VALIDATED THIN-LAYER CHROMATOGRAPHY/DENSITOMETRY METHOD FOR THE ANALYSIS OF ANTI-ALZHEIMER DRUG IN BULK AND IN CAPSULE FORMULATION

A simple, sensitive and precise Thin-Layer Chromatography (TLC)/ Densitometry method has been developed for estimation of Rivastigmine hydrogen tartrate [RVT] in bulk and in capsule formulation. The chromatographic separation was accomplished on aluminium backed precoated silica gel 60 F254 as the s...

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Autores principales:	KHAN,MOHAMMAD MOJEEB GULZAR, SHIRKHEDKAR,ATUL ARUN
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2015
Materias:	Rivastigmine hydrogen tartrate RVT HPTLC Stability-indicating Validation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072015000400005
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spelling	oai:scielo:S0717-970720150004000052016-02-23VALIDATED THIN-LAYER CHROMATOGRAPHY/DENSITOMETRY METHOD FOR THE ANALYSIS OF ANTI-ALZHEIMER DRUG IN BULK AND IN CAPSULE FORMULATIONKHAN,MOHAMMAD MOJEEB GULZARSHIRKHEDKAR,ATUL ARUN Rivastigmine hydrogen tartrate RVT HPTLC Stability-indicating Validation A simple, sensitive and precise Thin-Layer Chromatography (TLC)/ Densitometry method has been developed for estimation of Rivastigmine hydrogen tartrate [RVT] in bulk and in capsule formulation. The chromatographic separation was accomplished on aluminium backed precoated silica gel 60 F254 as the stationary phase using n- Hexane: Ethyl acetate: triethylamine (1.5: 8.5: 0.3 v/v) as mobile phase. The R F value was observed to be 0.46 ± 0.02 for RVT when the sports were analyzed densitometrically at 213 nm. The method was linear in the range of 1000 - 6000 ng band-1 with coefficient correlation value 0.9990. RVT was subjected to acid, alkali hydrolysis, oxidation, dry heat degradation and photo degradation. The degraded product peaks were well resolved from the pure drug peak with significant difference in their R F values. Analysis of drug was performed in the presence of degraded samples. The proposed method validated for linearity, accuracy, precision and robustness. The developed TLC-densitometric method can be used as an alternative method for the precise assay of rivastigmine hydrogen tartrate in capsules form because it fulfils the validation criteria of an analytical method designated for quantity control of pharmaceutical preparations.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.60 n.4 20152015-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072015000400005en10.4067/S0717-97072015000400005
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Rivastigmine hydrogen tartrate RVT HPTLC Stability-indicating Validation
spellingShingle	Rivastigmine hydrogen tartrate RVT HPTLC Stability-indicating Validation KHAN,MOHAMMAD MOJEEB GULZAR SHIRKHEDKAR,ATUL ARUN VALIDATED THIN-LAYER CHROMATOGRAPHY/DENSITOMETRY METHOD FOR THE ANALYSIS OF ANTI-ALZHEIMER DRUG IN BULK AND IN CAPSULE FORMULATION
description	A simple, sensitive and precise Thin-Layer Chromatography (TLC)/ Densitometry method has been developed for estimation of Rivastigmine hydrogen tartrate [RVT] in bulk and in capsule formulation. The chromatographic separation was accomplished on aluminium backed precoated silica gel 60 F254 as the stationary phase using n- Hexane: Ethyl acetate: triethylamine (1.5: 8.5: 0.3 v/v) as mobile phase. The R F value was observed to be 0.46 ± 0.02 for RVT when the sports were analyzed densitometrically at 213 nm. The method was linear in the range of 1000 - 6000 ng band-1 with coefficient correlation value 0.9990. RVT was subjected to acid, alkali hydrolysis, oxidation, dry heat degradation and photo degradation. The degraded product peaks were well resolved from the pure drug peak with significant difference in their R F values. Analysis of drug was performed in the presence of degraded samples. The proposed method validated for linearity, accuracy, precision and robustness. The developed TLC-densitometric method can be used as an alternative method for the precise assay of rivastigmine hydrogen tartrate in capsules form because it fulfils the validation criteria of an analytical method designated for quantity control of pharmaceutical preparations.
author	KHAN,MOHAMMAD MOJEEB GULZAR SHIRKHEDKAR,ATUL ARUN
author_facet	KHAN,MOHAMMAD MOJEEB GULZAR SHIRKHEDKAR,ATUL ARUN
author_sort	KHAN,MOHAMMAD MOJEEB GULZAR
title	VALIDATED THIN-LAYER CHROMATOGRAPHY/DENSITOMETRY METHOD FOR THE ANALYSIS OF ANTI-ALZHEIMER DRUG IN BULK AND IN CAPSULE FORMULATION
title_short	VALIDATED THIN-LAYER CHROMATOGRAPHY/DENSITOMETRY METHOD FOR THE ANALYSIS OF ANTI-ALZHEIMER DRUG IN BULK AND IN CAPSULE FORMULATION
title_full	VALIDATED THIN-LAYER CHROMATOGRAPHY/DENSITOMETRY METHOD FOR THE ANALYSIS OF ANTI-ALZHEIMER DRUG IN BULK AND IN CAPSULE FORMULATION
title_fullStr	VALIDATED THIN-LAYER CHROMATOGRAPHY/DENSITOMETRY METHOD FOR THE ANALYSIS OF ANTI-ALZHEIMER DRUG IN BULK AND IN CAPSULE FORMULATION
title_full_unstemmed	VALIDATED THIN-LAYER CHROMATOGRAPHY/DENSITOMETRY METHOD FOR THE ANALYSIS OF ANTI-ALZHEIMER DRUG IN BULK AND IN CAPSULE FORMULATION
title_sort	validated thin-layer chromatography/densitometry method for the analysis of anti-alzheimer drug in bulk and in capsule formulation
publisher	Sociedad Chilena de Química
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072015000400005
work_keys_str_mv	AT khanmohammadmojeebgulzar validatedthinlayerchromatographydensitometrymethodfortheanalysisofantialzheimerdruginbulkandincapsuleformulation AT shirkhedkaratularun validatedthinlayerchromatographydensitometrymethodfortheanalysisofantialzheimerdruginbulkandincapsuleformulation
_version_	1718445542543982592

VALIDATED THIN-LAYER CHROMATOGRAPHY/DENSITOMETRY METHOD FOR THE ANALYSIS OF ANTI-ALZHEIMER DRUG IN BULK AND IN CAPSULE FORMULATION

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