DISPERSIVE LIQUID-LIQUID MICROEXTRACTION COUPLED WITH MICRO-VOLUME SPECTROPHOTOMETRY FOR DETERMINATION OF TOTAL IODINE IN URINE SAMPLES

ABSTRACT This work describes a simple photometric determination of iodine concentrations in urine using a dispersive liquid-liquid microextraction method. After digestion of the urine samples with ammonium persulfate, iodide was oxidized with nitrous acid to ICl2– anion at concentrated hyd...

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Autores principales:	Kaykhaei,Mahmood Ali, Kaykhaii,Massoud, Hashemi,Mohammad, Sargazi,Mona
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2018
Materias:	Urinary iodine Methods comparison Dispersive liquid-liquid microextraction
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072018000203947
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spelling	oai:scielo:S0717-970720180002039472018-07-19DISPERSIVE LIQUID-LIQUID MICROEXTRACTION COUPLED WITH MICRO-VOLUME SPECTROPHOTOMETRY FOR DETERMINATION OF TOTAL IODINE IN URINE SAMPLESKaykhaei,Mahmood AliKaykhaii,MassoudHashemi,MohammadSargazi,Mona Urinary iodine Methods comparison Dispersive liquid-liquid microextraction ABSTRACT This work describes a simple photometric determination of iodine concentrations in urine using a dispersive liquid-liquid microextraction method. After digestion of the urine samples with ammonium persulfate, iodide was oxidized with nitrous acid to ICl2– anion at concentrated hydrochloric acid, then Rhodamine B is added and the ion-pair formed was extracted into a mixture of toluene (extracting phase) and ethanol (dispersive solvent) and measured spectrophotometrically. By means of this sensitive method, iodine concentrations can be determined in very small amount of urine specimens (250 μL). The calibration curve showed good linearity within the range of 100-900 μg.L–1 (R2 = 0.985), and the limit of detection for urine was 33.3 μg.L–1. The mean recovery of urine were 95.9-101.2 % with RSD of 2.6-6.6 %. The accuracy and precision of the method was evaluated by comparing to the standard Kolthoff-Sandell method. This suggested method affords a number of advantages, such as being simple, rapid, and inexpensive, with low organic solvent consumption, and is remarkably free from interference effects, rendering it an efficient method for the determination of iodine in urine samples in any routine clinical laboratory.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.63 n.2 20182018-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072018000203947en10.4067/s0717-97072018000203947
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Urinary iodine Methods comparison Dispersive liquid-liquid microextraction
spellingShingle	Urinary iodine Methods comparison Dispersive liquid-liquid microextraction Kaykhaei,Mahmood Ali Kaykhaii,Massoud Hashemi,Mohammad Sargazi,Mona DISPERSIVE LIQUID-LIQUID MICROEXTRACTION COUPLED WITH MICRO-VOLUME SPECTROPHOTOMETRY FOR DETERMINATION OF TOTAL IODINE IN URINE SAMPLES
description	ABSTRACT This work describes a simple photometric determination of iodine concentrations in urine using a dispersive liquid-liquid microextraction method. After digestion of the urine samples with ammonium persulfate, iodide was oxidized with nitrous acid to ICl2– anion at concentrated hydrochloric acid, then Rhodamine B is added and the ion-pair formed was extracted into a mixture of toluene (extracting phase) and ethanol (dispersive solvent) and measured spectrophotometrically. By means of this sensitive method, iodine concentrations can be determined in very small amount of urine specimens (250 μL). The calibration curve showed good linearity within the range of 100-900 μg.L–1 (R2 = 0.985), and the limit of detection for urine was 33.3 μg.L–1. The mean recovery of urine were 95.9-101.2 % with RSD of 2.6-6.6 %. The accuracy and precision of the method was evaluated by comparing to the standard Kolthoff-Sandell method. This suggested method affords a number of advantages, such as being simple, rapid, and inexpensive, with low organic solvent consumption, and is remarkably free from interference effects, rendering it an efficient method for the determination of iodine in urine samples in any routine clinical laboratory.
author	Kaykhaei,Mahmood Ali Kaykhaii,Massoud Hashemi,Mohammad Sargazi,Mona
author_facet	Kaykhaei,Mahmood Ali Kaykhaii,Massoud Hashemi,Mohammad Sargazi,Mona
author_sort	Kaykhaei,Mahmood Ali
title	DISPERSIVE LIQUID-LIQUID MICROEXTRACTION COUPLED WITH MICRO-VOLUME SPECTROPHOTOMETRY FOR DETERMINATION OF TOTAL IODINE IN URINE SAMPLES
title_short	DISPERSIVE LIQUID-LIQUID MICROEXTRACTION COUPLED WITH MICRO-VOLUME SPECTROPHOTOMETRY FOR DETERMINATION OF TOTAL IODINE IN URINE SAMPLES
title_full	DISPERSIVE LIQUID-LIQUID MICROEXTRACTION COUPLED WITH MICRO-VOLUME SPECTROPHOTOMETRY FOR DETERMINATION OF TOTAL IODINE IN URINE SAMPLES
title_fullStr	DISPERSIVE LIQUID-LIQUID MICROEXTRACTION COUPLED WITH MICRO-VOLUME SPECTROPHOTOMETRY FOR DETERMINATION OF TOTAL IODINE IN URINE SAMPLES
title_full_unstemmed	DISPERSIVE LIQUID-LIQUID MICROEXTRACTION COUPLED WITH MICRO-VOLUME SPECTROPHOTOMETRY FOR DETERMINATION OF TOTAL IODINE IN URINE SAMPLES
title_sort	dispersive liquid-liquid microextraction coupled with micro-volume spectrophotometry for determination of total iodine in urine samples
publisher	Sociedad Chilena de Química
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072018000203947
work_keys_str_mv	AT kaykhaeimahmoodali dispersiveliquidliquidmicroextractioncoupledwithmicrovolumespectrophotometryfordeterminationoftotaliodineinurinesamples AT kaykhaiimassoud dispersiveliquidliquidmicroextractioncoupledwithmicrovolumespectrophotometryfordeterminationoftotaliodineinurinesamples AT hashemimohammad dispersiveliquidliquidmicroextractioncoupledwithmicrovolumespectrophotometryfordeterminationoftotaliodineinurinesamples AT sargazimona dispersiveliquidliquidmicroextractioncoupledwithmicrovolumespectrophotometryfordeterminationoftotaliodineinurinesamples
_version_	1714200924745891840

DISPERSIVE LIQUID-LIQUID MICROEXTRACTION COUPLED WITH MICRO-VOLUME SPECTROPHOTOMETRY FOR DETERMINATION OF TOTAL IODINE IN URINE SAMPLES

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