SOLID PHASE EXTRACTION AND LC-MS/MS METHOD FOR QUANTIFICATION OF VENLAFAXINE AND ITS ACTIVE METABOLITE O-DESMETHYL VENLAFAXINE IN RAT PLASMA

A rapid, simple, sensitive LC-MS/MS method involving a least pretreatment process has been proposed for the quantitative assessment of venlafaxine (VEN) and O-desmethyl venlafaxine (ODV) using cetirizine as an internal standard. The method was validated over the range of 1.03 ng/mL to 453.50 ng/mL (...

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Autores principales:	AHMAD,SARFARAZ, SIDDIQUI,MASOOM R, ALI,MD. S, WABAIDUR,SAIKH M, ALAM,MD. S, ALAM,NAWAZISH, ALOTHMAN,ZEID A, KHAN,MOONIS ALI, KHAN,MOHAMMAD R
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2016
Materias:	venlafaxine o-desmethyl venlafaxine pharmacokinetics solid phase extraction LC-MS/MS
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072016000300025
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spelling	oai:scielo:S0717-970720160003000252017-06-14SOLID PHASE EXTRACTION AND LC-MS/MS METHOD FOR QUANTIFICATION OF VENLAFAXINE AND ITS ACTIVE METABOLITE O-DESMETHYL VENLAFAXINE IN RAT PLASMAAHMAD,SARFARAZSIDDIQUI,MASOOM RALI,MD. SWABAIDUR,SAIKH MALAM,MD. SALAM,NAWAZISHALOTHMAN,ZEID AKHAN,MOONIS ALIKHAN,MOHAMMAD R venlafaxine o-desmethyl venlafaxine pharmacokinetics solid phase extraction LC-MS/MS A rapid, simple, sensitive LC-MS/MS method involving a least pretreatment process has been proposed for the quantitative assessment of venlafaxine (VEN) and O-desmethyl venlafaxine (ODV) using cetirizine as an internal standard. The method was validated over the range of 1.03 ng/mL to 453.50 ng/mL (venlafaxine) and 1.32 ng/mL to 585.21 ng/mL (O-desmethyl venlafaxine). The lowest limit of quantification for venlafaxine and O-desmethyl venlafaxine was found to be 1.03 ng/mL and 1.32 ng/mL, respectively. The solid phase extraction procedure provided reliable and reproducible recoveries of the drug as well as its active metabolite with no interference at their retention time. The recovery for all analyzed drugs was found to be in the range of 72.55% to 74.75%. The result indicates that the developed procedure could be considered suitable for carry out simultaneous preclinical pharmacokinetics studies for VEN and ODV.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.61 n.3 20162016-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072016000300025en10.4067/S0717-97072016000300025
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	venlafaxine o-desmethyl venlafaxine pharmacokinetics solid phase extraction LC-MS/MS
spellingShingle	venlafaxine o-desmethyl venlafaxine pharmacokinetics solid phase extraction LC-MS/MS AHMAD,SARFARAZ SIDDIQUI,MASOOM R ALI,MD. S WABAIDUR,SAIKH M ALAM,MD. S ALAM,NAWAZISH ALOTHMAN,ZEID A KHAN,MOONIS ALI KHAN,MOHAMMAD R SOLID PHASE EXTRACTION AND LC-MS/MS METHOD FOR QUANTIFICATION OF VENLAFAXINE AND ITS ACTIVE METABOLITE O-DESMETHYL VENLAFAXINE IN RAT PLASMA
description	A rapid, simple, sensitive LC-MS/MS method involving a least pretreatment process has been proposed for the quantitative assessment of venlafaxine (VEN) and O-desmethyl venlafaxine (ODV) using cetirizine as an internal standard. The method was validated over the range of 1.03 ng/mL to 453.50 ng/mL (venlafaxine) and 1.32 ng/mL to 585.21 ng/mL (O-desmethyl venlafaxine). The lowest limit of quantification for venlafaxine and O-desmethyl venlafaxine was found to be 1.03 ng/mL and 1.32 ng/mL, respectively. The solid phase extraction procedure provided reliable and reproducible recoveries of the drug as well as its active metabolite with no interference at their retention time. The recovery for all analyzed drugs was found to be in the range of 72.55% to 74.75%. The result indicates that the developed procedure could be considered suitable for carry out simultaneous preclinical pharmacokinetics studies for VEN and ODV.
author	AHMAD,SARFARAZ SIDDIQUI,MASOOM R ALI,MD. S WABAIDUR,SAIKH M ALAM,MD. S ALAM,NAWAZISH ALOTHMAN,ZEID A KHAN,MOONIS ALI KHAN,MOHAMMAD R
author_facet	AHMAD,SARFARAZ SIDDIQUI,MASOOM R ALI,MD. S WABAIDUR,SAIKH M ALAM,MD. S ALAM,NAWAZISH ALOTHMAN,ZEID A KHAN,MOONIS ALI KHAN,MOHAMMAD R
author_sort	AHMAD,SARFARAZ
title	SOLID PHASE EXTRACTION AND LC-MS/MS METHOD FOR QUANTIFICATION OF VENLAFAXINE AND ITS ACTIVE METABOLITE O-DESMETHYL VENLAFAXINE IN RAT PLASMA
title_short	SOLID PHASE EXTRACTION AND LC-MS/MS METHOD FOR QUANTIFICATION OF VENLAFAXINE AND ITS ACTIVE METABOLITE O-DESMETHYL VENLAFAXINE IN RAT PLASMA
title_full	SOLID PHASE EXTRACTION AND LC-MS/MS METHOD FOR QUANTIFICATION OF VENLAFAXINE AND ITS ACTIVE METABOLITE O-DESMETHYL VENLAFAXINE IN RAT PLASMA
title_fullStr	SOLID PHASE EXTRACTION AND LC-MS/MS METHOD FOR QUANTIFICATION OF VENLAFAXINE AND ITS ACTIVE METABOLITE O-DESMETHYL VENLAFAXINE IN RAT PLASMA
title_full_unstemmed	SOLID PHASE EXTRACTION AND LC-MS/MS METHOD FOR QUANTIFICATION OF VENLAFAXINE AND ITS ACTIVE METABOLITE O-DESMETHYL VENLAFAXINE IN RAT PLASMA
title_sort	solid phase extraction and lc-ms/ms method for quantification of venlafaxine and its active metabolite o-desmethyl venlafaxine in rat plasma
publisher	Sociedad Chilena de Química
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072016000300025
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SOLID PHASE EXTRACTION AND LC-MS/MS METHOD FOR QUANTIFICATION OF VENLAFAXINE AND ITS ACTIVE METABOLITE O-DESMETHYL VENLAFAXINE IN RAT PLASMA

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