CHARACTERIZATION OF THE ANTIFUNGAL ACTIVITY AGAINST BOTRYTIS CINEREA OF SCLAREOL AND 13-EPI-SCLAREOL, TWO LABDANE-TYPE DITERPENOIDS

The antifungal activity of Sclareol and 13-epi-Sclareol, two labdane-type diterpenoids, on mycelial growth of the phytopathogenic fungus Botrytis cinerea was evaluated. Diterpenoid fungitoxicity was assessed using the radial growth test method. Both diterpenoids inhibited the mycelial growth of B. c...

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Autores principales:	MENDOZA,LEONORA, SEPÚLVEDA,CAROLINA, MELO,RICARDO, COTORAS,MILENA
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2015
Materias:	Sclareol 13-epi-Sclareol antifungal activity Botrytis cinerea
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072015000300010
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spelling	oai:scielo:S0717-970720150003000102015-12-04CHARACTERIZATION OF THE ANTIFUNGAL ACTIVITY AGAINST BOTRYTIS CINEREA OF SCLAREOL AND 13-EPI-SCLAREOL, TWO LABDANE-TYPE DITERPENOIDSMENDOZA,LEONORASEPÚLVEDA,CAROLINAMELO,RICARDOCOTORAS,MILENA Sclareol 13-epi-Sclareol antifungal activity Botrytis cinerea The antifungal activity of Sclareol and 13-epi-Sclareol, two labdane-type diterpenoids, on mycelial growth of the phytopathogenic fungus Botrytis cinerea was evaluated. Diterpenoid fungitoxicity was assessed using the radial growth test method. Both diterpenoids inhibited the mycelial growth of B. cinerea in solid medium; however the inhibitory activity of Sclareol was slightly higher than 13-epi-Sclareol with IC50 value of 237.6 μg/mL and 268.8 μg/mL, respectively. On the other hand, both labdane-type diterpenoids did not alter the plasmatic membrane integrity; however the oxygen consumption of B. cinerea germinating conidia was affected. The evidence suggests that action mechanism of these molecules would be related to the uncoupling of mitochondrial oxidative phosphorylation. Finally, biotransformation of Sclareol by B. cinerea was analysed and the main biotransformed metabolite was identified as 3ß-hydroxysclareol.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.60 n.3 20152015-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072015000300010en10.4067/S0717-97072015000300010
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Sclareol 13-epi-Sclareol antifungal activity Botrytis cinerea
spellingShingle	Sclareol 13-epi-Sclareol antifungal activity Botrytis cinerea MENDOZA,LEONORA SEPÚLVEDA,CAROLINA MELO,RICARDO COTORAS,MILENA CHARACTERIZATION OF THE ANTIFUNGAL ACTIVITY AGAINST BOTRYTIS CINEREA OF SCLAREOL AND 13-EPI-SCLAREOL, TWO LABDANE-TYPE DITERPENOIDS
description	The antifungal activity of Sclareol and 13-epi-Sclareol, two labdane-type diterpenoids, on mycelial growth of the phytopathogenic fungus Botrytis cinerea was evaluated. Diterpenoid fungitoxicity was assessed using the radial growth test method. Both diterpenoids inhibited the mycelial growth of B. cinerea in solid medium; however the inhibitory activity of Sclareol was slightly higher than 13-epi-Sclareol with IC50 value of 237.6 μg/mL and 268.8 μg/mL, respectively. On the other hand, both labdane-type diterpenoids did not alter the plasmatic membrane integrity; however the oxygen consumption of B. cinerea germinating conidia was affected. The evidence suggests that action mechanism of these molecules would be related to the uncoupling of mitochondrial oxidative phosphorylation. Finally, biotransformation of Sclareol by B. cinerea was analysed and the main biotransformed metabolite was identified as 3ß-hydroxysclareol.
author	MENDOZA,LEONORA SEPÚLVEDA,CAROLINA MELO,RICARDO COTORAS,MILENA
author_facet	MENDOZA,LEONORA SEPÚLVEDA,CAROLINA MELO,RICARDO COTORAS,MILENA
author_sort	MENDOZA,LEONORA
title	CHARACTERIZATION OF THE ANTIFUNGAL ACTIVITY AGAINST BOTRYTIS CINEREA OF SCLAREOL AND 13-EPI-SCLAREOL, TWO LABDANE-TYPE DITERPENOIDS
title_short	CHARACTERIZATION OF THE ANTIFUNGAL ACTIVITY AGAINST BOTRYTIS CINEREA OF SCLAREOL AND 13-EPI-SCLAREOL, TWO LABDANE-TYPE DITERPENOIDS
title_full	CHARACTERIZATION OF THE ANTIFUNGAL ACTIVITY AGAINST BOTRYTIS CINEREA OF SCLAREOL AND 13-EPI-SCLAREOL, TWO LABDANE-TYPE DITERPENOIDS
title_fullStr	CHARACTERIZATION OF THE ANTIFUNGAL ACTIVITY AGAINST BOTRYTIS CINEREA OF SCLAREOL AND 13-EPI-SCLAREOL, TWO LABDANE-TYPE DITERPENOIDS
title_full_unstemmed	CHARACTERIZATION OF THE ANTIFUNGAL ACTIVITY AGAINST BOTRYTIS CINEREA OF SCLAREOL AND 13-EPI-SCLAREOL, TWO LABDANE-TYPE DITERPENOIDS
title_sort	characterization of the antifungal activity against botrytis cinerea of sclareol and 13-epi-sclareol, two labdane-type diterpenoids
publisher	Sociedad Chilena de Química
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072015000300010
work_keys_str_mv	AT mendozaleonora characterizationoftheantifungalactivityagainstbotrytiscinereaofsclareoland13episclareoltwolabdanetypediterpenoids AT sepulvedacarolina characterizationoftheantifungalactivityagainstbotrytiscinereaofsclareoland13episclareoltwolabdanetypediterpenoids AT meloricardo characterizationoftheantifungalactivityagainstbotrytiscinereaofsclareoland13episclareoltwolabdanetypediterpenoids AT cotorasmilena characterizationoftheantifungalactivityagainstbotrytiscinereaofsclareoland13episclareoltwolabdanetypediterpenoids
_version_	1718445539338485760

CHARACTERIZATION OF THE ANTIFUNGAL ACTIVITY AGAINST BOTRYTIS CINEREA OF SCLAREOL AND 13-EPI-SCLAREOL, TWO LABDANE-TYPE DITERPENOIDS

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