SIMULTANEOUS DETERMINATION OF DIFFERENT FLAVONOIDS IN HUMAN PLASMA BY A SIMPLE HPLC ASSAY

A simple HPLC method for simultaneous determination of five flavonoids in human plasma was developed. Following addition of catechin as internal standard (IS), luteolin-7-glucoside, rutin, apigenin-7-glucoside, quercetin and baicalein were isolated from human plasma by liquid-liquid extraction with...

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Autores principales:	MÜLLER-SEPÚLVEDA,A, LETELIER,M. E., SAN MARTIN,B, SAAVEDRA-SAAVEDRA,I
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2016
Materias:	Flavonoids Catechin Rutin Apigenin-7-glucoside Luteolin-7-glucoside Baicalein Quercetin HPLC
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072016000400003
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spelling	oai:scielo:S0717-970720160004000032017-01-16SIMULTANEOUS DETERMINATION OF DIFFERENT FLAVONOIDS IN HUMAN PLASMA BY A SIMPLE HPLC ASSAYMÜLLER-SEPÚLVEDA,ALETELIER,M. E.SAN MARTIN,BSAAVEDRA-SAAVEDRA,I Flavonoids Catechin Rutin Apigenin-7-glucoside Luteolin-7-glucoside Baicalein Quercetin HPLC A simple HPLC method for simultaneous determination of five flavonoids in human plasma was developed. Following addition of catechin as internal standard (IS), luteolin-7-glucoside, rutin, apigenin-7-glucoside, quercetin and baicalein were isolated from human plasma by liquid-liquid extraction with acetone. The chromatographic separation was achieved in a DAD-HPLC equipment on a reversed-phase C18 column using a gradient mobile phase consisting of 0.1% acetic acid + methanol: acetonitrile: acetic acid (90:10:1, v/v) and running at a flow rate of 1 mL/min. The effluent was monitored at a wavelength of 280 ƞm, as well as IS was well separated from each other and free from interference from blank plasma and other components in plasma. The calibration curve was constructed by plotting peak area ratio of analytes to IS vs. concentration. The method showed good linearity over range of 5 - 30 μg/mL for the five standards used (r>0.996). The developed method may be used not only to identify the analytes assayed but, simultaneously measure plasma concentrations of these herbal compounds.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.61 n.4 20162016-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072016000400003en10.4067/S0717-97072016000400003
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Flavonoids Catechin Rutin Apigenin-7-glucoside Luteolin-7-glucoside Baicalein Quercetin HPLC
spellingShingle	Flavonoids Catechin Rutin Apigenin-7-glucoside Luteolin-7-glucoside Baicalein Quercetin HPLC MÜLLER-SEPÚLVEDA,A LETELIER,M. E. SAN MARTIN,B SAAVEDRA-SAAVEDRA,I SIMULTANEOUS DETERMINATION OF DIFFERENT FLAVONOIDS IN HUMAN PLASMA BY A SIMPLE HPLC ASSAY
description	A simple HPLC method for simultaneous determination of five flavonoids in human plasma was developed. Following addition of catechin as internal standard (IS), luteolin-7-glucoside, rutin, apigenin-7-glucoside, quercetin and baicalein were isolated from human plasma by liquid-liquid extraction with acetone. The chromatographic separation was achieved in a DAD-HPLC equipment on a reversed-phase C18 column using a gradient mobile phase consisting of 0.1% acetic acid + methanol: acetonitrile: acetic acid (90:10:1, v/v) and running at a flow rate of 1 mL/min. The effluent was monitored at a wavelength of 280 ƞm, as well as IS was well separated from each other and free from interference from blank plasma and other components in plasma. The calibration curve was constructed by plotting peak area ratio of analytes to IS vs. concentration. The method showed good linearity over range of 5 - 30 μg/mL for the five standards used (r>0.996). The developed method may be used not only to identify the analytes assayed but, simultaneously measure plasma concentrations of these herbal compounds.
author	MÜLLER-SEPÚLVEDA,A LETELIER,M. E. SAN MARTIN,B SAAVEDRA-SAAVEDRA,I
author_facet	MÜLLER-SEPÚLVEDA,A LETELIER,M. E. SAN MARTIN,B SAAVEDRA-SAAVEDRA,I
author_sort	MÜLLER-SEPÚLVEDA,A
title	SIMULTANEOUS DETERMINATION OF DIFFERENT FLAVONOIDS IN HUMAN PLASMA BY A SIMPLE HPLC ASSAY
title_short	SIMULTANEOUS DETERMINATION OF DIFFERENT FLAVONOIDS IN HUMAN PLASMA BY A SIMPLE HPLC ASSAY
title_full	SIMULTANEOUS DETERMINATION OF DIFFERENT FLAVONOIDS IN HUMAN PLASMA BY A SIMPLE HPLC ASSAY
title_fullStr	SIMULTANEOUS DETERMINATION OF DIFFERENT FLAVONOIDS IN HUMAN PLASMA BY A SIMPLE HPLC ASSAY
title_full_unstemmed	SIMULTANEOUS DETERMINATION OF DIFFERENT FLAVONOIDS IN HUMAN PLASMA BY A SIMPLE HPLC ASSAY
title_sort	simultaneous determination of different flavonoids in human plasma by a simple hplc assay
publisher	Sociedad Chilena de Química
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072016000400003
work_keys_str_mv	AT mullersepulvedaa simultaneousdeterminationofdifferentflavonoidsinhumanplasmabyasimplehplcassay AT letelierme simultaneousdeterminationofdifferentflavonoidsinhumanplasmabyasimplehplcassay AT sanmartinb simultaneousdeterminationofdifferentflavonoidsinhumanplasmabyasimplehplcassay AT saavedrasaavedrai simultaneousdeterminationofdifferentflavonoidsinhumanplasmabyasimplehplcassay
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SIMULTANEOUS DETERMINATION OF DIFFERENT FLAVONOIDS IN HUMAN PLASMA BY A SIMPLE HPLC ASSAY

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