PHENOLIC COMPOSITION AND BIOLOGICAL (ANTI DIABETIC AND ANTIOXIDANT) ACTIVITIES OF DIFFERENT SOLVENT EXTRACTS OF AN ENDEMIC PLANT (HELIOTROPIUM STRIGOSUM)

Heliotropium strigosum plant is widely used in traditional medicines for treatment of various ailments. In current study, an effort was made to evalúate phenolic profile as well as antidiabetic and antioxidant activities of crude methanol extract and different solvent (n-hexane, ethyl acetate, chlor...

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Autores principales:	QAYYUM,ABDUL, SARFRAZ,RAJA ADIL, ASHRAF,AISHA, ADIL,SHAZIA
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2016
Materias:	Antidiabetic DPPH Heliotropium Strigosum Solvent fractions
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072016000100016
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spelling	oai:scielo:S0717-970720160001000162016-09-14PHENOLIC COMPOSITION AND BIOLOGICAL (ANTI DIABETIC AND ANTIOXIDANT) ACTIVITIES OF DIFFERENT SOLVENT EXTRACTS OF AN ENDEMIC PLANT (HELIOTROPIUM STRIGOSUM)QAYYUM,ABDULSARFRAZ,RAJA ADILASHRAF,AISHAADIL,SHAZIA Antidiabetic DPPH Heliotropium Strigosum Solvent fractions Heliotropium strigosum plant is widely used in traditional medicines for treatment of various ailments. In current study, an effort was made to evalúate phenolic profile as well as antidiabetic and antioxidant activities of crude methanol extract and different solvent (n-hexane, ethyl acetate, chloroform and aqueous) fractions of H. strigosum. Total phenolic contents were determined by spectrophotometric assay. Polyphenolic compounds in crude methanol extract and each solvent fraction were identified by reverse phase high performance liquid chromatography (RP-HPLC). Antioxidant and antidiabetic activities were determined using DPPH and a-amylase inhibition assays, respectively. The outcome of spectrophotometric assay showed that methanol extract had higher amount of total phenolic (84.50 ± 0.06 pg GAE/ mg of plant extract) contents than aqueous, ethyl acetate, chloroform and n-hexane fractions, respectively. The RP-HPLC analysis revealed the maximum number of phenolic (chromotropic acid, quercetin, trans-4-hydroxy-3-methoxy cinnamic acid, vanillic acid, gallic acid, caffeic acid, m-coumaric acid, p-coumaric acid, syringic acid, sinapic acid and ferulic acid) compounds in methanol extract. Methanol extract (IC5(0) = 8.97 pg/mL) exhibited the maximum antidiabetic activity followed by aqueous (IC50 = 20.04 pg/mL), ethyl acetate (IC50 = 27.79 pg/mL), chloroform (IC50 = 56.87 pg/mL) and hexane (IC50 = 32.16 pg/mL) fractions, respectively. The outcome of antioxidant assay revealed that methanol extract was the leading one with regard to antioxidant activity at different doses (10 to 250 pg/mL). The current study concludes that H. strigosum solvents extracts with significant phenolic profile and potent biological activities could be explored for potential uses in neutraceutical and pharmaceutical industries.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.61 n.1 20162016-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072016000100016en10.4067/S0717-97072016000100016
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Antidiabetic DPPH Heliotropium Strigosum Solvent fractions
spellingShingle	Antidiabetic DPPH Heliotropium Strigosum Solvent fractions QAYYUM,ABDUL SARFRAZ,RAJA ADIL ASHRAF,AISHA ADIL,SHAZIA PHENOLIC COMPOSITION AND BIOLOGICAL (ANTI DIABETIC AND ANTIOXIDANT) ACTIVITIES OF DIFFERENT SOLVENT EXTRACTS OF AN ENDEMIC PLANT (HELIOTROPIUM STRIGOSUM)
