DEVELOPMENT AND VALIDATION OF RP- HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF TRAMADOL HYDROCHLORIDE AND DICYCLOMINE IN BULK AND PHARMACEUTICAL FORMULATION

A simple, specific, accurate and precise reversed phase high pressure liquid chromatography (RP-HPLC) method has been developed for the simultaneous estimation of tramadol hydrochloride (TRA) and dicyclomine (DCY) in bulk and tablet dosage form. Chromatography was carried on Phenomex Gemini C column...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	DONDA,SHAILESH T, BAVISKAR,VISHAL B, BARI,SANJAY B, DESHMUKH,PRASHANT K, DEORE,DARSHAN S, GIRASE,NAYANDIP M, KHAN,ZAMIR G, PATIL,PRAVIN O
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2016
Materias:	Dicyclomine RP-HPLC Tramadol hydrochloride
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072016000200001
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-97072016000200001
record_format	dspace
spelling	oai:scielo:S0717-970720160002000012016-09-14DEVELOPMENT AND VALIDATION OF RP- HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF TRAMADOL HYDROCHLORIDE AND DICYCLOMINE IN BULK AND PHARMACEUTICAL FORMULATIONDONDA,SHAILESH TBAVISKAR,VISHAL BBARI,SANJAY BDESHMUKH,PRASHANT KDEORE,DARSHAN SGIRASE,NAYANDIP MKHAN,ZAMIR GPATIL,PRAVIN O Dicyclomine RP-HPLC Tramadol hydrochloride A simple, specific, accurate and precise reversed phase high pressure liquid chromatography (RP-HPLC) method has been developed for the simultaneous estimation of tramadol hydrochloride (TRA) and dicyclomine (DCY) in bulk and tablet dosage form. Chromatography was carried on Phenomex Gemini C column (4.6 x 250 mm, 5u particle size) using mixture of methanol, acetonitrile and 0.1% triethylamine (TEA) pH 3.0 (adjusted with orthophophoric acid) in the ratio of 35:5:60 (v/v/v) respectively as a mobile phase. The flow rate 1.0mL min-1 and detection wavelength 275 nm was selected for the present study. The retention time of tramadol hydrochloride and dicyclomine were 4.13 min and 9.06 min respectively. The linear regression analysis data for the calibration plots showed a good linear relationship over the concentration range of 50-300 μg mL-1 for tramadol hydrochloride and 10-60 μg mL-1 for dicyclomine. The proposed method was validated with respect to linearity, precision, accuracy, specificity and robustness. The proposed method was found to be simple, rapid, accurate, precise and reproducible. Thus it can be applied for routine quality control analysis of tramadol hydrochloride and dicyclomine in tablet dosage form.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.61 n.2 20162016-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072016000200001en10.4067/S0717-97072016000200001
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Dicyclomine RP-HPLC Tramadol hydrochloride
spellingShingle	Dicyclomine RP-HPLC Tramadol hydrochloride DONDA,SHAILESH T BAVISKAR,VISHAL B BARI,SANJAY B DESHMUKH,PRASHANT K DEORE,DARSHAN S GIRASE,NAYANDIP M KHAN,ZAMIR G PATIL,PRAVIN O DEVELOPMENT AND VALIDATION OF RP- HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF TRAMADOL HYDROCHLORIDE AND DICYCLOMINE IN BULK AND PHARMACEUTICAL FORMULATION
