ANTIBACTERIAL ACTIVITY AND HUMAN CELL CYTOTOXIC OF COBALT (III) COMPLEXES WITH 1,10-PHENANTHROLINE AND CARBOHYDRATE LIGANDS

ABSTRACT The mononuclear cobalt (III) complex derived from 1,10-phenanthroline with lactose [Co(phen)2lactose]Cl2·3H2O (1) has been prepared and its properties have been compared with the sucrose complex [Co(Phen)2sucrose]Cl2·3H2O(2) and the complex without carbohydrate [Co(Phen)2Cl2]Cl·3H2O(3). The...

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Autores principales:	PARADA,JOSÉ, ATRIA,ANA MARÍA, BAGGIO,RICARDO, WIESE,GUILLERMO, LAGOS,SEBASTIÁN, PAVÓN,ALEQUIS, RIVAS,ELIZABETH, NAVARRO,LAURA, CORSINI,GINO
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2017
Materias:	Phenanthroline Cobalt complexes Carbohydrate antibacterial cytotoxicity
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072017000403746
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spelling	oai:scielo:S0717-970720170004037462018-04-02ANTIBACTERIAL ACTIVITY AND HUMAN CELL CYTOTOXIC OF COBALT (III) COMPLEXES WITH 1,10-PHENANTHROLINE AND CARBOHYDRATE LIGANDSPARADA,JOSÉATRIA,ANA MARÍABAGGIO,RICARDOWIESE,GUILLERMOLAGOS,SEBASTIÁNPAVÓN,ALEQUISRIVAS,ELIZABETHNAVARRO,LAURACORSINI,GINO Phenanthroline Cobalt complexes Carbohydrate antibacterial cytotoxicity ABSTRACT The mononuclear cobalt (III) complex derived from 1,10-phenanthroline with lactose [Co(phen)2lactose]Cl2·3H2O (1) has been prepared and its properties have been compared with the sucrose complex [Co(Phen)2sucrose]Cl2·3H2O(2) and the complex without carbohydrate [Co(Phen)2Cl2]Cl·3H2O(3). The chemical structure of (1) was assigned by 1H-NMR, IR, CD and UV-Vis spectral data. The antibacterial activity of (1) - (3) was evaluated by disc-diffusion assays, using Gram-negative and positive bacteria. The minimum inhibitory concentration of the three complexes on the studied bacteria and their cytotoxicity on HEK293 human cells was determined. A colorimetric plate assay was used to distinguish bacteriostatic from bactericidal effect. Finally, the complexes uptake mechanism was evaluated using bacteria with mutated genes that encode for carbohydrate and siderophore receptors. The results indicate that complex (1) has an antibacterial activity similar to (3), while (2) presents a more restricted one. Moreover, all three complexes act by a bacteriostatic effect against bacterial cells and both (1) and (3) use a siderophore uptake mechanism to enter on bacterial cytoplasm. Cytotoxicity assays show that carbohydrate complexes are not cytotoxic to human cells, in contrast with complex (3), which is highly toxic. These results suggest that the use of the lactose ligand would maintain the antibacterial activity and uptake mechanism of the complex at reasonable levels, and would also reduce its toxicity against human cells. Thus, its strategic use would allow a decrease in toxicity of complexes used in eventual studies on eukaryotic systems.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.62 n.4 20172017-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072017000403746en10.4067/s0717-97072017000403746
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Phenanthroline Cobalt complexes Carbohydrate antibacterial cytotoxicity
