DEVELOPMENT OF AN EFFICIENT HPLC METHOD WITH PRE-COLUMN DERIVATIZATION FOR DETERMINATION OF ENANTIOMERIC PURITY OF 2-(AMINOMETHYL)-1-ETHYLPYRROLIDINE

The development of a simple and efficient analytical method for determination of enantiomeric purity of 2-(aminomethyl)-1-ethylpyrrolidine was presented. The method was based on pre-column derivatization of 2-(aminomethyl)-1-ethylpyrrolidine with 4-nitrobenzoic acid completing separation of the enan...

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Autores principales:	WANG,DAO-CAI, YAO,TIAN, QIAN,GUO-FEI, PU,LING-XIANG, YAO,SHUN, SONG,HANG
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2015
Materias:	HPLC enantiomeric separation 2-(aminomethyl)-1-ethylpyrrolidine pre-column derivatization
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072015000100002
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spelling	oai:scielo:S0717-970720150001000022015-04-21DEVELOPMENT OF AN EFFICIENT HPLC METHOD WITH PRE-COLUMN DERIVATIZATION FOR DETERMINATION OF ENANTIOMERIC PURITY OF 2-(AMINOMETHYL)-1-ETHYLPYRROLIDINEWANG,DAO-CAIYAO,TIANQIAN,GUO-FEIPU,LING-XIANGYAO,SHUNSONG,HANG HPLC enantiomeric separation 2-(aminomethyl)-1-ethylpyrrolidine pre-column derivatization The development of a simple and efficient analytical method for determination of enantiomeric purity of 2-(aminomethyl)-1-ethylpyrrolidine was presented. The method was based on pre-column derivatization of 2-(aminomethyl)-1-ethylpyrrolidine with 4-nitrobenzoic acid completing separation of the enantiomers on high performance liquid chromatographic (HPLC) chiral stationary phase (CSP). After optimizing all the effective parameters, the best performances were achieved on a Chiralcel OD-H analytical column (250 × 4.6 mm) using n-hexane: ethanol (98:2, v/v) containing 0.2% triethylamine as mobile phase with a flow rate of 1.0 mL min-1 at 25°C. Online UV and optical rotation (OR) detection was performed at 254 nm. The parameters which have an effect on chiral separation were investigated, including mobile phase additive, organic modifier type and concentration, column temperature and flow rate. The method was tested for precision, accuracy, linearity, limit of detection (LOD), limit of quantification (LOQ) and robustness. The present method is expected to be accurate, stable, rapid and sensitive and can be used for the determination of the enantiomeric purity of 2-(aminomethyl)-1-ethylpyrrolidine in bulk samples.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.60 n.1 20152015-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072015000100002en10.4067/S0717-97072015000100002
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	HPLC enantiomeric separation 2-(aminomethyl)-1-ethylpyrrolidine pre-column derivatization
spellingShingle	HPLC enantiomeric separation 2-(aminomethyl)-1-ethylpyrrolidine pre-column derivatization WANG,DAO-CAI YAO,TIAN QIAN,GUO-FEI PU,LING-XIANG YAO,SHUN SONG,HANG DEVELOPMENT OF AN EFFICIENT HPLC METHOD WITH PRE-COLUMN DERIVATIZATION FOR DETERMINATION OF ENANTIOMERIC PURITY OF 2-(AMINOMETHYL)-1-ETHYLPYRROLIDINE
