STUDY OF DNA-DIETHYLSTILBOESTROL INTERACTION USING GRAPHENE-BASED THIN-FILM SENSOR AND UV-VIS SPECTROSCOPY

ABSTRACT The graphene-modified glassy carbon electrode (Gr/GCE) was fabricated by casting graphene (Gr). Some electrochemical parameters for diethylstilbestrol (DES) detection, such as pH, scan rate, and accumulation time were discussed. The interaction of DES with DNA at a Gr/GCE was studied by ele...

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Autores principales:	Chen,Meifeng, Ma,Xinying, Li,Xia, Yin,Mingjing, Li,Yanyun, Sun,Zhanghua
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2018
Materias:	diethylstilboestrol DNA interaction electrochemical method UV-Vis spectroscopy
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072018000103795
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id	oai:scielo:S0717-97072018000103795
record_format	dspace
spelling	oai:scielo:S0717-970720180001037952018-05-11STUDY OF DNA-DIETHYLSTILBOESTROL INTERACTION USING GRAPHENE-BASED THIN-FILM SENSOR AND UV-VIS SPECTROSCOPYChen,MeifengMa,XinyingLi,XiaYin,MingjingLi,YanyunSun,Zhanghua diethylstilboestrol DNA interaction electrochemical method UV-Vis spectroscopy ABSTRACT The graphene-modified glassy carbon electrode (Gr/GCE) was fabricated by casting graphene (Gr). Some electrochemical parameters for diethylstilbestrol (DES) detection, such as pH, scan rate, and accumulation time were discussed. The interaction of DES with DNA at a Gr/GCE was studied by electrochemical method and factors influencing the interaction were optimized. Under the optimized conditions, the results revealed that the peak current decreased and the peak potential shifted to the positive side after adding DNA into the solution containing DES. We deduced that the interaction of DES with DNA mainly is intercalation. The interaction of DES with DNA was studied by ultraviolet and visible (UV-Vis) absorption spectroscopy. Also, with the addition of DNA, hyperchromic effect and small red shifts were observed in its UV-VIS spectra upon addition of DNA, results indicated that the DES molecule intercalated into the DNA, and the complex of DES-DNA was formed.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.63 n.1 20182018-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072018000103795en10.4067/s0717-97072018000103795
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	diethylstilboestrol DNA interaction electrochemical method UV-Vis spectroscopy
spellingShingle	diethylstilboestrol DNA interaction electrochemical method UV-Vis spectroscopy Chen,Meifeng Ma,Xinying Li,Xia Yin,Mingjing Li,Yanyun Sun,Zhanghua STUDY OF DNA-DIETHYLSTILBOESTROL INTERACTION USING GRAPHENE-BASED THIN-FILM SENSOR AND UV-VIS SPECTROSCOPY
description	ABSTRACT The graphene-modified glassy carbon electrode (Gr/GCE) was fabricated by casting graphene (Gr). Some electrochemical parameters for diethylstilbestrol (DES) detection, such as pH, scan rate, and accumulation time were discussed. The interaction of DES with DNA at a Gr/GCE was studied by electrochemical method and factors influencing the interaction were optimized. Under the optimized conditions, the results revealed that the peak current decreased and the peak potential shifted to the positive side after adding DNA into the solution containing DES. We deduced that the interaction of DES with DNA mainly is intercalation. The interaction of DES with DNA was studied by ultraviolet and visible (UV-Vis) absorption spectroscopy. Also, with the addition of DNA, hyperchromic effect and small red shifts were observed in its UV-VIS spectra upon addition of DNA, results indicated that the DES molecule intercalated into the DNA, and the complex of DES-DNA was formed.
author	Chen,Meifeng Ma,Xinying Li,Xia Yin,Mingjing Li,Yanyun Sun,Zhanghua
author_facet	Chen,Meifeng Ma,Xinying Li,Xia Yin,Mingjing Li,Yanyun Sun,Zhanghua
author_sort	Chen,Meifeng
title	STUDY OF DNA-DIETHYLSTILBOESTROL INTERACTION USING GRAPHENE-BASED THIN-FILM SENSOR AND UV-VIS SPECTROSCOPY
title_short	STUDY OF DNA-DIETHYLSTILBOESTROL INTERACTION USING GRAPHENE-BASED THIN-FILM SENSOR AND UV-VIS SPECTROSCOPY
title_full	STUDY OF DNA-DIETHYLSTILBOESTROL INTERACTION USING GRAPHENE-BASED THIN-FILM SENSOR AND UV-VIS SPECTROSCOPY
title_fullStr	STUDY OF DNA-DIETHYLSTILBOESTROL INTERACTION USING GRAPHENE-BASED THIN-FILM SENSOR AND UV-VIS SPECTROSCOPY
title_full_unstemmed	STUDY OF DNA-DIETHYLSTILBOESTROL INTERACTION USING GRAPHENE-BASED THIN-FILM SENSOR AND UV-VIS SPECTROSCOPY
title_sort	study of dna-diethylstilboestrol interaction using graphene-based thin-film sensor and uv-vis spectroscopy
publisher	Sociedad Chilena de Química
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072018000103795
work_keys_str_mv	AT chenmeifeng studyofdnadiethylstilboestrolinteractionusinggraphenebasedthinfilmsensoranduvvisspectroscopy AT maxinying studyofdnadiethylstilboestrolinteractionusinggraphenebasedthinfilmsensoranduvvisspectroscopy AT lixia studyofdnadiethylstilboestrolinteractionusinggraphenebasedthinfilmsensoranduvvisspectroscopy AT yinmingjing studyofdnadiethylstilboestrolinteractionusinggraphenebasedthinfilmsensoranduvvisspectroscopy AT liyanyun studyofdnadiethylstilboestrolinteractionusinggraphenebasedthinfilmsensoranduvvisspectroscopy AT sunzhanghua studyofdnadiethylstilboestrolinteractionusinggraphenebasedthinfilmsensoranduvvisspectroscopy
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STUDY OF DNA-DIETHYLSTILBOESTROL INTERACTION USING GRAPHENE-BASED THIN-FILM SENSOR AND UV-VIS SPECTROSCOPY

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