REVERSE PHASE HPLC AND DERIVATIVE SPECTROPHOTOMETRIC METHODS FOR SIMULTANEOUS ESTIMATION OF FENBENDAZOLE AND NICLOSAMIDE IN PHARMACEUTICAL DOSAGE FORM

The present study narrates the developed and validated simple, reliable, sensitive, precise and accurate Spectrophotometric and RP-HPLC methods for the simultaneous estimation of Fenbendazole and Niclosamide in pharmaceutical dosage form. In the first order derivative method 0.1 N methanolic HCl was...

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Autores principales: SHAH,UMANG, GANDHI,AYUSHI, DAWAWALA,TRUPTI
Lenguaje:English
Publicado: Sociedad Chilena de Química 2016
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072016000200030
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spelling oai:scielo:S0717-970720160002000302016-09-14REVERSE PHASE HPLC AND DERIVATIVE SPECTROPHOTOMETRIC METHODS FOR SIMULTANEOUS ESTIMATION OF FENBENDAZOLE AND NICLOSAMIDE IN PHARMACEUTICAL DOSAGE FORMSHAH,UMANGGANDHI,AYUSHIDAWAWALA,TRUPTI Fenbendazole Niclosamide UV- Spectrophotometry RP-HPLC Validation The present study narrates the developed and validated simple, reliable, sensitive, precise and accurate Spectrophotometric and RP-HPLC methods for the simultaneous estimation of Fenbendazole and Niclosamide in pharmaceutical dosage form. In the first order derivative method 0.1 N methanolic HCl was used as diluent. The zero crossing point wavelengths selected for the analysis were 226 nm and 317 nm for Fenbendazole and Niclosamide, respectively and RP-HPLC method has been developed using 1% methanolic HCl as diluent. Separations of drugs were achieved on L1 C18 100 A° column (250 x 4.6 mm, 5 μ) using 2 gm potassium dihydrogen phosphate and acetonitrile (70:30, v/v) as mobile phase with flow rate 1.0 mL/min. The detection wavelength was 290 nm. Validation of developed methods was done according to ICH Q2 (R1) guideline. Calibration curve was linear over the concentration range of 3-9 μg/mL (Fenbendazole) and 10-30 μg/mL (Niclosamide) for spectrophotometric method and 24-39 μg/mL (Fenbendazole) and 80-130 μg/mL (Niclosamide) for RP-HPLC method. The developed RP-HPLC and derivative spectrophotometric method were successfully applied for the quantitative determination of cited drugs in pharmaceutical dosage form. The correlation coefficients (r²) value greater than 0.995. Accuracy of methods were determined by recovery studies and it was found to be 98 to 102 %. The % RSD values for all the validation parameters were less than 2.0 % for both the methods. The developed UV and RP-HPLC methods were compared by t - test and it was found that t stat value was less than t critical, value for all. Hence there was no significant difference between the developed methods.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.61 n.2 20162016-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072016000200030en10.4067/S0717-97072016000200030
institution Scielo Chile
collection Scielo Chile
language English
topic Fenbendazole
Niclosamide
UV- Spectrophotometry
RP-HPLC
Validation
spellingShingle Fenbendazole
Niclosamide
UV- Spectrophotometry
RP-HPLC
Validation
SHAH,UMANG
GANDHI,AYUSHI
DAWAWALA,TRUPTI
REVERSE PHASE HPLC AND DERIVATIVE SPECTROPHOTOMETRIC METHODS FOR SIMULTANEOUS ESTIMATION OF FENBENDAZOLE AND NICLOSAMIDE IN PHARMACEUTICAL DOSAGE FORM
description The present study narrates the developed and validated simple, reliable, sensitive, precise and accurate Spectrophotometric and RP-HPLC methods for the simultaneous estimation of Fenbendazole and Niclosamide in pharmaceutical dosage form. In the first order derivative method 0.1 N methanolic HCl was used as diluent. The zero crossing point wavelengths selected for the analysis were 226 nm and 317 nm for Fenbendazole and Niclosamide, respectively and RP-HPLC method has been developed using 1% methanolic HCl as diluent. Separations of drugs were achieved on L1 C18 100 A° column (250 x 4.6 mm, 5 μ) using 2 gm potassium dihydrogen phosphate and acetonitrile (70:30, v/v) as mobile phase with flow rate 1.0 mL/min. The detection wavelength was 290 nm. Validation of developed methods was done according to ICH Q2 (R1) guideline. Calibration curve was linear over the concentration range of 3-9 μg/mL (Fenbendazole) and 10-30 μg/mL (Niclosamide) for spectrophotometric method and 24-39 μg/mL (Fenbendazole) and 80-130 μg/mL (Niclosamide) for RP-HPLC method. The developed RP-HPLC and derivative spectrophotometric method were successfully applied for the quantitative determination of cited drugs in pharmaceutical dosage form. The correlation coefficients (r²) value greater than 0.995. Accuracy of methods were determined by recovery studies and it was found to be 98 to 102 %. The % RSD values for all the validation parameters were less than 2.0 % for both the methods. The developed UV and RP-HPLC methods were compared by t - test and it was found that t stat value was less than t critical, value for all. Hence there was no significant difference between the developed methods.
author SHAH,UMANG
GANDHI,AYUSHI
DAWAWALA,TRUPTI
author_facet SHAH,UMANG
GANDHI,AYUSHI
DAWAWALA,TRUPTI
author_sort SHAH,UMANG
title REVERSE PHASE HPLC AND DERIVATIVE SPECTROPHOTOMETRIC METHODS FOR SIMULTANEOUS ESTIMATION OF FENBENDAZOLE AND NICLOSAMIDE IN PHARMACEUTICAL DOSAGE FORM
title_short REVERSE PHASE HPLC AND DERIVATIVE SPECTROPHOTOMETRIC METHODS FOR SIMULTANEOUS ESTIMATION OF FENBENDAZOLE AND NICLOSAMIDE IN PHARMACEUTICAL DOSAGE FORM
title_full REVERSE PHASE HPLC AND DERIVATIVE SPECTROPHOTOMETRIC METHODS FOR SIMULTANEOUS ESTIMATION OF FENBENDAZOLE AND NICLOSAMIDE IN PHARMACEUTICAL DOSAGE FORM
title_fullStr REVERSE PHASE HPLC AND DERIVATIVE SPECTROPHOTOMETRIC METHODS FOR SIMULTANEOUS ESTIMATION OF FENBENDAZOLE AND NICLOSAMIDE IN PHARMACEUTICAL DOSAGE FORM
title_full_unstemmed REVERSE PHASE HPLC AND DERIVATIVE SPECTROPHOTOMETRIC METHODS FOR SIMULTANEOUS ESTIMATION OF FENBENDAZOLE AND NICLOSAMIDE IN PHARMACEUTICAL DOSAGE FORM
title_sort reverse phase hplc and derivative spectrophotometric methods for simultaneous estimation of fenbendazole and niclosamide in pharmaceutical dosage form
publisher Sociedad Chilena de Química
publishDate 2016
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072016000200030
work_keys_str_mv AT shahumang reversephasehplcandderivativespectrophotometricmethodsforsimultaneousestimationoffenbendazoleandniclosamideinpharmaceuticaldosageform
AT gandhiayushi reversephasehplcandderivativespectrophotometricmethodsforsimultaneousestimationoffenbendazoleandniclosamideinpharmaceuticaldosageform
AT dawawalatrupti reversephasehplcandderivativespectrophotometricmethodsforsimultaneousestimationoffenbendazoleandniclosamideinpharmaceuticaldosageform
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