description	The development of a simple and efficient analytical method for determination of enantiomeric purity of 2-(aminomethyl)-1-ethylpyrrolidine was presented. The method was based on pre-column derivatization of 2-(aminomethyl)-1-ethylpyrrolidine with 4-nitrobenzoic acid completing separation of the enantiomers on high performance liquid chromatographic (HPLC) chiral stationary phase (CSP). After optimizing all the effective parameters, the best performances were achieved on a Chiralcel OD-H analytical column (250 × 4.6 mm) using n-hexane: ethanol (98:2, v/v) containing 0.2% triethylamine as mobile phase with a flow rate of 1.0 mL min-1 at 25°C. Online UV and optical rotation (OR) detection was performed at 254 nm. The parameters which have an effect on chiral separation were investigated, including mobile phase additive, organic modifier type and concentration, column temperature and flow rate. The method was tested for precision, accuracy, linearity, limit of detection (LOD), limit of quantification (LOQ) and robustness. The present method is expected to be accurate, stable, rapid and sensitive and can be used for the determination of the enantiomeric purity of 2-(aminomethyl)-1-ethylpyrrolidine in bulk samples.
author	WANG,DAO-CAI YAO,TIAN QIAN,GUO-FEI PU,LING-XIANG YAO,SHUN SONG,HANG
author_facet	WANG,DAO-CAI YAO,TIAN QIAN,GUO-FEI PU,LING-XIANG YAO,SHUN SONG,HANG
author_sort	WANG,DAO-CAI
title	DEVELOPMENT OF AN EFFICIENT HPLC METHOD WITH PRE-COLUMN DERIVATIZATION FOR DETERMINATION OF ENANTIOMERIC PURITY OF 2-(AMINOMETHYL)-1-ETHYLPYRROLIDINE
title_short	DEVELOPMENT OF AN EFFICIENT HPLC METHOD WITH PRE-COLUMN DERIVATIZATION FOR DETERMINATION OF ENANTIOMERIC PURITY OF 2-(AMINOMETHYL)-1-ETHYLPYRROLIDINE
title_full	DEVELOPMENT OF AN EFFICIENT HPLC METHOD WITH PRE-COLUMN DERIVATIZATION FOR DETERMINATION OF ENANTIOMERIC PURITY OF 2-(AMINOMETHYL)-1-ETHYLPYRROLIDINE
title_fullStr	DEVELOPMENT OF AN EFFICIENT HPLC METHOD WITH PRE-COLUMN DERIVATIZATION FOR DETERMINATION OF ENANTIOMERIC PURITY OF 2-(AMINOMETHYL)-1-ETHYLPYRROLIDINE
title_full_unstemmed	DEVELOPMENT OF AN EFFICIENT HPLC METHOD WITH PRE-COLUMN DERIVATIZATION FOR DETERMINATION OF ENANTIOMERIC PURITY OF 2-(AMINOMETHYL)-1-ETHYLPYRROLIDINE
title_sort	development of an efficient hplc method with pre-column derivatization for determination of enantiomeric purity of 2-(aminomethyl)-1-ethylpyrrolidine
publisher	Sociedad Chilena de Química
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072015000100002
work_keys_str_mv	AT wangdaocai developmentofanefficienthplcmethodwithprecolumnderivatizationfordeterminationofenantiomericpurityof2aminomethyl1ethylpyrrolidine AT yaotian developmentofanefficienthplcmethodwithprecolumnderivatizationfordeterminationofenantiomericpurityof2aminomethyl1ethylpyrrolidine AT qianguofei developmentofanefficienthplcmethodwithprecolumnderivatizationfordeterminationofenantiomericpurityof2aminomethyl1ethylpyrrolidine AT pulingxiang developmentofanefficienthplcmethodwithprecolumnderivatizationfordeterminationofenantiomericpurityof2aminomethyl1ethylpyrrolidine AT yaoshun developmentofanefficienthplcmethodwithprecolumnderivatizationfordeterminationofenantiomericpurityof2aminomethyl1ethylpyrrolidine AT songhang developmentofanefficienthplcmethodwithprecolumnderivatizationfordeterminationofenantiomericpurityof2aminomethyl1ethylpyrrolidine
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DEVELOPMENT OF AN EFFICIENT HPLC METHOD WITH PRE-COLUMN DERIVATIZATION FOR DETERMINATION OF ENANTIOMERIC PURITY OF 2-(AMINOMETHYL)-1-ETHYLPYRROLIDINE

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