CONFIRMATION OF DEOXYNIV ALENOL PRESENCE IN CHILEAN WHEAT BY HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY-MASS SPECTROMETRY

Deoxynivalenol (DON) is a mycotoxin that belongs to trichothecene subgroup B. Due its detrimental effects for human health, DON has been analyzed and detected by nearly all chromatographic methods. The present work reports for the first time the application of HPTLC/MS to confirm the presence of DON...

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Autores principales:	VEGA-HERRERA,MARIO, MADARIAGA,RICARDO, ARANDA,MARIO, MORLOCK,GERTRUD E
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2017
Materias:	Deoxynivalenol mycotoxins HPTLC/MS Fusarium TLC
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072017000200003
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spelling	oai:scielo:S0717-970720170002000032017-06-30CONFIRMATION OF DEOXYNIV ALENOL PRESENCE IN CHILEAN WHEAT BY HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY-MASS SPECTROMETRYVEGA-HERRERA,MARIOMADARIAGA,RICARDOARANDA,MARIOMORLOCK,GERTRUD E Deoxynivalenol mycotoxins HPTLC/MS Fusarium TLC Deoxynivalenol (DON) is a mycotoxin that belongs to trichothecene subgroup B. Due its detrimental effects for human health, DON has been analyzed and detected by nearly all chromatographic methods. The present work reports for the first time the application of HPTLC/MS to confirm the presence of DON in wheat crops. Chromatography was performed on silica gel 60 F254 HPTLC plates using toluene - ethyl acetate - formic acid 6:3:1 (V/V/V) as mobile phase. After post-chromatographic derivatization with 10 % AlCl3, fluorescence detection was carried out at UV 366/>400 nm. Bands identity and purity was confirmed by mass spectrometry acquiring analytes directly from the sample bands by means of TLC/MS elution head-based interface. Both, sample and standard bands showed clear mass signals at m/z 341 that corresponds to the formate adduct of DON [M+HCOO]-. Thus, the presence of DON in the Chilean wheat sample was confirmed.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.62 n.2 20172017-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072017000200003en10.4067/S0717-97072017000200003
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Deoxynivalenol mycotoxins HPTLC/MS Fusarium TLC
spellingShingle	Deoxynivalenol mycotoxins HPTLC/MS Fusarium TLC VEGA-HERRERA,MARIO MADARIAGA,RICARDO ARANDA,MARIO MORLOCK,GERTRUD E CONFIRMATION OF DEOXYNIV ALENOL PRESENCE IN CHILEAN WHEAT BY HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY-MASS SPECTROMETRY
description	Deoxynivalenol (DON) is a mycotoxin that belongs to trichothecene subgroup B. Due its detrimental effects for human health, DON has been analyzed and detected by nearly all chromatographic methods. The present work reports for the first time the application of HPTLC/MS to confirm the presence of DON in wheat crops. Chromatography was performed on silica gel 60 F254 HPTLC plates using toluene - ethyl acetate - formic acid 6:3:1 (V/V/V) as mobile phase. After post-chromatographic derivatization with 10 % AlCl3, fluorescence detection was carried out at UV 366/>400 nm. Bands identity and purity was confirmed by mass spectrometry acquiring analytes directly from the sample bands by means of TLC/MS elution head-based interface. Both, sample and standard bands showed clear mass signals at m/z 341 that corresponds to the formate adduct of DON [M+HCOO]-. Thus, the presence of DON in the Chilean wheat sample was confirmed.
author	VEGA-HERRERA,MARIO MADARIAGA,RICARDO ARANDA,MARIO MORLOCK,GERTRUD E
author_facet	VEGA-HERRERA,MARIO MADARIAGA,RICARDO ARANDA,MARIO MORLOCK,GERTRUD E
author_sort	VEGA-HERRERA,MARIO
title	CONFIRMATION OF DEOXYNIV ALENOL PRESENCE IN CHILEAN WHEAT BY HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY-MASS SPECTROMETRY
title_short	CONFIRMATION OF DEOXYNIV ALENOL PRESENCE IN CHILEAN WHEAT BY HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY-MASS SPECTROMETRY
title_full	CONFIRMATION OF DEOXYNIV ALENOL PRESENCE IN CHILEAN WHEAT BY HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY-MASS SPECTROMETRY
title_fullStr	CONFIRMATION OF DEOXYNIV ALENOL PRESENCE IN CHILEAN WHEAT BY HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY-MASS SPECTROMETRY
title_full_unstemmed	CONFIRMATION OF DEOXYNIV ALENOL PRESENCE IN CHILEAN WHEAT BY HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY-MASS SPECTROMETRY
title_sort	confirmation of deoxyniv alenol presence in chilean wheat by high-performance thin-layer chromatography-mass spectrometry
publisher	Sociedad Chilena de Química
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072017000200003
work_keys_str_mv	AT vegaherreramario confirmationofdeoxynivalenolpresenceinchileanwheatbyhighperformancethinlayerchromatographymassspectrometry AT madariagaricardo confirmationofdeoxynivalenolpresenceinchileanwheatbyhighperformancethinlayerchromatographymassspectrometry AT arandamario confirmationofdeoxynivalenolpresenceinchileanwheatbyhighperformancethinlayerchromatographymassspectrometry AT morlockgertrude confirmationofdeoxynivalenolpresenceinchileanwheatbyhighperformancethinlayerchromatographymassspectrometry
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CONFIRMATION OF DEOXYNIV ALENOL PRESENCE IN CHILEAN WHEAT BY HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY-MASS SPECTROMETRY

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