TEMPERATURE EFFECT ON BINDING AFFINITY AND STOICHIOMETRY BETWEEN SOME STEROIDS AND HUMAN SERUM ALBUMIN
The temperature dependence of the interactions between some steroids (fusidic acid, ouabain and ethynodioldiacetate) and human serum albumin (HSA) at physiological pH (7.4) were studied systematically by square-wave voltammetry. Also, these interactions were proven by means of UV-Vis. absorptionspec...
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| Autores principales: | , |
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| Lenguaje: | English |
| Publicado: |
Sociedad Chilena de Química
2016
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| Materias: | |
| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072016000100013 |
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| Sumario: | The temperature dependence of the interactions between some steroids (fusidic acid, ouabain and ethynodioldiacetate) and human serum albumin (HSA) at physiological pH (7.4) were studied systematically by square-wave voltammetry. Also, these interactions were proven by means of UV-Vis. absorptionspectroscopy and FT-IR spectroscopy techniques. The interaction parameters (binding constants and stoichiometries) at temperature range of 297.5-312.5 K weredetermined from electrochemical data. It is worthy that the temperature has played a positive role on the interactions of fusidic acid with HSA; however it hasnegative effective on the interaction parameters of HSA with ouabain or ethynodioldiacetate. |
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