PROTECTIVE EFFECT OF PHILESIA MAGELLANICA (COICOPIHUE) FROM CHILEAN PATAGONIA AGAINST OXIDATIVE DAMAGE

Philesia magellanica (P. magellanica) is a plant collected in the Chilean Patagonia. Its antioxidant properties were assessed in human erythrocytes exposed in vitro to oxidative stress induced by HClO. Scanning electron microscopy (SEM) observations showed that HClO induced a morphological alteratio...

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Autores principales:	SUWALSKY,MARIO, AVELLO,MARCIA, OBREQUE,JOSE, VILLENA,FERNANDO, SZYMANSKA,RENATA, STOJAKOWSKA,ANNA, STRZALKA,KAZIMIERZ
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2015
Materias:	Philesia magellanica Coicopihue Plant extract Antioxidant Human erythrocyte Cell membrane
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072015000200016
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spelling	oai:scielo:S0717-970720150002000162019-10-08PROTECTIVE EFFECT OF PHILESIA MAGELLANICA (COICOPIHUE) FROM CHILEAN PATAGONIA AGAINST OXIDATIVE DAMAGESUWALSKY,MARIOAVELLO,MARCIAOBREQUE,JOSEVILLENA,FERNANDOSZYMANSKA,RENATASTOJAKOWSKA,ANNASTRZALKA,KAZIMIERZ Philesia magellanica Coicopihue Plant extract Antioxidant Human erythrocyte Cell membrane Philesia magellanica (P. magellanica) is a plant collected in the Chilean Patagonia. Its antioxidant properties were assessed in human erythrocytes exposed in vitro to oxidative stress induced by HClO. Scanning electron microscopy (SEM) observations showed that HClO induced a morphological alteration in the red blood cells from a normal discoid to a spherocytic form, and cells of unequal size. However, a concentration as low as 1 μM gallic acid equivalents (GAE) of P. magellanica aqueous extract neutralized the change effects of 50 μΜ HClO. On the other hand, 20 μΜ (GAE) of the extract considerably reduced the deleterious capacity of 0.25 mM HClO to induce hemolysis in red blood cells. In addition, X-ray diffraction experiments were performed in molecular models of the human erythrocyte membrane. These consisted in multilayers of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylethanolamine (DMPE), classes of lipids preferentially located in the outer and inner monolayers, respectively of the human erythrocyte membrane. It was observed that P. magellanica only interacted with DMPC affecting its multilayer structure. It was also observed that 0.1 μM (GAE) of P. magellanica neutralized the structural perturbation induced by 0.05 and 0.5 mM HClO. These experiments confirmed the antioxidant properties of P. magellanica aqueous extracts.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.60 n.2 20152015-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072015000200016en10.4067/S0717-97072015000200016
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Philesia magellanica Coicopihue Plant extract Antioxidant Human erythrocyte Cell membrane
spellingShingle	Philesia magellanica Coicopihue Plant extract Antioxidant Human erythrocyte Cell membrane SUWALSKY,MARIO AVELLO,MARCIA OBREQUE,JOSE VILLENA,FERNANDO SZYMANSKA,RENATA STOJAKOWSKA,ANNA STRZALKA,KAZIMIERZ PROTECTIVE EFFECT OF PHILESIA MAGELLANICA (COICOPIHUE) FROM CHILEAN PATAGONIA AGAINST OXIDATIVE DAMAGE
description	Philesia magellanica (P. magellanica) is a plant collected in the Chilean Patagonia. Its antioxidant properties were assessed in human erythrocytes exposed in vitro to oxidative stress induced by HClO. Scanning electron microscopy (SEM) observations showed that HClO induced a morphological alteration in the red blood cells from a normal discoid to a spherocytic form, and cells of unequal size. However, a concentration as low as 1 μM gallic acid equivalents (GAE) of P. magellanica aqueous extract neutralized the change effects of 50 μΜ HClO. On the other hand, 20 μΜ (GAE) of the extract considerably reduced the deleterious capacity of 0.25 mM HClO to induce hemolysis in red blood cells. In addition, X-ray diffraction experiments were performed in molecular models of the human erythrocyte membrane. These consisted in multilayers of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylethanolamine (DMPE), classes of lipids preferentially located in the outer and inner monolayers, respectively of the human erythrocyte membrane. It was observed that P. magellanica only interacted with DMPC affecting its multilayer structure. It was also observed that 0.1 μM (GAE) of P. magellanica neutralized the structural perturbation induced by 0.05 and 0.5 mM HClO. These experiments confirmed the antioxidant properties of P. magellanica aqueous extracts.
author	SUWALSKY,MARIO AVELLO,MARCIA OBREQUE,JOSE VILLENA,FERNANDO SZYMANSKA,RENATA STOJAKOWSKA,ANNA STRZALKA,KAZIMIERZ
author_facet	SUWALSKY,MARIO AVELLO,MARCIA OBREQUE,JOSE VILLENA,FERNANDO SZYMANSKA,RENATA STOJAKOWSKA,ANNA STRZALKA,KAZIMIERZ
author_sort	SUWALSKY,MARIO
title	PROTECTIVE EFFECT OF PHILESIA MAGELLANICA (COICOPIHUE) FROM CHILEAN PATAGONIA AGAINST OXIDATIVE DAMAGE
title_short	PROTECTIVE EFFECT OF PHILESIA MAGELLANICA (COICOPIHUE) FROM CHILEAN PATAGONIA AGAINST OXIDATIVE DAMAGE
title_full	PROTECTIVE EFFECT OF PHILESIA MAGELLANICA (COICOPIHUE) FROM CHILEAN PATAGONIA AGAINST OXIDATIVE DAMAGE
title_fullStr	PROTECTIVE EFFECT OF PHILESIA MAGELLANICA (COICOPIHUE) FROM CHILEAN PATAGONIA AGAINST OXIDATIVE DAMAGE
title_full_unstemmed	PROTECTIVE EFFECT OF PHILESIA MAGELLANICA (COICOPIHUE) FROM CHILEAN PATAGONIA AGAINST OXIDATIVE DAMAGE
title_sort	protective effect of philesia magellanica (coicopihue) from chilean patagonia against oxidative damage
publisher	Sociedad Chilena de Química
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072015000200016
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PROTECTIVE EFFECT OF PHILESIA MAGELLANICA (COICOPIHUE) FROM CHILEAN PATAGONIA AGAINST OXIDATIVE DAMAGE

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