SPECTROPHOTOMETRIC DETERMINATION OF REDUCING SUGAR IN WINES EMPLOYING IN-LINE DIALYSIS AND A MULTICOMMUTED FLOW ANALYSIS APPROACH

ABSTRACT A multicommuted flow procedure for the spectrophotometric determination of reducing sugars in wine has been developed. A dialysis unit was incorporated in the system to minimize matrix interferences and eliminate the colored compounds of the wine. The oxidation-reduction reaction in an alka...

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Autores principales:	SILVA,PAULO A. B. DA, SOUZA,GUSTAVO C. S. DE, PAIM,ANA PAULA S., LAVORANTE,ANDRÉ F.
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2018
Materias:	Dialysis Flow injection analysis Multicommutation Reducing sugar Wine
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072018000203994
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spelling	oai:scielo:S0717-970720180002039942018-07-19SPECTROPHOTOMETRIC DETERMINATION OF REDUCING SUGAR IN WINES EMPLOYING IN-LINE DIALYSIS AND A MULTICOMMUTED FLOW ANALYSIS APPROACHSILVA,PAULO A. B. DASOUZA,GUSTAVO C. S. DEPAIM,ANA PAULA S.LAVORANTE,ANDRÉ F. Dialysis Flow injection analysis Multicommutation Reducing sugar Wine ABSTRACT A multicommuted flow procedure for the spectrophotometric determination of reducing sugars in wine has been developed. A dialysis unit was incorporated in the system to minimize matrix interferences and eliminate the colored compounds of the wine. The oxidation-reduction reaction in an alkaline medium between reducing sugars and the Cu2+-Neocuproine complex was employed. The compound formed was monitored at 460 nm. In the proposed procedure, there was a linear response between 0.25 and 4.0 g L-1 reducing sugars (y = (0.36 ± 0.02) + (0.31 ± 0.01) * x, R = 0.9975, n = 6), a coefficient of variance of 2.6% for a reducing sugar solution of 2.0 g L-1 (n = 7), a limit of detection estimated at 0.03 g L-1, reagent consumption per determination of 0.330, 0.128 and 0.160 mg for neocuproine, CuSO4 and NaOH, respectively. A sampling throughput of 45 determinations per hour was achieved. The proposed system was applied to wine samples and comparing the results with an AOAC official method no significant difference at a 95% confidence level.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.63 n.2 20182018-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072018000203994en10.4067/s0717-97072018000203994
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Dialysis Flow injection analysis Multicommutation Reducing sugar Wine
spellingShingle	Dialysis Flow injection analysis Multicommutation Reducing sugar Wine SILVA,PAULO A. B. DA SOUZA,GUSTAVO C. S. DE PAIM,ANA PAULA S. LAVORANTE,ANDRÉ F. SPECTROPHOTOMETRIC DETERMINATION OF REDUCING SUGAR IN WINES EMPLOYING IN-LINE DIALYSIS AND A MULTICOMMUTED FLOW ANALYSIS APPROACH
description	ABSTRACT A multicommuted flow procedure for the spectrophotometric determination of reducing sugars in wine has been developed. A dialysis unit was incorporated in the system to minimize matrix interferences and eliminate the colored compounds of the wine. The oxidation-reduction reaction in an alkaline medium between reducing sugars and the Cu2+-Neocuproine complex was employed. The compound formed was monitored at 460 nm. In the proposed procedure, there was a linear response between 0.25 and 4.0 g L-1 reducing sugars (y = (0.36 ± 0.02) + (0.31 ± 0.01) * x, R = 0.9975, n = 6), a coefficient of variance of 2.6% for a reducing sugar solution of 2.0 g L-1 (n = 7), a limit of detection estimated at 0.03 g L-1, reagent consumption per determination of 0.330, 0.128 and 0.160 mg for neocuproine, CuSO4 and NaOH, respectively. A sampling throughput of 45 determinations per hour was achieved. The proposed system was applied to wine samples and comparing the results with an AOAC official method no significant difference at a 95% confidence level.
author	SILVA,PAULO A. B. DA SOUZA,GUSTAVO C. S. DE PAIM,ANA PAULA S. LAVORANTE,ANDRÉ F.
author_facet	SILVA,PAULO A. B. DA SOUZA,GUSTAVO C. S. DE PAIM,ANA PAULA S. LAVORANTE,ANDRÉ F.
author_sort	SILVA,PAULO A. B. DA
title	SPECTROPHOTOMETRIC DETERMINATION OF REDUCING SUGAR IN WINES EMPLOYING IN-LINE DIALYSIS AND A MULTICOMMUTED FLOW ANALYSIS APPROACH
title_short	SPECTROPHOTOMETRIC DETERMINATION OF REDUCING SUGAR IN WINES EMPLOYING IN-LINE DIALYSIS AND A MULTICOMMUTED FLOW ANALYSIS APPROACH
title_full	SPECTROPHOTOMETRIC DETERMINATION OF REDUCING SUGAR IN WINES EMPLOYING IN-LINE DIALYSIS AND A MULTICOMMUTED FLOW ANALYSIS APPROACH
title_fullStr	SPECTROPHOTOMETRIC DETERMINATION OF REDUCING SUGAR IN WINES EMPLOYING IN-LINE DIALYSIS AND A MULTICOMMUTED FLOW ANALYSIS APPROACH
title_full_unstemmed	SPECTROPHOTOMETRIC DETERMINATION OF REDUCING SUGAR IN WINES EMPLOYING IN-LINE DIALYSIS AND A MULTICOMMUTED FLOW ANALYSIS APPROACH
title_sort	spectrophotometric determination of reducing sugar in wines employing in-line dialysis and a multicommuted flow analysis approach
publisher	Sociedad Chilena de Química
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072018000203994
work_keys_str_mv	AT silvapauloabda spectrophotometricdeterminationofreducingsugarinwinesemployinginlinedialysisandamulticommutedflowanalysisapproach AT souzagustavocsde spectrophotometricdeterminationofreducingsugarinwinesemployinginlinedialysisandamulticommutedflowanalysisapproach AT paimanapaulas spectrophotometricdeterminationofreducingsugarinwinesemployinginlinedialysisandamulticommutedflowanalysisapproach AT lavoranteandref spectrophotometricdeterminationofreducingsugarinwinesemployinginlinedialysisandamulticommutedflowanalysisapproach
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SPECTROPHOTOMETRIC DETERMINATION OF REDUCING SUGAR IN WINES EMPLOYING IN-LINE DIALYSIS AND A MULTICOMMUTED FLOW ANALYSIS APPROACH

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