EFFECT OF GAMMA-IRRADIATION ON BIODEGRADABLE MICROSPHERES LOADED WITH RASAGILINE MESYLATE

In the present study, the influence of gamma-irradiation was evaluated on the physicochemical characteristics and in vitro release of rasagiline mesylate (RM), a selective MAO-B inhibitor used in Parkinson’s disease, from poly(D,L-lactide-co-glycolide) (PLGA) microspheres. Microspheres wer...

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Autores principales:	FERNÁNDEZ,MARCOS, BARCIA,EMILIA, NEGRO,SOFÍA
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2016
Materias:	Gamma-irradiation Microspheres PLGA Rasagiline mesylate Parkinson’s disease
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072016000400005
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spelling	oai:scielo:S0717-970720160004000052017-01-16EFFECT OF GAMMA-IRRADIATION ON BIODEGRADABLE MICROSPHERES LOADED WITH RASAGILINE MESYLATEFERNÁNDEZ,MARCOSBARCIA,EMILIANEGRO,SOFÍA Gamma-irradiation Microspheres PLGA Rasagiline mesylate Parkinson’s disease In the present study, the influence of gamma-irradiation was evaluated on the physicochemical characteristics and in vitro release of rasagiline mesylate (RM), a selective MAO-B inhibitor used in Parkinson’s disease, from poly(D,L-lactide-co-glycolide) (PLGA) microspheres. Microspheres were prepared using PLGA 50:50 by the solvent evaporation technique (O/W emulsion). Microspheres were sterilized by gamma-irradiation and their influence was assessed by scanning electron microscopy (SEM), laser light diffraction, differential scanning calorimetry (DSC), X-ray diffraction (XRD), gel permeation chromatography (GPC), encapsulation efficiency (EE) and in vitro drug release. Gamma-irradiation of RM-loaded microspheres did not affect EE, DSC and XRD patterns. After gamma-irradiation, changes on the surface were observed by SEM, but no significant difference in mean particle size was observed. GPC measurements showed a decrease in molecular weight of the polymer after five days of in vitro release. The similarity factor value between irradiated and non-irradiates microspheres was <50, indicating the non-similarity of the release profiles. The sterilization technique had an effect on the integrity of polymeric system, significantly affecting in vitro release of RM from PLGA microspheres. Therefore, from our results we conclude that gamma-irradiation is not a suitable sterilization procedure for this formulation.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.61 n.4 20162016-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072016000400005en10.4067/S0717-97072016000400005
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Gamma-irradiation Microspheres PLGA Rasagiline mesylate Parkinson’s disease
spellingShingle	Gamma-irradiation Microspheres PLGA Rasagiline mesylate Parkinson’s disease FERNÁNDEZ,MARCOS BARCIA,EMILIA NEGRO,SOFÍA EFFECT OF GAMMA-IRRADIATION ON BIODEGRADABLE MICROSPHERES LOADED WITH RASAGILINE MESYLATE
description	In the present study, the influence of gamma-irradiation was evaluated on the physicochemical characteristics and in vitro release of rasagiline mesylate (RM), a selective MAO-B inhibitor used in Parkinson’s disease, from poly(D,L-lactide-co-glycolide) (PLGA) microspheres. Microspheres were prepared using PLGA 50:50 by the solvent evaporation technique (O/W emulsion). Microspheres were sterilized by gamma-irradiation and their influence was assessed by scanning electron microscopy (SEM), laser light diffraction, differential scanning calorimetry (DSC), X-ray diffraction (XRD), gel permeation chromatography (GPC), encapsulation efficiency (EE) and in vitro drug release. Gamma-irradiation of RM-loaded microspheres did not affect EE, DSC and XRD patterns. After gamma-irradiation, changes on the surface were observed by SEM, but no significant difference in mean particle size was observed. GPC measurements showed a decrease in molecular weight of the polymer after five days of in vitro release. The similarity factor value between irradiated and non-irradiates microspheres was <50, indicating the non-similarity of the release profiles. The sterilization technique had an effect on the integrity of polymeric system, significantly affecting in vitro release of RM from PLGA microspheres. Therefore, from our results we conclude that gamma-irradiation is not a suitable sterilization procedure for this formulation.
author	FERNÁNDEZ,MARCOS BARCIA,EMILIA NEGRO,SOFÍA
author_facet	FERNÁNDEZ,MARCOS BARCIA,EMILIA NEGRO,SOFÍA
author_sort	FERNÁNDEZ,MARCOS
title	EFFECT OF GAMMA-IRRADIATION ON BIODEGRADABLE MICROSPHERES LOADED WITH RASAGILINE MESYLATE
title_short	EFFECT OF GAMMA-IRRADIATION ON BIODEGRADABLE MICROSPHERES LOADED WITH RASAGILINE MESYLATE
title_full	EFFECT OF GAMMA-IRRADIATION ON BIODEGRADABLE MICROSPHERES LOADED WITH RASAGILINE MESYLATE
title_fullStr	EFFECT OF GAMMA-IRRADIATION ON BIODEGRADABLE MICROSPHERES LOADED WITH RASAGILINE MESYLATE
title_full_unstemmed	EFFECT OF GAMMA-IRRADIATION ON BIODEGRADABLE MICROSPHERES LOADED WITH RASAGILINE MESYLATE
title_sort	effect of gamma-irradiation on biodegradable microspheres loaded with rasagiline mesylate
publisher	Sociedad Chilena de Química
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072016000400005
work_keys_str_mv	AT fernandezmarcos effectofgammairradiationonbiodegradablemicrospheresloadedwithrasagilinemesylate AT barciaemilia effectofgammairradiationonbiodegradablemicrospheresloadedwithrasagilinemesylate AT negrosofia effectofgammairradiationonbiodegradablemicrospheresloadedwithrasagilinemesylate
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EFFECT OF GAMMA-IRRADIATION ON BIODEGRADABLE MICROSPHERES LOADED WITH RASAGILINE MESYLATE

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