description	A simple, specific, accurate and precise reversed phase high pressure liquid chromatography (RP-HPLC) method has been developed for the simultaneous estimation of tramadol hydrochloride (TRA) and dicyclomine (DCY) in bulk and tablet dosage form. Chromatography was carried on Phenomex Gemini C column (4.6 x 250 mm, 5u particle size) using mixture of methanol, acetonitrile and 0.1% triethylamine (TEA) pH 3.0 (adjusted with orthophophoric acid) in the ratio of 35:5:60 (v/v/v) respectively as a mobile phase. The flow rate 1.0mL min-1 and detection wavelength 275 nm was selected for the present study. The retention time of tramadol hydrochloride and dicyclomine were 4.13 min and 9.06 min respectively. The linear regression analysis data for the calibration plots showed a good linear relationship over the concentration range of 50-300 μg mL-1 for tramadol hydrochloride and 10-60 μg mL-1 for dicyclomine. The proposed method was validated with respect to linearity, precision, accuracy, specificity and robustness. The proposed method was found to be simple, rapid, accurate, precise and reproducible. Thus it can be applied for routine quality control analysis of tramadol hydrochloride and dicyclomine in tablet dosage form.
author	DONDA,SHAILESH T BAVISKAR,VISHAL B BARI,SANJAY B DESHMUKH,PRASHANT K DEORE,DARSHAN S GIRASE,NAYANDIP M KHAN,ZAMIR G PATIL,PRAVIN O
author_facet	DONDA,SHAILESH T BAVISKAR,VISHAL B BARI,SANJAY B DESHMUKH,PRASHANT K DEORE,DARSHAN S GIRASE,NAYANDIP M KHAN,ZAMIR G PATIL,PRAVIN O
author_sort	DONDA,SHAILESH T
title	DEVELOPMENT AND VALIDATION OF RP- HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF TRAMADOL HYDROCHLORIDE AND DICYCLOMINE IN BULK AND PHARMACEUTICAL FORMULATION
title_short	DEVELOPMENT AND VALIDATION OF RP- HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF TRAMADOL HYDROCHLORIDE AND DICYCLOMINE IN BULK AND PHARMACEUTICAL FORMULATION
title_full	DEVELOPMENT AND VALIDATION OF RP- HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF TRAMADOL HYDROCHLORIDE AND DICYCLOMINE IN BULK AND PHARMACEUTICAL FORMULATION
title_fullStr	DEVELOPMENT AND VALIDATION OF RP- HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF TRAMADOL HYDROCHLORIDE AND DICYCLOMINE IN BULK AND PHARMACEUTICAL FORMULATION
title_full_unstemmed	DEVELOPMENT AND VALIDATION OF RP- HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF TRAMADOL HYDROCHLORIDE AND DICYCLOMINE IN BULK AND PHARMACEUTICAL FORMULATION
title_sort	development and validation of rp- hplc method for the simultaneous estimation of tramadol hydrochloride and dicyclomine in bulk and pharmaceutical formulation
publisher	Sociedad Chilena de Química
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072016000200001
work_keys_str_mv	AT dondashailesht developmentandvalidationofrphplcmethodforthesimultaneousestimationoftramadolhydrochlorideanddicyclomineinbulkandpharmaceuticalformulation AT baviskarvishalb developmentandvalidationofrphplcmethodforthesimultaneousestimationoftramadolhydrochlorideanddicyclomineinbulkandpharmaceuticalformulation AT barisanjayb developmentandvalidationofrphplcmethodforthesimultaneousestimationoftramadolhydrochlorideanddicyclomineinbulkandpharmaceuticalformulation AT deshmukhprashantk developmentandvalidationofrphplcmethodforthesimultaneousestimationoftramadolhydrochlorideanddicyclomineinbulkandpharmaceuticalformulation AT deoredarshans developmentandvalidationofrphplcmethodforthesimultaneousestimationoftramadolhydrochlorideanddicyclomineinbulkandpharmaceuticalformulation AT girasenayandipm developmentandvalidationofrphplcmethodforthesimultaneousestimationoftramadolhydrochlorideanddicyclomineinbulkandpharmaceuticalformulation AT khanzamirg developmentandvalidationofrphplcmethodforthesimultaneousestimationoftramadolhydrochlorideanddicyclomineinbulkandpharmaceuticalformulation AT patilpravino developmentandvalidationofrphplcmethodforthesimultaneousestimationoftramadolhydrochlorideanddicyclomineinbulkandpharmaceuticalformulation
_version_	1718445549274791936

DEVELOPMENT AND VALIDATION OF RP- HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF TRAMADOL HYDROCHLORIDE AND DICYCLOMINE IN BULK AND PHARMACEUTICAL FORMULATION

Ejemplares similares