HIGH THROUGHPUT ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY - MASS SPECTROMETRIC METHOD FOR DETERMINATION OF ADRENALINE AND CHARACTERIZATION OF ITS STRESS DEGRADATION PRODUCTS: A MECHANISTIC APPROACH

ABSTRACT UPLC-MS analytical procedure has been established for the determination of adrenaline in marketed formulation and in the pure form. The detection of the adrenaline was executed considering positive electrospray ionization (ESI) and selected ion reaction (SIR) modes. Chromatographic separati...

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Autores principales:	Siddiqui,Masoom R., Wabaidur,Saikh M., Alothman,Zeid A., Rafiquee,M.Z.A., Khan,Moonis Ali, Sumbul,Sadia
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2018
Materias:	Adrenaline UPLC-MS method pharmaceutical analysis forced degradation study
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072018000103803
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spelling	oai:scielo:S0717-970720180001038032018-05-11HIGH THROUGHPUT ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY - MASS SPECTROMETRIC METHOD FOR DETERMINATION OF ADRENALINE AND CHARACTERIZATION OF ITS STRESS DEGRADATION PRODUCTS: A MECHANISTIC APPROACHSiddiqui,Masoom R.Wabaidur,Saikh M.Alothman,Zeid A.Rafiquee,M.Z.A.Khan,Moonis AliSumbul,Sadia Adrenaline UPLC-MS method pharmaceutical analysis forced degradation study ABSTRACT UPLC-MS analytical procedure has been established for the determination of adrenaline in marketed formulation and in the pure form. The detection of the adrenaline was executed considering positive electrospray ionization (ESI) and selected ion reaction (SIR) modes. Chromatographic separation was achieved using ACQUITY UPLC BEH C column (100 mm × 2.1 mm, 1.7 μm particles size) using a binary mobile phase mixture of methanol and water (50:50). The mobile phase was set to flow at the rate of 0.3 ml min−1. The run time time for adrenaline was as short as 1.5 minutes. Linearity was found to be over the concentration range of 1μg ml−1 – 6μg ml−1. An excellent recovery of 100.18 % – 100.68 % was achieved using this procedure. The standard deviation ranged from 0.014 – 0.118 in intra-day studies and 0.016 – 0.096 in case of inter-day studies. The procedure's quantitation limit was 0.76μg ml−1 and detection limit was 0.25 μg ml−1. The method was successfully applied to determine adrenaline in drug formulations. In order to get the information about the stability of adrenaline in various environments forced degradation studies were conducted and the characterization of the stressed samples were performed using mass spectrometry.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.63 n.1 20182018-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072018000103803en10.4067/s0717-97072018000103803
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Adrenaline UPLC-MS method pharmaceutical analysis forced degradation study
spellingShingle	Adrenaline UPLC-MS method pharmaceutical analysis forced degradation study Siddiqui,Masoom R. Wabaidur,Saikh M. Alothman,Zeid A. Rafiquee,M.Z.A. Khan,Moonis Ali Sumbul,Sadia HIGH THROUGHPUT ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY - MASS SPECTROMETRIC METHOD FOR DETERMINATION OF ADRENALINE AND CHARACTERIZATION OF ITS STRESS DEGRADATION PRODUCTS: A MECHANISTIC APPROACH
description	ABSTRACT UPLC-MS analytical procedure has been established for the determination of adrenaline in marketed formulation and in the pure form. The detection of the adrenaline was executed considering positive electrospray ionization (ESI) and selected ion reaction (SIR) modes. Chromatographic separation was achieved using ACQUITY UPLC BEH C column (100 mm × 2.1 mm, 1.7 μm particles size) using a binary mobile phase mixture of methanol and water (50:50). The mobile phase was set to flow at the rate of 0.3 ml min−1. The run time time for adrenaline was as short as 1.5 minutes. Linearity was found to be over the concentration range of 1μg ml−1 – 6μg ml−1. An excellent recovery of 100.18 % – 100.68 % was achieved using this procedure. The standard deviation ranged from 0.014 – 0.118 in intra-day studies and 0.016 – 0.096 in case of inter-day studies. The procedure's quantitation limit was 0.76μg ml−1 and detection limit was 0.25 μg ml−1. The method was successfully applied to determine adrenaline in drug formulations. In order to get the information about the stability of adrenaline in various environments forced degradation studies were conducted and the characterization of the stressed samples were performed using mass spectrometry.
author	Siddiqui,Masoom R. Wabaidur,Saikh M. Alothman,Zeid A. Rafiquee,M.Z.A. Khan,Moonis Ali Sumbul,Sadia
author_facet	Siddiqui,Masoom R. Wabaidur,Saikh M. Alothman,Zeid A. Rafiquee,M.Z.A. Khan,Moonis Ali Sumbul,Sadia
author_sort	Siddiqui,Masoom R.
title	HIGH THROUGHPUT ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY - MASS SPECTROMETRIC METHOD FOR DETERMINATION OF ADRENALINE AND CHARACTERIZATION OF ITS STRESS DEGRADATION PRODUCTS: A MECHANISTIC APPROACH
title_short	HIGH THROUGHPUT ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY - MASS SPECTROMETRIC METHOD FOR DETERMINATION OF ADRENALINE AND CHARACTERIZATION OF ITS STRESS DEGRADATION PRODUCTS: A MECHANISTIC APPROACH
title_full	HIGH THROUGHPUT ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY - MASS SPECTROMETRIC METHOD FOR DETERMINATION OF ADRENALINE AND CHARACTERIZATION OF ITS STRESS DEGRADATION PRODUCTS: A MECHANISTIC APPROACH
title_fullStr	HIGH THROUGHPUT ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY - MASS SPECTROMETRIC METHOD FOR DETERMINATION OF ADRENALINE AND CHARACTERIZATION OF ITS STRESS DEGRADATION PRODUCTS: A MECHANISTIC APPROACH
title_full_unstemmed	HIGH THROUGHPUT ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY - MASS SPECTROMETRIC METHOD FOR DETERMINATION OF ADRENALINE AND CHARACTERIZATION OF ITS STRESS DEGRADATION PRODUCTS: A MECHANISTIC APPROACH
title_sort	high throughput ultra performance liquid chromatography - mass spectrometric method for determination of adrenaline and characterization of its stress degradation products: a mechanistic approach
publisher	Sociedad Chilena de Química
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072018000103803
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HIGH THROUGHPUT ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY - MASS SPECTROMETRIC METHOD FOR DETERMINATION OF ADRENALINE AND CHARACTERIZATION OF ITS STRESS DEGRADATION PRODUCTS: A MECHANISTIC APPROACH

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