Cr+6 REMOVAL BY INDIGENOUS BACTERIA IN CONJUNCTION WITH DIFFERENT BIOWASTE MATERIALS: AN ECOFRIENDLY APPROACH
Two Cr+6 reducing bacterial strains previously isolated from tannery effluents were used in the present study and identified as Pseudomonas aeruginosa Rb-I and Ochrobacterum intermedium (Rb-2) by 16S rRNA sequencing. Different biowaste materials (waste tea leaves, carrot juice pulp, dry leaves of eu...
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Lenguaje: | English |
Publicado: |
Sociedad Chilena de Química
2014
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Materias: | |
Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072014000300012 |
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Sumario: | Two Cr+6 reducing bacterial strains previously isolated from tannery effluents were used in the present study and identified as Pseudomonas aeruginosa Rb-I and Ochrobacterum intermedium (Rb-2) by 16S rRNA sequencing. Different biowaste materials (waste tea leaves, carrot juice pulp, dry leaves of eucalyptus and rice husk) were assessed for sorption / removal of Cr+6 from aqueous solution of K2Cr2O4. Feasibility of mono and mixed cultures of indigenous bacterial strains was evaluated for Cr+6 removal in conjunction with different biowaste materials. Among all tested biowastes materials, waste tea leaves showed optimum removal of Cr+6 from metal solution alone (77.1%) as well as in combination with bacterial strains (99.4%) after 720 minutes of contact time. Mixed culture of bacterial strains was found to be more efficient in Cr+6 removal than monoculture. The contact time of 720 minutes, pH 7, biomass concentration of 2.5 gram 100 mL-1 , 37°C and shaking speed of 100 rpm were found to be most optimum for optimum Cr+6 sorption alone as well as in combination with bacterial strains. Fourier transform infrared spectroscopy revealed that carboxyl, amino and OH groups present on the waste tea leaves played a significant role in the binding of Cr+6 ions with the biomass. The present study is unique in this respect that this approach involve both living and non-living materials and we could not find any report documenting such findings up to our knowledge. |
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