TEMPERATURE DEPENDENCE OF BILAYER PROPERTIES IN LIPOSOMES AND THE USE OF FLUORESCENT PROBES AS A TOOL TO ELUCIDATE THE PERMEATION MECHANISM OF HYDROPHILIC SOLUTES

Solute transport across lipidic membranes is a fundamental process for both living organisms and drug delivery. In order to establish the mechanism of solute passive transport through lipidic membranes, we determined the effect of temperature upon the rate of glucose transport (as hydrophilic solute...

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Autores principales:	AHUMADA,M, CALDERÓN,C, LISSIE,A
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2016
Materias:	liposome permeation glucose glucose oxidase fluorescent probe
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072016000300009
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spelling	oai:scielo:S0717-970720160003000092017-06-14TEMPERATURE DEPENDENCE OF BILAYER PROPERTIES IN LIPOSOMES AND THE USE OF FLUORESCENT PROBES AS A TOOL TO ELUCIDATE THE PERMEATION MECHANISM OF HYDROPHILIC SOLUTESAHUMADA,MCALDERÓN,CLISSIE,A liposome permeation glucose glucose oxidase fluorescent probe Solute transport across lipidic membranes is a fundamental process for both living organisms and drug delivery. In order to establish the mechanism of solute passive transport through lipidic membranes, we determined the effect of temperature upon the rate of glucose transport (as hydrophilic solute model) through dipalmitoylphosphatidylcholine large unilamellar liposomes and compare the results to those obtained for a variety of fluorescent probes (pyrene, PRODAN, diphenylhexatriene, diphenylhexatriene-TMA). All these probes, independent of their localization in the liposomes, report a monotonous change in the micro-properties sensed with temperature, with a maximum rate of change at the main transition temperature of the bilayer. These results contrast with those obtained for the rate of glucose influx, were it is observed a clear maximum rate of intake at the transition temperature. These contrasting results imply that the microviscosity (common factor for used probes) is not the property of the bilayer that controls the rate off solute transport which, at least in the vicinity of the main transition temperature, is regulated by formation of transient pores, particularly at the transition temperature (Tm). This would indicate that, at least near Tm, glucose and other hydrophilic solutes diffuses through transient pores formed in the bilayer. Interestingly, the presence of these pores does not affect the fluorescence characteristics of the probes.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.61 n.3 20162016-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072016000300009en10.4067/S0717-97072016000300009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	liposome permeation glucose glucose oxidase fluorescent probe
spellingShingle	liposome permeation glucose glucose oxidase fluorescent probe AHUMADA,M CALDERÓN,C LISSIE,A TEMPERATURE DEPENDENCE OF BILAYER PROPERTIES IN LIPOSOMES AND THE USE OF FLUORESCENT PROBES AS A TOOL TO ELUCIDATE THE PERMEATION MECHANISM OF HYDROPHILIC SOLUTES
description	Solute transport across lipidic membranes is a fundamental process for both living organisms and drug delivery. In order to establish the mechanism of solute passive transport through lipidic membranes, we determined the effect of temperature upon the rate of glucose transport (as hydrophilic solute model) through dipalmitoylphosphatidylcholine large unilamellar liposomes and compare the results to those obtained for a variety of fluorescent probes (pyrene, PRODAN, diphenylhexatriene, diphenylhexatriene-TMA). All these probes, independent of their localization in the liposomes, report a monotonous change in the micro-properties sensed with temperature, with a maximum rate of change at the main transition temperature of the bilayer. These results contrast with those obtained for the rate of glucose influx, were it is observed a clear maximum rate of intake at the transition temperature. These contrasting results imply that the microviscosity (common factor for used probes) is not the property of the bilayer that controls the rate off solute transport which, at least in the vicinity of the main transition temperature, is regulated by formation of transient pores, particularly at the transition temperature (Tm). This would indicate that, at least near Tm, glucose and other hydrophilic solutes diffuses through transient pores formed in the bilayer. Interestingly, the presence of these pores does not affect the fluorescence characteristics of the probes.
author	AHUMADA,M CALDERÓN,C LISSIE,A
author_facet	AHUMADA,M CALDERÓN,C LISSIE,A
author_sort	AHUMADA,M
title	TEMPERATURE DEPENDENCE OF BILAYER PROPERTIES IN LIPOSOMES AND THE USE OF FLUORESCENT PROBES AS A TOOL TO ELUCIDATE THE PERMEATION MECHANISM OF HYDROPHILIC SOLUTES
title_short	TEMPERATURE DEPENDENCE OF BILAYER PROPERTIES IN LIPOSOMES AND THE USE OF FLUORESCENT PROBES AS A TOOL TO ELUCIDATE THE PERMEATION MECHANISM OF HYDROPHILIC SOLUTES
title_full	TEMPERATURE DEPENDENCE OF BILAYER PROPERTIES IN LIPOSOMES AND THE USE OF FLUORESCENT PROBES AS A TOOL TO ELUCIDATE THE PERMEATION MECHANISM OF HYDROPHILIC SOLUTES
title_fullStr	TEMPERATURE DEPENDENCE OF BILAYER PROPERTIES IN LIPOSOMES AND THE USE OF FLUORESCENT PROBES AS A TOOL TO ELUCIDATE THE PERMEATION MECHANISM OF HYDROPHILIC SOLUTES
title_full_unstemmed	TEMPERATURE DEPENDENCE OF BILAYER PROPERTIES IN LIPOSOMES AND THE USE OF FLUORESCENT PROBES AS A TOOL TO ELUCIDATE THE PERMEATION MECHANISM OF HYDROPHILIC SOLUTES
title_sort	temperature dependence of bilayer properties in liposomes and the use of fluorescent probes as a tool to elucidate the permeation mechanism of hydrophilic solutes
publisher	Sociedad Chilena de Química
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072016000300009
work_keys_str_mv	AT ahumadam temperaturedependenceofbilayerpropertiesinliposomesandtheuseoffluorescentprobesasatooltoelucidatethepermeationmechanismofhydrophilicsolutes AT calderonc temperaturedependenceofbilayerpropertiesinliposomesandtheuseoffluorescentprobesasatooltoelucidatethepermeationmechanismofhydrophilicsolutes AT lissiea temperaturedependenceofbilayerpropertiesinliposomesandtheuseoffluorescentprobesasatooltoelucidatethepermeationmechanismofhydrophilicsolutes
_version_	1714200896089358336

TEMPERATURE DEPENDENCE OF BILAYER PROPERTIES IN LIPOSOMES AND THE USE OF FLUORESCENT PROBES AS A TOOL TO ELUCIDATE THE PERMEATION MECHANISM OF HYDROPHILIC SOLUTES

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