MAGNETIC SOLID PHASE EXTRACTION FOR A NEW HPLC METHOD FOR THE DETERMINATION OF GEMIFLOXACIN IN HUMAN PLASMA AND BREAST MILK

A simple analytical procedure, magnetic solid phase extraction combination to HPLC, has been developed for the analysis of gemifloxacin in human plasma and breast milk. Before chromatographic separation, magnetic solid phase extraction has been applied for sample preparation which is currently preff...

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Autores principales:	DURMUS,ZEHRA, TEKKELI,SERIFE EVRIM KEPEKCI, KIZILTAS,MUSTAFA VULKAN, ONAL,ARMAGAN
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2017
Materias:	HPLC Gemifloxacin Magnetic solid phase extraction Plasma Breast milk UV detection
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072017000200012
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spelling	oai:scielo:S0717-970720170002000122017-06-30MAGNETIC SOLID PHASE EXTRACTION FOR A NEW HPLC METHOD FOR THE DETERMINATION OF GEMIFLOXACIN IN HUMAN PLASMA AND BREAST MILKDURMUS,ZEHRATEKKELI,SERIFE EVRIM KEPEKCIKIZILTAS,MUSTAFA VULKANONAL,ARMAGAN HPLC Gemifloxacin Magnetic solid phase extraction Plasma Breast milk UV detection A simple analytical procedure, magnetic solid phase extraction combination to HPLC, has been developed for the analysis of gemifloxacin in human plasma and breast milk. Before chromatographic separation, magnetic solid phase extraction has been applied for sample preparation which is currently preffered extraction technique accordingly its simple, fast and efficient procedure. Fe3O4 magnetic nanoparticles have been used as magnetic adsorbents, the adsorption process has been optimized. RP C18 column has been used with mobile phase composed of acetonitrile-10 mM orthophosphoric acid including 1 mL/L triethylamine (60:40) by isocratic elution with flow rate of 1.3 mL/min. Calibration curve is linear over the range of 0.5-30 μg/mL and 0.5-20 μg/mL for plasma and breast milk, respectively. LOD and LOQ has been found to be 0.15 and 0.5 μg/mL for both matrices. Intraday and interday RSD values are less than 3.57% for both assays. Moreover, the newly developed method provides fast, simple, cost reduced and sensitive assay for gemifloxacin.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.62 n.2 20172017-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072017000200012en10.4067/S0717-97072017000200012
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	HPLC Gemifloxacin Magnetic solid phase extraction Plasma Breast milk UV detection
spellingShingle	HPLC Gemifloxacin Magnetic solid phase extraction Plasma Breast milk UV detection DURMUS,ZEHRA TEKKELI,SERIFE EVRIM KEPEKCI KIZILTAS,MUSTAFA VULKAN ONAL,ARMAGAN MAGNETIC SOLID PHASE EXTRACTION FOR A NEW HPLC METHOD FOR THE DETERMINATION OF GEMIFLOXACIN IN HUMAN PLASMA AND BREAST MILK
description	A simple analytical procedure, magnetic solid phase extraction combination to HPLC, has been developed for the analysis of gemifloxacin in human plasma and breast milk. Before chromatographic separation, magnetic solid phase extraction has been applied for sample preparation which is currently preffered extraction technique accordingly its simple, fast and efficient procedure. Fe3O4 magnetic nanoparticles have been used as magnetic adsorbents, the adsorption process has been optimized. RP C18 column has been used with mobile phase composed of acetonitrile-10 mM orthophosphoric acid including 1 mL/L triethylamine (60:40) by isocratic elution with flow rate of 1.3 mL/min. Calibration curve is linear over the range of 0.5-30 μg/mL and 0.5-20 μg/mL for plasma and breast milk, respectively. LOD and LOQ has been found to be 0.15 and 0.5 μg/mL for both matrices. Intraday and interday RSD values are less than 3.57% for both assays. Moreover, the newly developed method provides fast, simple, cost reduced and sensitive assay for gemifloxacin.
author	DURMUS,ZEHRA TEKKELI,SERIFE EVRIM KEPEKCI KIZILTAS,MUSTAFA VULKAN ONAL,ARMAGAN
author_facet	DURMUS,ZEHRA TEKKELI,SERIFE EVRIM KEPEKCI KIZILTAS,MUSTAFA VULKAN ONAL,ARMAGAN
author_sort	DURMUS,ZEHRA
title	MAGNETIC SOLID PHASE EXTRACTION FOR A NEW HPLC METHOD FOR THE DETERMINATION OF GEMIFLOXACIN IN HUMAN PLASMA AND BREAST MILK
title_short	MAGNETIC SOLID PHASE EXTRACTION FOR A NEW HPLC METHOD FOR THE DETERMINATION OF GEMIFLOXACIN IN HUMAN PLASMA AND BREAST MILK
title_full	MAGNETIC SOLID PHASE EXTRACTION FOR A NEW HPLC METHOD FOR THE DETERMINATION OF GEMIFLOXACIN IN HUMAN PLASMA AND BREAST MILK
title_fullStr	MAGNETIC SOLID PHASE EXTRACTION FOR A NEW HPLC METHOD FOR THE DETERMINATION OF GEMIFLOXACIN IN HUMAN PLASMA AND BREAST MILK
title_full_unstemmed	MAGNETIC SOLID PHASE EXTRACTION FOR A NEW HPLC METHOD FOR THE DETERMINATION OF GEMIFLOXACIN IN HUMAN PLASMA AND BREAST MILK
title_sort	magnetic solid phase extraction for a new hplc method for the determination of gemifloxacin in human plasma and breast milk
publisher	Sociedad Chilena de Química
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072017000200012
work_keys_str_mv	AT durmuszehra magneticsolidphaseextractionforanewhplcmethodforthedeterminationofgemifloxacininhumanplasmaandbreastmilk AT tekkeliserifeevrimkepekci magneticsolidphaseextractionforanewhplcmethodforthedeterminationofgemifloxacininhumanplasmaandbreastmilk AT kiziltasmustafavulkan magneticsolidphaseextractionforanewhplcmethodforthedeterminationofgemifloxacininhumanplasmaandbreastmilk AT onalarmagan magneticsolidphaseextractionforanewhplcmethodforthedeterminationofgemifloxacininhumanplasmaandbreastmilk
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MAGNETIC SOLID PHASE EXTRACTION FOR A NEW HPLC METHOD FOR THE DETERMINATION OF GEMIFLOXACIN IN HUMAN PLASMA AND BREAST MILK

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