DEVELOPMENT OF A SIMPLE AND SPECIFIC UHPLC ASSAY FOR THE DETERMINATION OF ARIPIPRAZOLE AND DEHYDROARDPDPRAZOLE IN RAT PLASMA

A new, simple, specific, and rapid ultra high performance liquid chromatography method was developed and validated for the determination of aripiprazole and its active metabolite dehydroaripiprazole in rat plasma. The analysis was performed on a Zorbax Eclipse Plus C18 (50 mm × 2.1 mm, 1.8 &#956...

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Autores principales:	YENICELI UĞUR,DUYGU, YILMAZ,IŞIL T, ŞENER,EROL
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2015
Materias:	Aripiprazole dehydroaripiprazole UHPLC rat plasma validation protein precipitation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072015000300016
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spelling	oai:scielo:S0717-970720150003000162015-12-04DEVELOPMENT OF A SIMPLE AND SPECIFIC UHPLC ASSAY FOR THE DETERMINATION OF ARIPIPRAZOLE AND DEHYDROARDPDPRAZOLE IN RAT PLASMAYENICELI UĞUR,DUYGUYILMAZ,IŞIL TŞENER,EROL Aripiprazole dehydroaripiprazole UHPLC rat plasma validation protein precipitation A new, simple, specific, and rapid ultra high performance liquid chromatography method was developed and validated for the determination of aripiprazole and its active metabolite dehydroaripiprazole in rat plasma. The analysis was performed on a Zorbax Eclipse Plus C18 (50 mm × 2.1 mm, 1.8 μm particles) column using gradient elution with a mobile phase consisting of acetonitrile and acetate buffer (30 mM, pH 5). Plasma samples were analyzed after a simple, one-step protein precipitation with acetonitrile. The method was validated and the specificity, linearity, limit of detection, limit of quantitation, precision, accuracy, recoveries, matrix effect, stability and robustness were determined. The analytes and internal standard were separated in 3.5 min and the total analysis time including the clean-up step was 8 min. Limit of detection was 0.012 μg mL-1 and 0.009 μg mL-1 for aripiprazole and dehydroaripiprazole, respectively. Limit of quantitation was 0.039 μg mL-1 and 0.029 μg mL-1 for aripiprazole and dehydroaripiprazole, respectively. The intra- and inter-day assay variability was less than 2% for the analytes. The proposed method is rapid, reproducible and accurate to quantify both aripiprazole and dehydroaripiprazole. It involves a simple plasma deproteination technique using one step protein precipitation with acetonitrile. This validated method was successfully used to quantify plasma concentrations of the analytes in rat plasma.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.60 n.3 20152015-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072015000300016en10.4067/S0717-97072015000300016
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Aripiprazole dehydroaripiprazole UHPLC rat plasma validation protein precipitation
spellingShingle	Aripiprazole dehydroaripiprazole UHPLC rat plasma validation protein precipitation YENICELI UĞUR,DUYGU YILMAZ,IŞIL T ŞENER,EROL DEVELOPMENT OF A SIMPLE AND SPECIFIC UHPLC ASSAY FOR THE DETERMINATION OF ARIPIPRAZOLE AND DEHYDROARDPDPRAZOLE IN RAT PLASMA
description	A new, simple, specific, and rapid ultra high performance liquid chromatography method was developed and validated for the determination of aripiprazole and its active metabolite dehydroaripiprazole in rat plasma. The analysis was performed on a Zorbax Eclipse Plus C18 (50 mm × 2.1 mm, 1.8 μm particles) column using gradient elution with a mobile phase consisting of acetonitrile and acetate buffer (30 mM, pH 5). Plasma samples were analyzed after a simple, one-step protein precipitation with acetonitrile. The method was validated and the specificity, linearity, limit of detection, limit of quantitation, precision, accuracy, recoveries, matrix effect, stability and robustness were determined. The analytes and internal standard were separated in 3.5 min and the total analysis time including the clean-up step was 8 min. Limit of detection was 0.012 μg mL-1 and 0.009 μg mL-1 for aripiprazole and dehydroaripiprazole, respectively. Limit of quantitation was 0.039 μg mL-1 and 0.029 μg mL-1 for aripiprazole and dehydroaripiprazole, respectively. The intra- and inter-day assay variability was less than 2% for the analytes. The proposed method is rapid, reproducible and accurate to quantify both aripiprazole and dehydroaripiprazole. It involves a simple plasma deproteination technique using one step protein precipitation with acetonitrile. This validated method was successfully used to quantify plasma concentrations of the analytes in rat plasma.
author	YENICELI UĞUR,DUYGU YILMAZ,IŞIL T ŞENER,EROL
author_facet	YENICELI UĞUR,DUYGU YILMAZ,IŞIL T ŞENER,EROL
author_sort	YENICELI UĞUR,DUYGU
title	DEVELOPMENT OF A SIMPLE AND SPECIFIC UHPLC ASSAY FOR THE DETERMINATION OF ARIPIPRAZOLE AND DEHYDROARDPDPRAZOLE IN RAT PLASMA
title_short	DEVELOPMENT OF A SIMPLE AND SPECIFIC UHPLC ASSAY FOR THE DETERMINATION OF ARIPIPRAZOLE AND DEHYDROARDPDPRAZOLE IN RAT PLASMA
title_full	DEVELOPMENT OF A SIMPLE AND SPECIFIC UHPLC ASSAY FOR THE DETERMINATION OF ARIPIPRAZOLE AND DEHYDROARDPDPRAZOLE IN RAT PLASMA
title_fullStr	DEVELOPMENT OF A SIMPLE AND SPECIFIC UHPLC ASSAY FOR THE DETERMINATION OF ARIPIPRAZOLE AND DEHYDROARDPDPRAZOLE IN RAT PLASMA
title_full_unstemmed	DEVELOPMENT OF A SIMPLE AND SPECIFIC UHPLC ASSAY FOR THE DETERMINATION OF ARIPIPRAZOLE AND DEHYDROARDPDPRAZOLE IN RAT PLASMA
title_sort	development of a simple and specific uhplc assay for the determination of aripiprazole and dehydroardpdprazole in rat plasma
publisher	Sociedad Chilena de Química
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072015000300016
work_keys_str_mv	AT yeniceliu286urduygu developmentofasimpleandspecificuhplcassayforthedeterminationofaripiprazoleanddehydroardpdprazoleinratplasma AT yilmazi350ilt developmentofasimpleandspecificuhplcassayforthedeterminationofaripiprazoleanddehydroardpdprazoleinratplasma AT 350enererol developmentofasimpleandspecificuhplcassayforthedeterminationofaripiprazoleanddehydroardpdprazoleinratplasma
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DEVELOPMENT OF A SIMPLE AND SPECIFIC UHPLC ASSAY FOR THE DETERMINATION OF ARIPIPRAZOLE AND DEHYDROARDPDPRAZOLE IN RAT PLASMA

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