STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF CIPROFLOXACIN AND DEXAMETHASONE IN BINARY COMBINATION

ABSTRACT A simple and isocratic HPLC method with stability indicating nature was developed and then subsequently validated for simultaneous determination of ciprofloxacin and dexamethasone in pharmaceutical formulations, human serum and urine. Best chromatographic separations were attained within ru...

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Autores principales:	RAZZAQ,SYED NAEEM, ASHFAQ,MUHAMMAD, MARIAM,IRFANA, KHAN,ISLAM ULLAH, RAZZAQ,SYED SALEEM, MUSTAFA,GHULAM
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2017
Materias:	Liquid Chromatographic Ciprofloxacin Dexamethasone Degradation Products ICH Guidelines
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072017000303572
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spelling	oai:scielo:S0717-970720170003035722017-09-08STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF CIPROFLOXACIN AND DEXAMETHASONE IN BINARY COMBINATIONRAZZAQ,SYED NAEEMASHFAQ,MUHAMMADMARIAM,IRFANAKHAN,ISLAM ULLAHRAZZAQ,SYED SALEEMMUSTAFA,GHULAM Liquid Chromatographic Ciprofloxacin Dexamethasone Degradation Products ICH Guidelines ABSTRACT A simple and isocratic HPLC method with stability indicating nature was developed and then subsequently validated for simultaneous determination of ciprofloxacin and dexamethasone in pharmaceutical formulations, human serum and urine. Best chromatographic separations were attained within run time of 10 minutes using C8 as stationary phase and mixture of phosphate buffer and methanol (41:59 v/v) as mobile phase. The mobile phase was flowed at 1.5 mL min−1 with detection of both the analytes at 270 nm using photodiode array detector. Validation of the method was accomplished using specificity, linearity, accuracy, precision, robustness, LOD and LOQ. The method was found linear from 3-21 mg mL−1 for ciprofloxacin (r2 ≥ 0.999) and 1-7 mg mL−1 for dexamethasone (r2 ≥ 0.999). The %age recoveries of ciprofloxacin in spiked human urine and serum were ≥99% and ≥85% respectively, while for dexamethasone they were ≥97% in both matrices. The method proficiently separated the peaks of ciprofloxacin and dexamethasone from all types of interfering substances including degradation products/impurities with purity index ≥ 0.9998. The method thus was stability-indicating and can be employed for simultaneous analysis of ciprofloxacin and dexamethasone in complex matrices involving multiple components in the mixture.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.62 n.3 20172017-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072017000303572en10.4067/s0717-97072017000303572
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Liquid Chromatographic Ciprofloxacin Dexamethasone Degradation Products ICH Guidelines
spellingShingle	Liquid Chromatographic Ciprofloxacin Dexamethasone Degradation Products ICH Guidelines RAZZAQ,SYED NAEEM ASHFAQ,MUHAMMAD MARIAM,IRFANA KHAN,ISLAM ULLAH RAZZAQ,SYED SALEEM MUSTAFA,GHULAM STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF CIPROFLOXACIN AND DEXAMETHASONE IN BINARY COMBINATION
description	ABSTRACT A simple and isocratic HPLC method with stability indicating nature was developed and then subsequently validated for simultaneous determination of ciprofloxacin and dexamethasone in pharmaceutical formulations, human serum and urine. Best chromatographic separations were attained within run time of 10 minutes using C8 as stationary phase and mixture of phosphate buffer and methanol (41:59 v/v) as mobile phase. The mobile phase was flowed at 1.5 mL min−1 with detection of both the analytes at 270 nm using photodiode array detector. Validation of the method was accomplished using specificity, linearity, accuracy, precision, robustness, LOD and LOQ. The method was found linear from 3-21 mg mL−1 for ciprofloxacin (r2 ≥ 0.999) and 1-7 mg mL−1 for dexamethasone (r2 ≥ 0.999). The %age recoveries of ciprofloxacin in spiked human urine and serum were ≥99% and ≥85% respectively, while for dexamethasone they were ≥97% in both matrices. The method proficiently separated the peaks of ciprofloxacin and dexamethasone from all types of interfering substances including degradation products/impurities with purity index ≥ 0.9998. The method thus was stability-indicating and can be employed for simultaneous analysis of ciprofloxacin and dexamethasone in complex matrices involving multiple components in the mixture.
author	RAZZAQ,SYED NAEEM ASHFAQ,MUHAMMAD MARIAM,IRFANA KHAN,ISLAM ULLAH RAZZAQ,SYED SALEEM MUSTAFA,GHULAM
author_facet	RAZZAQ,SYED NAEEM ASHFAQ,MUHAMMAD MARIAM,IRFANA KHAN,ISLAM ULLAH RAZZAQ,SYED SALEEM MUSTAFA,GHULAM
author_sort	RAZZAQ,SYED NAEEM
title	STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF CIPROFLOXACIN AND DEXAMETHASONE IN BINARY COMBINATION
title_short	STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF CIPROFLOXACIN AND DEXAMETHASONE IN BINARY COMBINATION
title_full	STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF CIPROFLOXACIN AND DEXAMETHASONE IN BINARY COMBINATION
title_fullStr	STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF CIPROFLOXACIN AND DEXAMETHASONE IN BINARY COMBINATION
title_full_unstemmed	STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF CIPROFLOXACIN AND DEXAMETHASONE IN BINARY COMBINATION
title_sort	stability indicating rp-hplc method for simultaneous determination of ciprofloxacin and dexamethasone in binary combination
publisher	Sociedad Chilena de Química
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072017000303572
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_version_	1714200913686560768

STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF CIPROFLOXACIN AND DEXAMETHASONE IN BINARY COMBINATION

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