description	Heliotropium strigosum plant is widely used in traditional medicines for treatment of various ailments. In current study, an effort was made to evalúate phenolic profile as well as antidiabetic and antioxidant activities of crude methanol extract and different solvent (n-hexane, ethyl acetate, chloroform and aqueous) fractions of H. strigosum. Total phenolic contents were determined by spectrophotometric assay. Polyphenolic compounds in crude methanol extract and each solvent fraction were identified by reverse phase high performance liquid chromatography (RP-HPLC). Antioxidant and antidiabetic activities were determined using DPPH and a-amylase inhibition assays, respectively. The outcome of spectrophotometric assay showed that methanol extract had higher amount of total phenolic (84.50 ± 0.06 pg GAE/ mg of plant extract) contents than aqueous, ethyl acetate, chloroform and n-hexane fractions, respectively. The RP-HPLC analysis revealed the maximum number of phenolic (chromotropic acid, quercetin, trans-4-hydroxy-3-methoxy cinnamic acid, vanillic acid, gallic acid, caffeic acid, m-coumaric acid, p-coumaric acid, syringic acid, sinapic acid and ferulic acid) compounds in methanol extract. Methanol extract (IC5(0) = 8.97 pg/mL) exhibited the maximum antidiabetic activity followed by aqueous (IC50 = 20.04 pg/mL), ethyl acetate (IC50 = 27.79 pg/mL), chloroform (IC50 = 56.87 pg/mL) and hexane (IC50 = 32.16 pg/mL) fractions, respectively. The outcome of antioxidant assay revealed that methanol extract was the leading one with regard to antioxidant activity at different doses (10 to 250 pg/mL). The current study concludes that H. strigosum solvents extracts with significant phenolic profile and potent biological activities could be explored for potential uses in neutraceutical and pharmaceutical industries.
author	QAYYUM,ABDUL SARFRAZ,RAJA ADIL ASHRAF,AISHA ADIL,SHAZIA
author_facet	QAYYUM,ABDUL SARFRAZ,RAJA ADIL ASHRAF,AISHA ADIL,SHAZIA
author_sort	QAYYUM,ABDUL
title	PHENOLIC COMPOSITION AND BIOLOGICAL (ANTI DIABETIC AND ANTIOXIDANT) ACTIVITIES OF DIFFERENT SOLVENT EXTRACTS OF AN ENDEMIC PLANT (HELIOTROPIUM STRIGOSUM)
title_short	PHENOLIC COMPOSITION AND BIOLOGICAL (ANTI DIABETIC AND ANTIOXIDANT) ACTIVITIES OF DIFFERENT SOLVENT EXTRACTS OF AN ENDEMIC PLANT (HELIOTROPIUM STRIGOSUM)
title_full	PHENOLIC COMPOSITION AND BIOLOGICAL (ANTI DIABETIC AND ANTIOXIDANT) ACTIVITIES OF DIFFERENT SOLVENT EXTRACTS OF AN ENDEMIC PLANT (HELIOTROPIUM STRIGOSUM)
title_fullStr	PHENOLIC COMPOSITION AND BIOLOGICAL (ANTI DIABETIC AND ANTIOXIDANT) ACTIVITIES OF DIFFERENT SOLVENT EXTRACTS OF AN ENDEMIC PLANT (HELIOTROPIUM STRIGOSUM)
title_full_unstemmed	PHENOLIC COMPOSITION AND BIOLOGICAL (ANTI DIABETIC AND ANTIOXIDANT) ACTIVITIES OF DIFFERENT SOLVENT EXTRACTS OF AN ENDEMIC PLANT (HELIOTROPIUM STRIGOSUM)
title_sort	phenolic composition and biological (anti diabetic and antioxidant) activities of different solvent extracts of an endemic plant (heliotropium strigosum)
publisher	Sociedad Chilena de Química
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072016000100016
work_keys_str_mv	AT qayyumabdul phenoliccompositionandbiologicalantidiabeticandantioxidantactivitiesofdifferentsolventextractsofanendemicplantheliotropiumstrigosum AT sarfrazrajaadil phenoliccompositionandbiologicalantidiabeticandantioxidantactivitiesofdifferentsolventextractsofanendemicplantheliotropiumstrigosum AT ashrafaisha phenoliccompositionandbiologicalantidiabeticandantioxidantactivitiesofdifferentsolventextractsofanendemicplantheliotropiumstrigosum AT adilshazia phenoliccompositionandbiologicalantidiabeticandantioxidantactivitiesofdifferentsolventextractsofanendemicplantheliotropiumstrigosum
_version_	1718445548550225920

PHENOLIC COMPOSITION AND BIOLOGICAL (ANTI DIABETIC AND ANTIOXIDANT) ACTIVITIES OF DIFFERENT SOLVENT EXTRACTS OF AN ENDEMIC PLANT (HELIOTROPIUM STRIGOSUM)

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