EVALUATION OF MONOLITHIC COLUMN FOR INORGANIC MERCURY AND METHYLMERCURY DETERMINATION IN FISH SAMPLE ANALYSIS

ABSTRACT In the present work, the applicability of a monolithic column was evaluated for mercury speciation analysis in certified and real fish samples. The influence of the flow and composition of the mobile phase (2-mercaptoethanol concentration and methanol content) on the retention times of iHg...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Bravo,Manuel A., Riquelme,Ornella, Parra,Sonnia, Bergmann,Jan, Quiroz,Waldo
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2018
Materias:	Mercury speciation Monolithic column Fish samples
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072018000404257
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-97072018000404257
record_format	dspace
spelling	oai:scielo:S0717-970720180004042572019-02-07EVALUATION OF MONOLITHIC COLUMN FOR INORGANIC MERCURY AND METHYLMERCURY DETERMINATION IN FISH SAMPLE ANALYSISBravo,Manuel A.Riquelme,OrnellaParra,SonniaBergmann,JanQuiroz,Waldo Mercury speciation Monolithic column Fish samples ABSTRACT In the present work, the applicability of a monolithic column was evaluated for mercury speciation analysis in certified and real fish samples. The influence of the flow and composition of the mobile phase (2-mercaptoethanol concentration and methanol content) on the retention times of iHg and MeHg was evaluated. The optimal separation conditions were achieved for a mobile phase containing 5 % methanol and 0.02 % mercaptoethanol in acetate buffer (pH 5.0) at a flow rate of 1.6 ml min−1. Different extraction conditions were studied to analyze a certified reference material with the optimized methodology and the best results (93 % recovery) were obtained for microwave-assisted extraction with a mixture of HCl 6 M + NaCl 0.5 M. Finally, the proposed method was satisfactorily applied to real fish samples collected from different markets in the central region of Chile, presenting levels ranging from 0.73 – 3.4 mg kg−1.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.63 n.4 20182018-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072018000404257en10.4067/S0717-97072018000404257
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Mercury speciation Monolithic column Fish samples
spellingShingle	Mercury speciation Monolithic column Fish samples Bravo,Manuel A. Riquelme,Ornella Parra,Sonnia Bergmann,Jan Quiroz,Waldo EVALUATION OF MONOLITHIC COLUMN FOR INORGANIC MERCURY AND METHYLMERCURY DETERMINATION IN FISH SAMPLE ANALYSIS
description	ABSTRACT In the present work, the applicability of a monolithic column was evaluated for mercury speciation analysis in certified and real fish samples. The influence of the flow and composition of the mobile phase (2-mercaptoethanol concentration and methanol content) on the retention times of iHg and MeHg was evaluated. The optimal separation conditions were achieved for a mobile phase containing 5 % methanol and 0.02 % mercaptoethanol in acetate buffer (pH 5.0) at a flow rate of 1.6 ml min−1. Different extraction conditions were studied to analyze a certified reference material with the optimized methodology and the best results (93 % recovery) were obtained for microwave-assisted extraction with a mixture of HCl 6 M + NaCl 0.5 M. Finally, the proposed method was satisfactorily applied to real fish samples collected from different markets in the central region of Chile, presenting levels ranging from 0.73 – 3.4 mg kg−1.
author	Bravo,Manuel A. Riquelme,Ornella Parra,Sonnia Bergmann,Jan Quiroz,Waldo
author_facet	Bravo,Manuel A. Riquelme,Ornella Parra,Sonnia Bergmann,Jan Quiroz,Waldo
author_sort	Bravo,Manuel A.
title	EVALUATION OF MONOLITHIC COLUMN FOR INORGANIC MERCURY AND METHYLMERCURY DETERMINATION IN FISH SAMPLE ANALYSIS
title_short	EVALUATION OF MONOLITHIC COLUMN FOR INORGANIC MERCURY AND METHYLMERCURY DETERMINATION IN FISH SAMPLE ANALYSIS
title_full	EVALUATION OF MONOLITHIC COLUMN FOR INORGANIC MERCURY AND METHYLMERCURY DETERMINATION IN FISH SAMPLE ANALYSIS
title_fullStr	EVALUATION OF MONOLITHIC COLUMN FOR INORGANIC MERCURY AND METHYLMERCURY DETERMINATION IN FISH SAMPLE ANALYSIS
title_full_unstemmed	EVALUATION OF MONOLITHIC COLUMN FOR INORGANIC MERCURY AND METHYLMERCURY DETERMINATION IN FISH SAMPLE ANALYSIS
title_sort	evaluation of monolithic column for inorganic mercury and methylmercury determination in fish sample analysis
publisher	Sociedad Chilena de Química
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072018000404257
work_keys_str_mv	AT bravomanuela evaluationofmonolithiccolumnforinorganicmercuryandmethylmercurydeterminationinfishsampleanalysis AT riquelmeornella evaluationofmonolithiccolumnforinorganicmercuryandmethylmercurydeterminationinfishsampleanalysis AT parrasonnia evaluationofmonolithiccolumnforinorganicmercuryandmethylmercurydeterminationinfishsampleanalysis AT bergmannjan evaluationofmonolithiccolumnforinorganicmercuryandmethylmercurydeterminationinfishsampleanalysis AT quirozwaldo evaluationofmonolithiccolumnforinorganicmercuryandmethylmercurydeterminationinfishsampleanalysis
_version_	1714200932941561856

EVALUATION OF MONOLITHIC COLUMN FOR INORGANIC MERCURY AND METHYLMERCURY DETERMINATION IN FISH SAMPLE ANALYSIS

Ejemplares similares