spellingShingle	Phenanthroline Cobalt complexes Carbohydrate antibacterial cytotoxicity PARADA,JOSÉ ATRIA,ANA MARÍA BAGGIO,RICARDO WIESE,GUILLERMO LAGOS,SEBASTIÁN PAVÓN,ALEQUIS RIVAS,ELIZABETH NAVARRO,LAURA CORSINI,GINO ANTIBACTERIAL ACTIVITY AND HUMAN CELL CYTOTOXIC OF COBALT (III) COMPLEXES WITH 1,10-PHENANTHROLINE AND CARBOHYDRATE LIGANDS
description	ABSTRACT The mononuclear cobalt (III) complex derived from 1,10-phenanthroline with lactose [Co(phen)2lactose]Cl2·3H2O (1) has been prepared and its properties have been compared with the sucrose complex [Co(Phen)2sucrose]Cl2·3H2O(2) and the complex without carbohydrate [Co(Phen)2Cl2]Cl·3H2O(3). The chemical structure of (1) was assigned by 1H-NMR, IR, CD and UV-Vis spectral data. The antibacterial activity of (1) - (3) was evaluated by disc-diffusion assays, using Gram-negative and positive bacteria. The minimum inhibitory concentration of the three complexes on the studied bacteria and their cytotoxicity on HEK293 human cells was determined. A colorimetric plate assay was used to distinguish bacteriostatic from bactericidal effect. Finally, the complexes uptake mechanism was evaluated using bacteria with mutated genes that encode for carbohydrate and siderophore receptors. The results indicate that complex (1) has an antibacterial activity similar to (3), while (2) presents a more restricted one. Moreover, all three complexes act by a bacteriostatic effect against bacterial cells and both (1) and (3) use a siderophore uptake mechanism to enter on bacterial cytoplasm. Cytotoxicity assays show that carbohydrate complexes are not cytotoxic to human cells, in contrast with complex (3), which is highly toxic. These results suggest that the use of the lactose ligand would maintain the antibacterial activity and uptake mechanism of the complex at reasonable levels, and would also reduce its toxicity against human cells. Thus, its strategic use would allow a decrease in toxicity of complexes used in eventual studies on eukaryotic systems.
author	PARADA,JOSÉ ATRIA,ANA MARÍA BAGGIO,RICARDO WIESE,GUILLERMO LAGOS,SEBASTIÁN PAVÓN,ALEQUIS RIVAS,ELIZABETH NAVARRO,LAURA CORSINI,GINO
author_facet	PARADA,JOSÉ ATRIA,ANA MARÍA BAGGIO,RICARDO WIESE,GUILLERMO LAGOS,SEBASTIÁN PAVÓN,ALEQUIS RIVAS,ELIZABETH NAVARRO,LAURA CORSINI,GINO
author_sort	PARADA,JOSÉ
title	ANTIBACTERIAL ACTIVITY AND HUMAN CELL CYTOTOXIC OF COBALT (III) COMPLEXES WITH 1,10-PHENANTHROLINE AND CARBOHYDRATE LIGANDS
title_short	ANTIBACTERIAL ACTIVITY AND HUMAN CELL CYTOTOXIC OF COBALT (III) COMPLEXES WITH 1,10-PHENANTHROLINE AND CARBOHYDRATE LIGANDS
title_full	ANTIBACTERIAL ACTIVITY AND HUMAN CELL CYTOTOXIC OF COBALT (III) COMPLEXES WITH 1,10-PHENANTHROLINE AND CARBOHYDRATE LIGANDS
title_fullStr	ANTIBACTERIAL ACTIVITY AND HUMAN CELL CYTOTOXIC OF COBALT (III) COMPLEXES WITH 1,10-PHENANTHROLINE AND CARBOHYDRATE LIGANDS
title_full_unstemmed	ANTIBACTERIAL ACTIVITY AND HUMAN CELL CYTOTOXIC OF COBALT (III) COMPLEXES WITH 1,10-PHENANTHROLINE AND CARBOHYDRATE LIGANDS
title_sort	antibacterial activity and human cell cytotoxic of cobalt (iii) complexes with 1,10-phenanthroline and carbohydrate ligands
publisher	Sociedad Chilena de Química
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072017000403746
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ANTIBACTERIAL ACTIVITY AND HUMAN CELL CYTOTOXIC OF COBALT (III) COMPLEXES WITH 1,10-PHENANTHROLINE AND CARBOHYDRATE LIGANDS

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