains. In this work, we study the effect of DHPC concentration over the bilayer of large unilamellar vesicles (LUV) prepared with different proportions of dipalmitoylphosphatidylcholine / sphingomyelin / dihexanoylphosphatidylcholine (DPPC/SM/DHPC) using fluorescence spectroscopy and molecular dynamics (MD) simulations. In this work, we use Laurdan's generalized polarization (GP) and 1,6-diphenyl-1,3,5-hexatriene (DPH) fluorescence anisotropy to observe the effect of DHPC concentration on dynamic properties of both, the interface and hydrophobic region. Detailed information, at atomistic scale, was obtained by trajectory calculations of accessible solvent area (ASA), partial densities and order parameters using Molecular Dynamics (MD). The results indicate that DHPC induces global packing defects in the membrane, favoring the liquid crystalline state for the system with higher concentration of DHPC.
author MONTECINOS,RODRIGO
NEIRA-JIMENEZ,STEPHANIE
spellingShingle MONTECINOS,RODRIGO
NEIRA-JIMENEZ,STEPHANIE
EFFECT OF LOW CONCENTRATION OF DHPC ON THE DPPC/SM BILAYER
author_facet MONTECINOS,RODRIGO
NEIRA-JIMENEZ,STEPHANIE
author_sort MONTECINOS,RODRIGO
title EFFECT OF LOW CONCENTRATION OF DHPC ON THE DPPC/SM BILAYER
title_short EFFECT OF LOW CONCENTRATION OF DHPC ON THE DPPC/SM BILAYER
title_full EFFECT OF LOW CONCENTRATION OF DHPC ON THE DPPC/SM BILAYER
title_fullStr EFFECT OF LOW CONCENTRATION OF DHPC ON THE DPPC/SM BILAYER
title_full_unstemmed EFFECT OF LOW CONCENTRATION OF DHPC ON THE DPPC/SM BILAYER
title_sort effect of low concentration of dhpc on the dppc/sm bilayer
publisher Sociedad Chilena de Química
publishDate 2014
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072014000200025
work_keys_str_mv AT montecinosrodrigo effectoflowconcentrationofdhpconthedppcsmbilayer
AT neirajimenezstephanie effectoflowconcentrationofdhpconthedppcsmbilayer
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