DEVELOPMENT AND VALIDATION OF A REVERSED-PHASE HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF DICYCLOMINE HYDROCHLORIDE AND FAMOTIDINE IN BULK AND TABLETS

In the present study an isocratic reverse phase-high performance liquid chromatography (RP-HPLC) method was developed and validated for simultaneous estimation of dicyclomine hydrochloride (DCY) and famotidine (FAM) in bulk and pharmaceutical dosage forms. The separation was achieved on a Phenomex G...

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Autores principales:	DONDA,SHAILESH T, BAVISKAR,VISHAL B, DESHMUKH,PRASHANT K, BARI,SANJAY B, PATIL,PRAVIN O
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2014
Materias:	Dicyclomine hydrochloride Famotidine RP-HPLC
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072014000400007
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spelling	oai:scielo:S0717-970720140004000072015-01-09DEVELOPMENT AND VALIDATION OF A REVERSED-PHASE HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF DICYCLOMINE HYDROCHLORIDE AND FAMOTIDINE IN BULK AND TABLETSDONDA,SHAILESH TBAVISKAR,VISHAL BDESHMUKH,PRASHANT KBARI,SANJAY BPATIL,PRAVIN O Dicyclomine hydrochloride Famotidine RP-HPLC In the present study an isocratic reverse phase-high performance liquid chromatography (RP-HPLC) method was developed and validated for simultaneous estimation of dicyclomine hydrochloride (DCY) and famotidine (FAM) in bulk and pharmaceutical dosage forms. The separation was achieved on a Phenomex Gemini C column (4.6 x 250 mm, 5μ particle size) using a mixture of methanol and 0.1% triethylamine (TEA) pH 3.0 (adjusted with orthophosphoric acid) in the ratio of 40:60 (v/v) as a mobile phase. The flow rate was 1.0 mL min-1, column temperature was kept at 28 0 C and detection was monitored at 270 nm. The retention time of dicyclomine hydrochloride and famotidine was found to be 10.72 and 2.72 min respectively. Recovery studies were satisfactory and the correlation coefficient for FAM and DCY were 0.9947 and 0.9935 respectively, indicates linearity of the methods within the limits. The developed method can be used for routine quality control simultaneous analysis of DCY and FAM in tablet dosage form without any interference of excipients.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.59 n.4 20142014-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072014000400007en10.4067/S0717-97072014000400007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Dicyclomine hydrochloride Famotidine RP-HPLC
spellingShingle	Dicyclomine hydrochloride Famotidine RP-HPLC DONDA,SHAILESH T BAVISKAR,VISHAL B DESHMUKH,PRASHANT K BARI,SANJAY B PATIL,PRAVIN O DEVELOPMENT AND VALIDATION OF A REVERSED-PHASE HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF DICYCLOMINE HYDROCHLORIDE AND FAMOTIDINE IN BULK AND TABLETS
description	In the present study an isocratic reverse phase-high performance liquid chromatography (RP-HPLC) method was developed and validated for simultaneous estimation of dicyclomine hydrochloride (DCY) and famotidine (FAM) in bulk and pharmaceutical dosage forms. The separation was achieved on a Phenomex Gemini C column (4.6 x 250 mm, 5μ particle size) using a mixture of methanol and 0.1% triethylamine (TEA) pH 3.0 (adjusted with orthophosphoric acid) in the ratio of 40:60 (v/v) as a mobile phase. The flow rate was 1.0 mL min-1, column temperature was kept at 28 0 C and detection was monitored at 270 nm. The retention time of dicyclomine hydrochloride and famotidine was found to be 10.72 and 2.72 min respectively. Recovery studies were satisfactory and the correlation coefficient for FAM and DCY were 0.9947 and 0.9935 respectively, indicates linearity of the methods within the limits. The developed method can be used for routine quality control simultaneous analysis of DCY and FAM in tablet dosage form without any interference of excipients.
author	DONDA,SHAILESH T BAVISKAR,VISHAL B DESHMUKH,PRASHANT K BARI,SANJAY B PATIL,PRAVIN O
author_facet	DONDA,SHAILESH T BAVISKAR,VISHAL B DESHMUKH,PRASHANT K BARI,SANJAY B PATIL,PRAVIN O
author_sort	DONDA,SHAILESH T
title	DEVELOPMENT AND VALIDATION OF A REVERSED-PHASE HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF DICYCLOMINE HYDROCHLORIDE AND FAMOTIDINE IN BULK AND TABLETS
title_short	DEVELOPMENT AND VALIDATION OF A REVERSED-PHASE HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF DICYCLOMINE HYDROCHLORIDE AND FAMOTIDINE IN BULK AND TABLETS
title_full	DEVELOPMENT AND VALIDATION OF A REVERSED-PHASE HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF DICYCLOMINE HYDROCHLORIDE AND FAMOTIDINE IN BULK AND TABLETS
title_fullStr	DEVELOPMENT AND VALIDATION OF A REVERSED-PHASE HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF DICYCLOMINE HYDROCHLORIDE AND FAMOTIDINE IN BULK AND TABLETS
title_full_unstemmed	DEVELOPMENT AND VALIDATION OF A REVERSED-PHASE HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF DICYCLOMINE HYDROCHLORIDE AND FAMOTIDINE IN BULK AND TABLETS
title_sort	development and validation of a reversed-phase hplc method for the simultaneous estimation of dicyclomine hydrochloride and famotidine in bulk and tablets
publisher	Sociedad Chilena de Química
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072014000400007
work_keys_str_mv	AT dondashailesht developmentandvalidationofareversedphasehplcmethodforthesimultaneousestimationofdicyclominehydrochlorideandfamotidineinbulkandtablets AT baviskarvishalb developmentandvalidationofareversedphasehplcmethodforthesimultaneousestimationofdicyclominehydrochlorideandfamotidineinbulkandtablets AT deshmukhprashantk developmentandvalidationofareversedphasehplcmethodforthesimultaneousestimationofdicyclominehydrochlorideandfamotidineinbulkandtablets AT barisanjayb developmentandvalidationofareversedphasehplcmethodforthesimultaneousestimationofdicyclominehydrochlorideandfamotidineinbulkandtablets AT patilpravino developmentandvalidationofareversedphasehplcmethodforthesimultaneousestimationofdicyclominehydrochlorideandfamotidineinbulkandtablets
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DEVELOPMENT AND VALIDATION OF A REVERSED-PHASE HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF DICYCLOMINE HYDROCHLORIDE AND FAMOTIDINE IN BULK AND TABLETS

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